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Citations for Qiagen :
1851 - 1900 of 1924 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Colonies growing on the selective medium containing the antibiotic gentamycin (20 µg/ml) were checked by colony PCR and the plasmid DNA extracted using the QIAprep® Spin Miniprep kit (Qiagen). A restriction reaction was performed on the extracted plasmid DNA and the positive samples were sequenced ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Cell Biology 2022Quote: ... HT-1080 cells and the g2-41 Gp78 CRISPR/Cas9 knockout clones were stably transfected with mRFP-GFP tandem fluorescent-tagged LC3 (tfLC3) plasmid using Effectene (Cat. #301425, Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Molecular Biology 2021Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Molecular Biology 2021Quote: ... 2000 ng of plasmid DNA was transfected into each well using Effectene (16 μL of enhancer and 30 μL of Effectene reagent; Qiagen 301427). As above ...
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bioRxiv - Plant Biology 2019Quote: ... Bacteria were grown overnight at 37°C on LB plates with 50 μg/mL kanamycin and plasmids from randomly selected positive clones were purified using QIAprep Spin Miniprep kit (QIAGEN, Germany) and Sanger sequenced (Genoscreen ...
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bioRxiv - Genetics 2020Quote: ... and bands at 700 bp (insert) or 6 kb (plasmid library) cut and purified using QiaQuick Gel Extraction Kit (Qiagen #28706) with elution into 30 µL EB.
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... pCDNA-SARC-CoV-2 Wuhan-Hu-1 P681H S plasmid was then extracted using the QIAprep Spin Miniprep Kit (Qiagen N.V.) and Sanger sequencing was used to confirm incorporation of the mutation.
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bioRxiv - Cancer Biology 2021Quote: ... or CRTC3 or control sgRNA (Supplementary Table 2) were transfected into 293FT cells together with packaging plasmids pMD2.G and pSPAX2 using Effectene transfection reagent (Qiagen #301425). The viral supernatants were collected at 48 ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid sgRNA CRISPR porthos was co-transfected (1 µg of total DNA per well) with Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with the plasmid containing the Cdt1 gRNA/Cas9 and the Cdt1 repair template at equimolar ratio using Effectene (Qiagen, #301425) transfection agent ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro mRNA transcription was performed from linearized plasmids using the mMessage mMachine SP6 Transcription Kit (Fisher, #AM1340) and purified using the RNeasy Mini Kit (Qiagen, #74104). RNA transcript sizes were confirmed using 1% agarose gels containing 0.05% bleach and Millennium RNA markers (Fisher ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... Clones were verified by sequencing (ACGT; Wheeling, IL) and grown for midi-scale production and purification using a Qiagen Plasmid Plus Midiprep kit (Qiagen 12943).
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bioRxiv - Cancer Biology 2022Quote: ... These specific colonies were amplified by culturing in liquid LB-medium with ampicillin (1:1000) followed by plasmid isolation using QIAprep Spin Miniprep Kit (Qiagen #27104), according to the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... while all primers used for the construction of plasmids were designed using j5 software.43–45 Plasmids were assembled via Gibson Assembly using standard protocols,46 or Golden Gate Assembly using standard protocols.47 Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with 200 ng total endotoxin-free plasmid DNA using the Effectene transfection protocol (Qiagen Inc., Valencia, CA, USA). Plasmid DNA encoded for C-terminally EGFP- or DsRed-monomer tagged Panx1b fusion proteins ...
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bioRxiv - Synthetic Biology 2019Quote: ... The final domain-insertion library was obtained by purifying plasmids from this cell slurry using a QIAprep Spin Miniprep Kit (Qiagen, Inc.).
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bioRxiv - Synthetic Biology 2019Quote: ... Gibson assembled constructs were transformed into NEB Turbo cells and isolated colonies were purified for plasmid DNA (QIAprep Spin Miniprep Kit, Qiagen #27106). Plasmid sequences were verified with Sanger DNA sequencing (Quintara Biosciences ...
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bioRxiv - Biophysics 2020Quote: ... HEK-hP1cells were co-transfected with 0.2 μg plasmid DNA of paxillin-mApple (generous gift from Michael Davidson) using transfection reagent Effectene (Qiagen, Valencia, CA), and were cultured for 24 hours.
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bioRxiv - Microbiology 2021Quote: ... reactions to evaluate plasmid copy number were performed using the Rotor-Gene SYBR Green PCR Kit according to manufacturer’s instructions (Qiagen, Hilden, Germany). For each reaction a total of 50 ng of genomic DNA and 200 nM of each oligonucleotide primer were mixed with the Rotor-Gene PCR SYBR Green mix and the reaction was performed in the Rotor-Gene Q instrument ...
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bioRxiv - Immunology 2021Quote: ... plasmid DNA was extracted for each sample from a 1 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Biochemistry 2022Quote: ... All PCR products and digested plasmid were separated by agarose gel electrophoresis and extracted from the gel with QIAquick® Gel Extraction Kit (Qiagen). After assembly ...
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bioRxiv - Biochemistry 2022Quote: ... All PCR products and digested plasmid were separated by agarose gel electrophoresis and extracted from the gel with QIAquick® Gel Extraction Kit (Qiagen). After assembly ...
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bioRxiv - Genomics 2022Quote: ... Following confirmation of the insert sequence the same bacterial colony was used to grow cultures for Maxi-prep which was performed using the EndoFree® Plasmid Maxi Kit (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA was transfected into each well using Effectene (4 μL of enhancer and 5 μL of Effectene reagent; Qiagen 301427). Unless otherwise noted ...
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bioRxiv - Synthetic Biology 2024Quote: ... of Gibson Assembly reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Synthetic Biology 2024Quote: ... of T4 ligation reaction mix was added to NEB Stbl cell (C3040) for transformation and grown at 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA containing RNA–DNA hybrids was isolated from HeLa cells transfected with a pFlag-IN codon-optimized plasmid using a QIAmp DNA Mini Kit (Qiagen, 51304). gDNA was sonicated for 10 min (Diagenode Bioruptor ...
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bioRxiv - Genomics 2023Quote: ... and grown O/N in 4L LB-Amp (Luria-Bertani medium plus ampicillin, 100 µg/ml) and purified using Plasmid Plus Giga Kit (Qiagen, #12991). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 3 × 105 cells.well−1 in 6-well plates and transfected 24 h later with one pmiRGLO derivative construct per well (0.9 µg plasmid) using Effectene (Qiagen cat. #301425). After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... Cleavage products were separated on a 1% agarose gel and the linearised plasmid was purified with a QIAquick Gel Extraction Kit (Qiagen, #28706). Complementary synthetic DNA oligos coding for miR-34a target sites (Table S1) ...
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bioRxiv - Developmental Biology 2024Quote: ... the expression vector reported previously (Macfarlan et al., 2012) was isolated from a bacterial extract using the EndoFree Plasmid Maxi Kit (Qiagen #12362), digested with SacII for linearization ...
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bioRxiv - Systems Biology 2023Quote: ... followed by conventional colony picking and bacterial culture expansion for subsequent plasmid DNA extraction with the Maxi Plus kit from Qiagen (#12964). Next ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Genomics 2024Quote: ... Two independent reactions with 10ng of plasmid subjected to 25 PCR cycles were pooled and cleaned using the PCR clean up kit (Qiagen; 28104). The mutagenesis primer (Supplementary Table 1 ...
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Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approachbioRxiv - Synthetic Biology 2024Quote: ... A total of ∼7×104 individual colonies were then pooled and the plasmid library DNA was isolated by midi-prep kit (Qiagen, UK). 10 μg of the resulting library DNA was then transformed into P ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection was conducted with 5 μg plasmids (act-GAL4, UAS-lbm-GFP, UAS-upd2-mCherry) using Effectene Transfection Reagent (QIAGEN, #301425).
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR array for human cell cycle (Cat#PAHS-020Z) and oncogenes and tumor suppressor genes (Cat#PAHS-502Z) was purchased from Qiagen (Valencia, CA, USA), and was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... To profile the expression of genes related to p53-mediated signal transduction based on the RT² Profiler™ PCR Array Human p53 Signaling Pathway (#330231, PAHS-027ZC, Qiagen, Hilden, Germany), reverse transcription and qPCR were performed using the RT2 First Strand Kit (#330401 ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colonies that grown on the LB-antibiotic plate were picked and inoculated into fresh LB-antibiotic medium at 37 °C to prepare overnight culture for plasmids extraction using QIAprep Spin Miniprep kit (Qiagen, Hilden, Germany). The plasmids were then sent for DNA sequencing (1st BASE ...
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bioRxiv - Microbiology 2019Quote: ... The resulting 11.4kb HIV vector-containing fragments free of plasmid backbone were purified from agarose using QIAquick Gel Extraction Kit (Cat No./ID: 28706 Qiagen, Germantown, MD). A 304 bp zip code-containing insert fragment pool was generated by PCR using NL4-3 GPP or GPV- as template ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid backbones were double-digested with the necessary restriction enzymes and purified using the Qiagen DNA purification kit (#28704X4, QIAGEN GmbH, Germany). DNA inserts were cloned into the digested vectors using the NEBuilder Hifi DNA Assembly Mastermix (#E2621L ...
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bioRxiv - Genomics 2022Quote: ... At least 3 positive colonies per L1 were chosen for Miniprep culture and plasmid DNA was isolated using a QIAprep Spin Miniprep Kit (Qiagen, Cat#: 27106). At least three clones per element were capillary sequenced and compared to identify PCR-induced mutations ...
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bioRxiv - Genomics 2021Quote: ... Confirmed plasmids were cultured overnight in 50mL of luria broth and then DNA extracted using the QIAGEN Plasmid Plus Midi kit (Qiagen cat # 12945).
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bioRxiv - Genomics 2021Quote: ... Several colonies were cultured overnight in 5mL of luria broth and plasmid DNA was extracted using the QIAprep Spin MiniPrep kit (Qiagen cat # 27106). Plasmid DNA was sent for sequencing at the Australian Genome Research Facility (AGRF ...