Labshake search
Citations for Qiagen :
1601 - 1650 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Five colonies with the correct sized insert were inoculated into liquid LB supplemented with spectinomycin and chloramphenicol and grown overnight at 37°C for plasmid extraction using a QIAprep Spin Miniprep Kit (Qiagen). The plasmid constructs were confirmed by Sanger sequencing ...
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bioRxiv - Genomics 2020Quote: ... and a library representation of >200 colonies per guide was determined prior to CRISPR library pool plasmid purification by Endofree Maxi (Qiagen). Coverage of the 818 guide library for Perturb-CITE-Seq as well as the extended control library was confirmed by PCR amplification of the guide sequence from the final library pool and deep sequencing on an Illumina MiSeq.
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bioRxiv - Genetics 2019Quote: ... After 16 hrs in a 37°C shaker bacteria were harvested and plasmid DNA was isolated using a Megaprep kit (Qiagen). ITR integrity was confirmed by digestion with XmaI as well as with AhdI ...
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bioRxiv - Microbiology 2019Quote: ... Genes were predicted using Prokka (78) and plasmids were annotated and visualized via CLC Sequence Viewer (v8.0.0) (Qiagen, Aarhus, Denmark). For clade-to-clade chromosome comparisons ...
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bioRxiv - Genetics 2020Quote: ... Final CRE-reporter plasmids were isolated using Qiagen miniprep columns and were further purified on a Qiagen PCR purification column (Qiagen), and diluted to 50 ng/ml with nuclease free water ...
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bioRxiv - Bioengineering 2020Quote: Plasmid isolation from yeast was performed according to a user-developed protocol from Michael Jones (protocol PR04, Isolation of plasmid DNA from yeast, QIAGEN) using a QIAprep Spin Miniprep kit (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting lawns were scraped off the agar plates and the plasmid libraries were extracted using a QIAprep Spin Miniprep Kit (QIAGEN). The plasmid libraries were subsequently used for yeast transformation.
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA). A minimum of 20 clones from each sample were sequenced ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were subsequently cloned into pGEM®-T Easy Vector plasmids in a JM109 High- Efficiency Competent Cells (Qiagen) strain of Escherichia coli ...
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bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
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bioRxiv - Molecular Biology 2020Quote: ... The integration of plasmids into the endogenous loci was verified by PCR on genomic DNA extracted using the DNeasy Blood & Tissue Kit (Qiagen).
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bioRxiv - Plant Biology 2020Quote: ... Single colonies were used for inoculation of overnight culture and plasmids isolated with the help of the QIAprep Spin Miniprep Kit (Qiagen). The presence of the mutation was verified by Sanger sequencing (LGC genomics) ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega ...
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bioRxiv - Cell Biology 2021Quote: Transgenic parasites were generated by electroporation of sorbitol-synchronized ring-stage parasites with 50-100 μg of purified plasmid DNA (Qiagen). To select for the plasmids pArl-PfCentrin1-GFP and p3-NLS-L3-mCherry ...
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bioRxiv - Cancer Biology 2020Quote: ... mito-roGFP and Myc-epitope-tagged murine Serpin E13,22,23 were purified with Plasmid Plus Midi columns (Qiagen, Inc., Germantown, MD) and administered to 6–8-week-old FVB mice via HDTVI (10 μg each ...
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bioRxiv - Bioengineering 2022Quote: DNA templates for generating desired RNA products were created by PCR amplification from plasmids or gBlock gene fragments (IDT) and purified using a PCR purification kit (Qiagen). Plasmids were then generated with these templates containing a T7 promoter followed by 5’ ribozyme sequence ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant protein fragments were produced in Escherichia coli (strain M15) as fusion products with an N-terminal tag of six histidines using plasmid pQE30 (Qiagen) and purified from total bacterial lysates by nickel affinity chromatography ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). All plasmids were verified for correct size by digest ...
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bioRxiv - Microbiology 2021Quote: ... Resulting colonies were expanded in LB broth with ampicillin and plasmid was isolated using the QIAprep Spin Miniprep Kit (Qiagen). All constructs were confirmed by sequencing.
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bioRxiv - Neuroscience 2020Quote: ... GluA1 or GluA2Q (pIRES2-mCherry or pIRES2-EGFP) and CNIH2 (pRK5 or pBOS) plasmids were transfected at a 1:2 ratio using Effectene (QIAGEN) into adherent HEK293T cells (ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... The CYP168A1 optimized cDNA insert was removed from the plasmid using NdeI and HindIII restriction enzymes and isolated using agarose gel electrophoresis and the Qiagen Gel Extraction Kit (Qiagen) for ligation into a similarly digested and isolated pCWOri+ CYP expression vector (113) ...
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from a wild type clone and a mutant using a QIAGEN Plasmid Midi Kit (Qiagen, Tokyo, Japan) and used as standard DNA to determine ratios of mosaicism ...
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bioRxiv - Genetics 2022Quote: ... 3-5 positive colonies per L1 element were chosen for Miniprep culture and plasmid DNA was isolated using QIAprep Spin Miniprep Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 [60] and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2-3 mL of each bacterial culture was pelleted and plasmid DNA extracted and purified using the QIAPrep SpinPrep miniprep kit (QIAGEN) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... A midi prep scale of plasmid DNA (pGO10GG, 5500 bp, sequence available upon request) was purified following manufacture protocol (Qiagen). The promoter template for in vitro transcription with the human Pol II system was prepared from the native human HSPA1B gene ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was transcribed from a linearized plasmid DNA template using the SP6-Scribe Standard RNA IVT Kit (CELLSCRIPT) and purified with the RNeasy Mini Kit (QIAGEN). The m7G cap structure was added to the purified RNA using the ScriptCap m7G Capping System (CELLSCRIPT) ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant plasmid DNA was linearized with PstI restriction enzyme and purified with a QIAquick PCR Purification Kit (QIAGEN, Germany). Purified template was in vitro transcribed by RiboMAX™ Large Scale RNA Production System with T7 RNA polymerase (Promega ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmid coding for FLP recombinase and the mCherry reporter or 47bp-insertion TLR reporter plasmid were transfected into the cells at a 9:1 ratio using polyfect (QIAGEN) according to the manufacturer’s protocol (900ng mCherry reporter or 47bp-insertion TLR reporter plasmid and 100ng FLP recombinase plasmid to make 1 μg plasmid in total) ...
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bioRxiv - Genomics 2020Quote: ... Both the linearized plasmid and the DNMT3L PCR amplicon were run on an agarose gel and purified in a silica column (Qiagen). Purified fragments were mixed at 1:3 molar ratio and assembled with NEBuilder HiFi DNA Assembly Mater Mix (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). For chlamydial transformation ...
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bioRxiv - Biochemistry 2019Quote: ... which include the Illumina adapters and the 151 bp of plasmid DNA that flanked the mismatch region were purified using the QIAquick PCR Purification Kit (Qiagen) and quantified with PicoGreen (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: pCVB3-H3-WT and pCVB3-H3-VP1-F106L plasmids were linearized with ClaI and purified with the QIAquick Gel Extraction kit (Qiagen). In vitro transcription was performed with the P1230 kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Positive clones were verified by Sanger sequencing analysis and the plasmid containing the chimeric vector was purified with a QIAprep Spin Miniprep kit (Qiagen). The chimeric vector containing the f88-4 epitope 4 (aa 662–671 ...
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bioRxiv - Microbiology 2021Quote: ... coli giant spheroplasts we used a N-terminally 6-His tagged version of TcMscS cloned into the expression plasmid pQE80 (Qiagen) with restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2020Quote: ... and plasmids were subsequently isolated from individual colonies grown overnight in LB broth by using a mini-prep kit (Qiagen). For chlamydial transformation ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...
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bioRxiv - Genomics 2022Quote: ... Wolbachia density relative to host DNA and plasmid copy number were measured by qPCR using the QuantiNova SYBR Green PCR kit (Qiagen) in 10 μL reactions ...
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bioRxiv - Microbiology 2022Quote: ... Biomass was collected by centrifuging at 5,000 rpm for 30 min and fosmid DNA was extracted from the pellet using the QIAGEN Plasmid Midi Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with either pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). For cycloheximide experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transiently transfected with pAc-brm-FLAG-6xHIS in combination with pAc-clk-V5-HIS or pAc-V5-HIS empty plasmid using Effectene (Qiagen). Cells were harvested 48 hours after transfection for processing ...
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bioRxiv - Synthetic Biology 2023Quote: The pARP3 plasmid for methylation analysis was extracted from 200 mL of an overnight culture of Tol 5 transformant cells using the HiSpeed Plasmid Midi kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from five 10-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from ten 4-mL aliquots of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Physiology 2024Quote: ... and NR4A3-overexpressing (NR-203Oex) plasmid DNA was then purified using endotoxin-free buffers (Cat. No. 19048) and columns (Cat. No. 10083) from Qiagen, before assessing DNA concentration and purity by spectrophotometry.
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bioRxiv - Cancer Biology 2024Quote: ... and the p5′UTR-ATF4-FLuc (WT ATF4-FLuc) or p5′UTR-ATF4 A235G-FLuc (mut ATF4-FLuc) plasmids using a Transfection reagent kit (Qiagen) as we previously described10 ...