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Citations for Qiagen :
1651 - 1700 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 250 ng of the plasmids with the intronic fragments were expressed into the S2 cells with Effectene Transfection Reagent from Qiagen. For dFOXO ...
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bioRxiv - Synthetic Biology 2023Quote: ... Genomic DNA and plasmids were extracted using the Fast Pure Bacteria DNA isolation mini kit (Vazyme) and the QIAprep Spin Miniprep Kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Biophysics 2022Quote: ... The AbundancePCA plasmid and BindingPCA plasmids were all digested by HindIII and NheI enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN). The assembly of AbundancePCA libraries and BindingPCA libraries were done overnight by temperature-cycle ligation using T4 ligase (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... were used as per manufacturer’s instructions to clean up the reacted plasmid DNA and eluted in a 50 μL volume in buffer EB (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were selected from transformation plates and liquid bacterial cultures grown as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Neuroscience 2023Quote: ... and colonies yielding an ≈2.4 kB product were isolated from a duplicate patch plate and grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Biophysics 2023Quote: ... The following day we performed three more rounds of plasmid clean-up using the same QIAprep Spin Miniprep kit (Qiagen) to remove all the residual enzymes which could corrupt the AFM images.
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bioRxiv - Biophysics 2023Quote: ... We grew sequence-positive clones for the plasmid extraction at 37°C overnight in presence of a selective antibiotic Chloramphenicol (30 µg/ml) and isolated the final plasmid using a QIAprep Spin Miniprep kit (Qiagen) the following day ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding the recombinant RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). On Day 0 ...
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bioRxiv - Biophysics 2023Quote: ... WT and mutant DNA plasmids were linearized by HindIII-HF or EcoRI-HF and purified with the QIAquick PCR Purification Kit (QIAGEN). cRNA was transcribed in vitro with the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Genetics 2022Quote: ... The linearized plasmid backbones and PCR products were analysed on agarose gels and purified using the QIAquick gel extraction kit (Qiagen). Assembly of DNA fragments was either performed with isothermal assembly or Goldengate-like reactions (see details on individual plasmids below) ...
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bioRxiv - Biochemistry 2023Quote: ... Up to three resistant colonies per mutation were selected and plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen) and quantified using a nanodrop spectrophotometer (ND.1000) ...
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bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA from overnight liquid cultures (LB with 100 mg/ mL ampicillin) was isolated with QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Developmental Biology 2023Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 (Klueg et al., 2002) and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were subsequently purified via overnight culture followed by purification via a commercial endotoxin-free spin midi prep kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Neuroscience 2023Quote: ... primary hippocampal neurons were transfected in duplicates or triplicates using 150ng of a plasmid carrying enhanced GFP-gene in combination with 5nmol Power Lock-Nucleic Acid inhibitors (Qiagen) against miR-218-5p ...
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bioRxiv - Microbiology 2023Quote: ... pHIVec2.luc reporter plasmid and psPAX2 packaging plasmid (catalog number 11348, NIH AIDS Reagent Program) in a ratio of 1:6:3 using either Effectene (Qiagen) or calcium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 0.35 μg of psPAX2 or pNL(AD8) and 0.45 μg of a reporter plasmid using Effectene transfection reagent (Qiagen, USA). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... shaking overnight at 30°C for 16hrs and the plasmid DNA is purified using a QiaPrep 96 column DNA purification kit (Qiagen). This procedure was conducted twice on different days to produce two separate replicates.
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bioRxiv - Cancer Biology 2023Quote: ... NHEJ reporter plasmid was digested with I-Sce1 for 6h and purified using a QIAEX II Gel Extraction Kit (QIAGEN). Exponentially growing cells were transfected using an Amaxa nucleofector with the U-023 program ...
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bioRxiv - Biophysics 2024Quote: ... Mutant cDNAs were then verified by Sanger sequencing (Eurofins Genomics) and purified on larger scale using a HiSpeed Plasmid Midi Kit (Qiagen).
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bioRxiv - Bioengineering 2024Quote: ... kanamycin-positive colonies were selected from the agar plate and plasmids were extracted using the MiniPrep isolation kit (Qiagen, USA). The cloned construct was eventually confirmed using Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: De novo reconstruction of plasmid sequences was performed by first isolating high molecular weight DNA with the Qiagen Genomic-tip 20/G protocol (Qiagen). Libraries were constructed using the Rapid Barcoding kit (Oxford Nanopore Technologies (ONT) ...
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bioRxiv - Biochemistry 2024Quote: ... After cutting 20 µg of plasmid DNA with a NotI-HF (New England Bioscience) and purification using the QIAquick PCR purification kit (Qiagen), 4×107 Tb427sm cells were washed once in ice-cold cytomix (2 mM EDTA ...
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bioRxiv - Plant Biology 2024Quote: ... All plasmid clones were prepared for sequence analysis using the QIAGEN QIAprep kit according to the instructions of the supplier (QIAGEN). From pDONR221 clones ...
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bioRxiv - Neuroscience 2021Quote: Mouse posterior cortex was disrupted for RNA isolation using the TissueLyser (QIAGEN, Hilden, Germany) for 2 x 2 min at 20 Hz as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: Purified RNA was isolated from mouse skin using the RNeasy Plus Mini Kit (Qiagen) after pulverization of the frozen skin tissue and homogenization with the QIAshredder system (Qiagen) ...
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bioRxiv - Biochemistry 2019Quote: Fecal DNA from mouse colon was extracted by QIAamp PowerFecal DNA Kit (Qiagen, Germany) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from mouse hippocampal samples using a RNeasy Mini kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and from mouse tissue (liver, retina, RPE) using a DNeasy Blood & Tissue kit (Qiagen). Target sequences were PCR-amplified using 2X Taq PCR Smart mix (SolGent) ...
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bioRxiv - Physiology 2020Quote: Whole RNA was extracted from mouse orbital fat samples using RNeasy mini-kits (Qiagen). cDNA was prepared using Superscript III first-strand synthesis kit (ThermoFisher) ...
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bioRxiv - Pathology 2022Quote: Total RNAs were obtained from cells or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was subsequently isolated from mouse plasma using the MinElute Virus Kit (Qiagen) on the QiaCube ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was isolated from mouse tissues using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Total RNA from mouse lungs was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: Total RNA (100–200 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA quality and quantity was measured on the Agilent 2200 TapeStation System ...
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bioRxiv - Molecular Biology 2020Quote: Total mouse heart RNA was isolated with an RNeasy mini kit (Qiagen, Valencia, CA) or RNAqueous® Micro RNA isolation kit (Ambion ...
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bioRxiv - Immunology 2019Quote: ... RT2 Profiler™ PCR Array Mouse Autophagy was purchased from Qiagen (Catalog # PAMM-084Z). RNA extracted from the brain of pups born to infected dams were analyzed for the expression of autophagy-related genes following the manufacturer’s instruction and as previously described (13).
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from mouse MLL-AF9 cells by RNeasy Micro Kit (Qiagen), 72 hours after transduction of the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... for mouse samples and cell lines or with miRNeasy Mini Kit (Qiagen, Hilden, Germany) for human tissue samples ...
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bioRxiv - Microbiology 2024Quote: ... spongiae DNA was extracted from mouse tissues using DNAeasy Blood and Tissue kit (Qiagen). The numbers of mice used in each individual experiment were calculated to permit detection of at least a two- to four-fold difference in bacterial loads between groups with 95% (two-sided ...
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bioRxiv - Molecular Biology 2024Quote: RNA was extracted from mouse islets using the RNAeasy Micro Kit (QIAGEN, Cat # 74004); RNA was extracted from flash-frozen liver and peripancreatic fat tissues using TRIzolTM (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: Mouse cDNA was amplified with gene-specific primers and HotStarTaq Master Mix Kit (Qiagen) (Table 1) ...