Labshake search
Citations for Qiagen :
2401 - 2450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... and genomic DNA was extracted using a QIAamp DNA Blood Mini Kit (QIAGEN, Germany; 51126). The exomes of the subjects were captured by Agilent SureSelect Human All Exon V6 Enrichment kits (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen). The FOXP3 TSDR regions were then amplified from the converted sample gDNAs by qPCR using specific primers (forward ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated using RNEasy Mini kit (Qiagen, catalog number 74104).
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted and purified using QIAzol (Qiagen) and ReliaPrep RNA Cell MiniPrep System (Promega) ...
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bioRxiv - Cell Biology 2024Quote: The samples were processed according to manufacturer protocols (Qproteome Mitochondria Isolation Kit, Qiagen). From the different steps were obtained three different fractions ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmid DNAs were extracted with QIAGEN Plasmid Plus MIDI kit (QIAGEN 12941) for downstream applications.
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from cell pellets by the QIAamp Blood Maxi Kit (Qiagen) and resuspended in Buffer EB (10 mM Tris–HCl [pH 7.5] ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X106 cells were mixed with 5 μl of 20 μM siRNAs (GeneSolution siRNA, Qiagen) and 100 μl of Ingenio electroporation solution (Mirus ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted from sEVs fractions using the Qiagen miRNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA extraction from the samples was conducted using the miRNeasy Kit (Qiagen), according to standard operating procedures provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected by double centrifugation and 6x His-tagged target protein in the supernatant was purified using the medium by Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated via QIAzol-chloroform extraction followed by column-based purification and On-Column DNase Digestion (Qiagen #79254). The aqueous phase was brought to a final concentration of 50% ethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA was purified using the RNeasy Lipid Tissue Mini Kit according to the manufacturer’s instructions (Qiagen #74804). Library preparation was performed using the Illumina TruSeq Stranded mRNA Library Prep with UDI (Illumina ...
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bioRxiv - Immunology 2024Quote: ... RNA was further isolated with a RNAeasy mini kit (Qiagen). The RNA (1μg ...
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bioRxiv - Immunology 2024Quote: ... 10,000 cells were lysed in 600uL RLT lysis buffer (Qiagen Cat#79216) + 1% BME ...
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bioRxiv - Immunology 2024Quote: ... Sorted cells (2-4x104 per sample) were resuspended in 350 µl RNeasy Lysis Buffer (QIAGEN), and RNA was extracted using the QIAGEN RNeasy Mini kit (QIAGEN) ...
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bioRxiv - Immunology 2024Quote: ... and RNA was extracted using the QIAGEN RNeasy Mini kit (QIAGEN). RNA was washed ...
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bioRxiv - Immunology 2024Quote: ... treated with DNase I (QIAGEN), and eluted per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 500 µL of each culture was immediately fixed in the RNAprotect Bacteria Reagent (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA was extracted using the RNeasy Mini Kit (Qiagen) with on-column DNAse treatment ...
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bioRxiv - Cell Biology 2024Quote: ... and RNeasy Micro RNA extraction kit (Qiagen). Reverse transcription was then carried out to obtain corresponding cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Pathology 2024Quote: Mouse kidney outer cortex tissues were dissected and homogenized in QIAzol (QIAGEN, Germantown, MD). Total RNA samples were extracted using RNeasy Plus Universal Kit (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA constructs were then verified by Sanger sequencing and amplified by maxiprep (Qiagen #12162). Lentiviruses were produced by co-transfection of 293T/17 cells with pVSV-G and pdVPR29.1 plasmids ...
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bioRxiv - Genomics 2024Quote: ... 100 mg of leaves were collected and DNA was extracted from either lyophilised or fresh leaves using the Qiagen 96 Plant Kit (Qiagen, Hilden, Germany). DNA quality and quantity were checked with the Nanodrop technologies and Qubit kit prior to sending samples to the different facilities.
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was mixed with 5 ml of Ni-NTA agarose (QIAGEN) pre-equilibrated with solubilization buffer for 60 min ...
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bioRxiv - Immunology 2024Quote: ... The remaining colonic tissue and mesenteric lymph nodes were stored in RNAprotect Tissue Reagent (Qiagen, Hilden ...
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bioRxiv - Microbiology 2024Quote: ... An 80 μL sample of the supernatant was transferred to a new tube and incubated with 5 μL of 5 mg/mL Proteinase K (QIAGEN) for 1 h at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... the soil and sediment samples were extracted using the RNeasy® PowerSoil® Total RNA Kit and RNeasy® PowerSoil® DNA Elution Kit (QIAGEN, Germany), while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... as per manufacturer’s instructions (Qiagen QIAamp DNA blood minikit). The DNA concentration of replicates was determined using the Qubit 2.0 fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN, Germany). The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was purified using QIAGEN PCR purification Kit (QIAGEN, 28106). Libraries were amplified for N-1 cycles (being N the optimum Cq determined by qPCR reaction ...
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bioRxiv - Cell Biology 2024Quote: ... followed by column-based purification (Qiagen RNeasy Kit). cDNA was generated from 250-1000 ng of total RNA using an iScript™ cDNA Synthesis Kit (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA synthesis was performed on the Bio-Rad T100 PCR Gradient Thermal Cycler using the QuantiTect® Reverse Transcription kit (Qiagen, Germantown, MD), following manufactureŕs instructions.
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bioRxiv - Plant Biology 2024Quote: ... The leaf discs were disrupted using a TissueLyser LT (QIAGEN) bead mill for 3 min at 50 Hz ...
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bioRxiv - Microbiology 2024Quote: ... the phage was treated with DNase I (Qiagen) and RNase A (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA from the isolates was extracted using the Gentra Puregene Yeast/Bact Kit (Qiagen) and used as template for PCRs ...
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bioRxiv - Evolutionary Biology 2024Quote: Adult insects stored at -80°C were crushed (between 80 mg to 4650 mg of insects depending on the species) with a “Tissue-Lyser” apparatus (Qiagen) in a buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The total RNA of single adult females was extracted using the RNeasy Mini Kit (QIAGEN) and DNAse treated following manufacturer’s instructions (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: IPA (Qiagen) analysis was performed to elucidate canonical pathways ...
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bioRxiv - Biochemistry 2024Quote: ... coli (QIAGEN) or phage T4 SSB (gp32 ...
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bioRxiv - Cell Biology 2024Quote: ... with 2U HotStar Taq polymerase (Qiagen), PCR Buffer 10x (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... using DNase treatment (QIAGEN). RNA was analyzed using a 2100 Bioanalyzer (Aglient Technologies) ...
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bioRxiv - Genomics 2024Quote: RNeasy Plus Mini Kit (Qiagen, Hilden, Germany; cat. no. 74134)
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bioRxiv - Genomics 2024Quote: ... and QIAshredder kits (Qiagen, 79656) for VP64 and KRAB gRNA validations in SNRPN-2A-GFP iPSCs ...
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bioRxiv - Microbiology 2024Quote: ... using the QIAcube (Qiagen) automated sample preparation system ...
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bioRxiv - Immunology 2024Quote: ... with on-column DNase I digestion (Qiagen, #79254). RNA was suspended in 10 µL of nuclease free water at 0.5-1 ng/µL and sequenced using SMARTSeq277 at the Broad Institute Technology Labs and the Broad Genomics Platform ...
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bioRxiv - Microbiology 2024Quote: ... Kit (Qiagen) using 5 mL culture of V_227 which was grown in MMT-YE medium (20°C ...
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bioRxiv - Microbiology 2024Quote: ... Transcribed RNA was purified using RNeasy Mini Kit (Qiagen) and then transfected in RD cells using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from tissue lysate by RNAEasy Mini column purification (Qiagen) following the manufacturer’s instructions ...