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Citations for Qiagen :
1501 - 1550 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Cell Biology 2022Quote: ... PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN): control Myc ...
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bioRxiv - Immunology 2022Quote: ... Plasmid extraction was carried out with Qiagen QIApep® spin miniprep kit following manufacturer’s instructions (QIAGEN Ltd, UK). Inserts were confirmed by EcoRI restriction digestion and gel electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... coli grown for 16 h in 250 ml of 2×YT medium using the Plasmid Maxi Kit (QIAGEN) in accordance with the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Glycerol was added to the remaining culture to a final concentration of 15% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from 5 mL of this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). The remaining 95 mL of culture was combined with 40.7 mL 50% glycerol (v/v) ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was isolated using either QIAGEN Plasmid Plus Midi Kit or 28 QiaPrep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Developmental Biology 2024Quote: ... Inserts were PCR amplified using M13 primers and template plasmid was digested with DpnI before PCR purification (Qiagen). Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega) ...
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300g and plasmid DNA isolated using a midiprep kit according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: ... The plasmid DNAs were linearized by a restriction enzyme and purified with the Qiaquick Gel Extraction Kit (Qiagen) before microinjection ...
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bioRxiv - Microbiology 2024Quote: The isolation of DNA (GeneEluteTM Bacterial Genomic DNA, Sigmal-Aldrich) or plasmids (QIAprep® Spin Miniprep Kit, Qiagen) was performed applying commercial kits ...
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were prepared by mini or maxi preps using commercially available kits (Qiagen, UK and Promega, UK, respectively) following manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... All plasmids were purified with a Midiprep kit (ZymoPURE, #D413) or a QIAprep spin miniprep kit (QIAGEN, #27104) and verified by DNA sequencing prior to use ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Biophysics 2023Quote: ... we isolated cosmid-i95 or pBS-T7A1-parS via a midiprep using a Qiafilater plasmid midi kit (Qiagen). We digested the cosmid-i95 for 2 h at 37°C using SpeI-HF restriction enzyme (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids containing the full-length reverse transcriptase gene were isolated with the QIAprep Spin Miniprep kit (Qiagen). Primers ABR-RT-OF and ABR-RT-OR2 were used to sequence the 5’ and 3’ ends of reverse transcriptase ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were isolated from the inoculum and the m9sa cultures following selection using a miniprep kit (Qiagen, Inc.).
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bioRxiv - Biophysics 2023Quote: MCF10A-ER-src cells were transiently transfected with the CAV1 overexpressing plasmid using Effectene transfection reagent (301425, Qiagen). One day before transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen) and the cloned rDNA promoter region was sequenced using Sanger sequencing ...
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bioRxiv - Plant Biology 2023Quote: All plasmid DNA isolation from bacterial cells was performed using the QIAprep Spin Miniprep Kit (Qiagen; Hilden, Germany) according to the manufacturer’s information ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2023Quote: ... coli and the plasmid DNA was extracted using the pipetting Bio Robot Universal System (Qiagen cat. no. 9001094) and the QIAprep 96 plus BioRobot kit (Qiagen cat ...
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bioRxiv - Developmental Biology 2024Quote: ... 200 ng psiCHECK-2 reporter plasmid were co-transfected with 5 µl miScript miRNA mimics (Qiagen; CatNo. 219600) using 4 µl Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The DNA was purified using an endotoxin-free plasmid MaxiPrep kit according to the manufacturer’s instructions (Qiagen 12362). Newly created constructs were sequenced with Eton sequencing (all constructs ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was isolated using alkaline lysis and silica columns using Qiagen miniprep kits (Qiagen Inc, Germantown MD) following the manufacturer’s guidelines.
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... The cDNA libraries were amplified in DH10B E.coli cells and purified using QIAGEN Plasmid Mega Purification kit (Qiagen, Germany). The average size of the cDNA inserts into pPR3-N was evaluated by purifying 20 random colonies each and digesting with SfiI ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid DNA was then extracted with a custom method using reagents from the QIAprep Miniprep Kit (Qiagen cat. #27104) on an automated liquid handler equipped with a positive-pressure filter press.
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bioRxiv - Microbiology 2019Quote: ... pMo-EGFP-PV-WT plasmid was linearized with ApaI and purified with QIAEX II Gel Extraction Kit (Qiagen, Netherlands). With the linearized plasmid as the template ...
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bioRxiv - Biochemistry 2020Quote: ... The selected plasmids were purified by use of a QIAprep Spin Miniprep Kit according to the manufacturer’s instructions (QIAGEN). For the reaction products stemming from transcription with natural CTP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used the remainder of the culture for extracting the plasmid library using a QIAprep Spin Miniprep kit (QIAGEN). The plasmid libraries thus isolated served as templates for the next round of mutagenesis.
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bioRxiv - Immunology 2021Quote: Bacterial plasmid DNA was extracted from a 3 ml overnight culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Next ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were assembled via Gibson Assembly using standard protocols and isolated with the Qiaprep Spin Miniprep kit (Qiagen, USA) (146).
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... All selected colonies were cultured overnight and prepared using the HiSpeed Plasmid Maxi Kit (Qiagen, Hilden Germany, cat# 12663) and sequenced at Genewiz (South Plainfield NJ USA ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA was isolated from select transformants using a miniprep kit according to the instructions of the manufacturer (QIAGEN), and the recombinant construct ...
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bioRxiv - Neuroscience 2020Quote: ... Endotoxin free plasmid preparations of high yield and purity for electroporation was prepared by maxi-prep (Qiagen, Hilden, Germany).
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bioRxiv - Synthetic Biology 2019Quote: ... or Golden Gate Assembly using standard protocols37 Plasmids were routinely isolated using the Qiaprep Spin Miniprep kit (Qiagen, USA), and all primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Genetics 2021Quote: The pEGFP-attB vector with the priEE fragment was prepared at a high concentration using Plasmid Midi Kit (Qiagen) and transported to WellGenetics (Taiwan ...
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bioRxiv - Biochemistry 2020Quote: ... was PCR amplified from plasmid (pSH515) containing the native His4 gene promoter and purified by PCR cleanup kit (Qiagen). DNA was eluted from the spin column with 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: 1×106 MIA PaCa-2 or HAP1 cells were seeded per 6 well plate and co-transfected with 0.5 µg pCas-Guide plasmid using Effectene transfection reagent (Qiagen). 24 hrs after transfection ...
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bioRxiv - Genetics 2020Quote: ... The sgRNA plasmid was linearized with DraI and then purified using a MinElute PCR Purification Kit (Qiagen, Duesseldorf, Germany). sgRNA was produced using the MEGA shortscript Kit (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... Standard molecular biological and microbiological techniques were employed for plasmid DNA extraction (QIAprep Spin Miniprep Kit, QIAgen, Venlo, Netherlands), confirmation of Cys residue position via plasmid-based sequencing (Eurofins Genomics ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were next transfected with pMMP vectors together with retrovirus packaging plasmids using Effectene transfection reagents (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Cells (200,000 for HeLa and hiPSC-CMs and 500,000 for NRCMs) were seeded on glass cover slips and transfected with sensor plasmids using Effectene (Qiagen) (HeLa ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Immunology 2020Quote: ... Freestyle 293F (ThermoFisherScientific, Cat#R79007) cell was transfected with purified miniprep plasmid DNA (QIAgen minipreps kit, Qiagen, Cat#29107) as described (Lipofectamine™ RNAiMAX Transfection Reagent ...
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bioRxiv - Systems Biology 2022Quote: ... the bacteria cells were scraped and centrifuged at 3,000g for 10 min and the plasmids were then extracted (Qiagen). Cloning of individual sgRNA base editor construct was based on similar procedure with reduced reagent input.