Labshake search
Citations for Qiagen :
1901 - 1924 of 1924 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA (gDNA) was prepared in a plasmid- and PCR product-free work area using a QIAamp DNA Blood Maxi Kit (Qiagen, Cat. #51192) according to manufacturer instructions and previously published protocols27 ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Bioengineering 2023Quote: ... which were cultivated on a rotary shaker at 37° for 6 h before plasmid isolation using a Midi Kit (Qiagen, Hilden, Germany). The obtained plasmid libraries were subsequently used for electroporation of C ...
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bioRxiv - Systems Biology 2023Quote: DNA from Input and Output samples was extracted by first spheroblasting the yeast cells followed by plasmid Mini- or Midiprep according to the culture size using Qiagen kits (QIAGEN, Hilden, Germany). To spheroblast the cells ...
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bioRxiv - Genetics 2023Quote: ... 1 mL glycerol stocks were made for each library and a 200 mL LB-AMP outgrowth was grown to a concentration of 3 OD600/mL followed by a Qiagen MAXI plasmid prep (Qiagen Cat#12963).
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit, Qiagen Germantown, MD, USA) and ran them in the tissue lyser at either ½ speed or max speed for 30 sec or 1 min ...
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bioRxiv - Cancer Biology 2023Quote: ... An equal volume of inoculum from each pool was then combined and used as starter culture for a single maxiprep plasmid isolation of the pooled HDR template library (Qiagen Maxiprep kit).
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bioRxiv - Biochemistry 2024Quote: Cultures before and after selection were spun down and we lysed the cells and performed a standard plasmid extraction protocol using QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany). Illumina amplicons were generated by PCR of the barcode region ...
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bioRxiv - Neuroscience 2024Quote: ... Pooled plasmids were transformed into Endura electrocompetent cells (LGC, Middlesex, UK) and plasmid isolation performed using Qiagen Giga Prep Endofree kits (QIAGEN, Germantown, MA). For the second round library generation ...
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bioRxiv - Cell Biology 2021Quote: ... the ViiA 7™ Real-Time PCR system was used to run the Human Stem Cell RT² Profiler™ PCR Array (Qiagen, Hilden, Germany). For the analysis of cardiac differentiation-associated genes ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were cleaned up to remove unamplified circular plasmid template by gel extraction followed by digestion with DpnI and a PCR cleanup reaction (Qiagen PCR Purification kit). Mat a yeast were then transformed with the cloNAT resistance cassette ...
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bioRxiv - Genetics 2021Quote: ... Isolation of the plasmid DNA from bacterial clones was conducted using Zymo or Qiagen mini prep and maxi prep kits (Qiagen #27104, Zymo #D4208T)
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bioRxiv - Genetics 2020Quote: ... we grew one aliquot overnight in 400 mL LB + carbenicillin medium at 30°C and performed three maxi preps and two mega preps (200 mL each) using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965, #12981). For the Upstream library ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were plated at a density of ∼100,000 cells/well in a 24 well plate and transfected the following day with 500 ng of midi-prepped endotoxin-free plasmid DNA (Qiagen, Germantown, MD, USA) using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with 500 ng of complementation plasmid (described before) + 25 ng of pCoBlast (Thermo Fischer, Cat. No K5150-01) using Effectene Transfection Reagent (QIAgen, Cat. No 301425). 48 h after transfection ...
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bioRxiv - Genetics 2022Quote: ... the PCR fragment and the plasmid fragment without PDS gene were isolated from agarose gel using QIAquick Gel Extraction Kit (Qiagen, catalog number 28706). The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... 5µg of plasmid pLentiGuide-Puro was digested with BmsBI (Fermentas, Cat No. ER0451) and purified using the QIAquick Gel Extraction Kit (Qiagen, Cat No. 28704). Each pair of oligos was annealed and phosphorylated with T4 PNK (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... The plasmid library pool was isolated from overnight bacteria culture using QIAGEN HiSpeed Plasmid Midi (small pooled library) of maxi (TSS tiling library) kits (QIAGEN, 12643 and 12662). Lentivirus was produced from cloned plasmid pools in 293FT cells as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was eluted with 66 µL of 2 mM Tris-HCl pH=7.0 to carry out Solution A purification or eluted with 66 µL of 0.1 X EB buffer (From QIAGEN Plasmid Plus Midi Kit) without further Solution A purification.
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Molecular Biology 2024Quote: ... Liquid cultures were grown at 37°C overnight to saturation and DNA was extracted using QIAGEN® Plasmid Plus Midi Kit (Qiagen cat#12945)
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163; Qiagen, Redwood City, CA, USA). Lentivirus particles were produced in HEK293T cells after co-transfection of lentivirus plasmid vectors with packaging plasmids (VSVG and lenti gag/pol ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA of cells co-transfected with a plasmid expressing sgRNA and another expressing SpCas9 was isolated and purified through a commercially available kit (Qiagen, QIAamp DNA Mini Kit Cat# 51304) as per the manufacturer’s protocol ...