Labshake search
Citations for Qiagen :
1851 - 1900 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... DNA was eluted with 66 µL of 2 mM Tris-HCl pH=7.0 to carry out Solution A purification or eluted with 66 µL of 0.1 X EB buffer (From QIAGEN Plasmid Plus Midi Kit) without further Solution A purification.
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bioRxiv - Molecular Biology 2024Quote: ... Liquid cultures were grown at 37°C overnight to saturation and DNA was extracted using QIAGEN® Plasmid Plus Midi Kit (Qiagen cat#12945)
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bioRxiv - Cancer Biology 2021Quote: ... Chemokine expression was quantified using RT2 Profiler PCR array for mouse chemokines/cytokines (Qiagen PAMM-150ZA, 330231) with data analysis performed using the Qiagen online tool PCR Array data analysis Web portal (https://www.qiagen.com/gb/resources/resourcedetail?id=20762fd2-8d75-4dbe-9f90-0b1bf8a7746b&lang=en) ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was extracted from STHdh cells and mouse striatal tissues by using the RNeasy extraction kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... as annotated in miRBase Release 16 (www.mirbase.org) was then performed using miScript Mouse miRNome PCR array (V16.0, 96-well/Rotor-Disc 100; Qiagen) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from mouse lung tissue according to the RNeasy Plus with gDNA removal protocol (QIAGEN) and quantified using an HS RNA kit and Fragment Analyzer (Advanced Analytical) ...
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bioRxiv - Molecular Biology 2022Quote: DNA of the mouse fecal microbiota was isolated by using QIAamp Fast DNA Stool Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from 3D cultures of mouse primary keratinocytes using RNeasy Micro Kit (QIAGEN, UK), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: Total DNA was extracted from mouse fecal samples using QIAamp DNeasy Blood and Tissue Kit (Qiagen, UK) according to the manufacturer’s protocol and as previously described57 ...
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bioRxiv - Pathology 2022Quote: DNA was extracted from mouse tails using the DNeasy® Blood & Tissue Kit (Qiagen®, Hilden, Germany) and DNA concentration was measured with NanoDrop ND-1000 (Thermo-Fisher Scientific Inc.) ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from AML12 cells and mouse livers using RNeasy Mini Kit (Qiagen, Venlo, Nederland) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from the mouse lenses using the RNeasy Plus Micro Kit (QIAGEN, Venlo, Netherlands) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: 500ng mouse or 300ng sheep RNA was reverse transcribed using QuantiTect Reverse transcription kit (Qiagen, Manchester, UK) and gDNA wipe-out (to remove genomic DNA) ...
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 myotubes: Total RNA was extracted using TRIzol and purified using RNeasy Mini spin columns (QIAGEN). Reverse transcription was performed using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Neuroscience 2022Quote: RNA purification from both IPSC and primary mouse cortical cultures were done using RNeasy 96 Kit (Qiagen) according to the manufacturer protocol ...
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bioRxiv - Pathology 2022Quote: ... and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen). The isolated RNA was transcribed to complementary DNA via Superscript IV VILO Master Mix (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bands were transferred to a nitrocellulose membrane and probed with primary antibodies [mouse anti-His (Qiagen) to detect CAT ...
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bioRxiv - Microbiology 2024Quote: Eukaryotic RNA was isolated from mouse tissue samples (stomach and colon) stored in RNAprotect Tissue Reagent (Qiagen) at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from mouse tissue samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... and reads were aligned to the mouse reference genome Refseq GRCm39.105 from the Biomedical Genomics Analysis Plugin 20.0.1 (Qiagen). Normalization of RNA-seq data was performed using trimmed mean of M-values ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from transfected 293T cells and frozen mouse testes using RNeasy Mini kit (QIAGEN). Extracted RNA was used for cDNA synthesis using iScript cDNA Synthesis kit (BIO-RAD ...
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bioRxiv - Biochemistry 2023Quote: Genomic DNA (i.e., gDNA) was isolated from mouse liver tissue using the DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from one quarter of a mouse kidney using RNeasy Plus Mini Kit (Qiagen, 74134). For quantitative real-time PCR ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from mouse cecal contents and in vitro cultures using the RNeasy PowerMicrobiome Kit (Qiagen). Ribosomal RNA depletion was performed using the RiboMinus™ Transcriptome Isolation Kit (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: Total RNA from mouse hearts (normal, ischemic, or non-ischemic) was isolated using RNeasy Mini kits (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was done on mouse kidney lysate using the RNeasy Plus Universal Midi kit (Qiagen). RNase digestion was then performed on the RNA extract using the following RNases ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from de-identified tubes of mouse tissues using the RNeasy Kit (QIAGEN; Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
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bioRxiv - Microbiology 2023Quote: Mouse cDNA was amplified with gene-specific primers (see below) and a HotStarTaq Master Mix Kit (Qiagen). Amplicons were cloned and inserted into a pGEM-T vector (Promega ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary hippocampal neurons grown on coverslips were transiently transfected at DIV11 with Alexa 488-labeled siRNA either specifically targeting mouse Kcc2b (Mm_Slc12a5_1 FlexiTube siRNA, GeneGlobe ID: SI01419243, GenBank accession no: NM_020333; Qiagen) or Ank3 mRNA (Mm_Ank3_3 FlexiTube siRNA ...
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bioRxiv - Microbiology 2024Quote: ... His-tagged proteins were detected with a mouse anti-His HRP-conjugated antibody (1:3000) from Qiagen. MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Cell Biology 2024Quote: ... genetic DNA was isolated from the mouse tails using a DNA isolation kit (QIAGEN DNA isolation kit). Genotyping of Hif2α mice was determined by PCR amplification ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA isolation from isolated hepatocytes and mouse liver was done with RNeasy mini kit (Qiagen, #74106) combined with TRIzol (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163; Qiagen, Redwood City, CA, USA). Lentivirus particles were produced in HEK293T cells after co-transfection of lentivirus plasmid vectors with packaging plasmids (VSVG and lenti gag/pol ...
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bioRxiv - Cancer Biology 2021Quote: Mouse-derived xenograft HCC tumors were homogenized in RIPA buffer using metal beads and the LT TissueLyser (Qiagen). Samples were sonicated (Branson Sonifier 3310s ultrasound probe ...
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bioRxiv - Microbiology 2022Quote: Total genomic DNA of mouse fecal samples was extracted using QIAamp PowerFecal Pro DNA Kit (Qiagen, CAT#: 51804), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Plcg2 knockdown was achieved in M-MØP cells using an antisense LNA GapmeR produced against mouse Plcg2 (Qiagen). Cells were grown to 80% confluence (∼2.4 × 105 cells per well ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA from mouse bone marrow cells was extracted by the DNeasy Blood and Tissue Kit (Qiagen, #69504). The DNA region containing sgRNA target sites was amplified by PCR using specific primers (Zrsr2 Forward ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10 µL of mouse sera using QIAamp® viral RNA Mini Kit (Qiagen) or about 20 mg of organs homogenised by Precellys Lysing Kit Hard tissue grinding (Bertin Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cell-free cfDNA (both human and mouse) was isolated with QIAamp DSP Circulation NA Kit (QIAGEN, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nucleic acids were extracted from mouse fecal samples and inoculum samples using the DNeasy Powersoil HTP Kit (QIAGEN) and from the further simplified SC2 samples using the Powermag Microbiome kit (MoBio) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: DNA from mouse heart ventricular tissues or NRVMs was extracted using QIAamp® DNA Mini Kit (Qiagen, 51304) according to the manufacture’s procotol ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was analyzed with the Qiagen RT2 Mouse Cytokines and Chemokines array (PAMM-150Z, Qiagen, Hilden, Germany). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133 ...