Labshake search
Citations for Qiagen :
1751 - 1800 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Two independent reactions with 10ng of plasmid subjected to 25 PCR cycles were pooled and cleaned using the PCR clean up kit (Qiagen; 28104). The mutagenesis primer (Supplementary Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... Colony PCR was performed with KOD One™ PCR Master Mix (Toyobo) to screen colonies before recovering plasmids with a 5 mL overnight culture and miniprep (QIAgen).
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from mouse testes using an RNeasy Kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from mouse tail tissue using DNeasy Blood and Tissue Kits (Qiagen) following digestion with proteinase K ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from frozen mouse skin tissues using the RNeasy Plus kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was purified from laser-microdissected mouse SCN using the RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 100 ng/well of mouse anti-Penta His BSA-free antibody (Qiagen) in PBS ...
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bioRxiv - Physiology 2021Quote: Total RNAs were obtained from human UREC or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using SuperScript II Reverse Transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from mouse urothelium was obtained using the RNeasy mini kit (Qiagen, Courtaboeuf, France) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... a 1:2000 dilution of a mouse anti-Penta-His Alexa Fluor 647 conjugate (Qiagen) was used.
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bioRxiv - Cancer Biology 2022Quote: ... genomic DNA was isolated from mouse tails using DNeasy Blood and Tissue Kit (Qiagen, #69506). PCR was carried out using Econo-Tag Plus Green 2x master mix and the following primers ...
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bioRxiv - Neuroscience 2021Quote: RNA from bulk mouse brain tissue was extracted using the RNeasy Plus Mini Kit (Qiagen) and resuspended in nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: Mouse cerebral cortex: Cortical tissue was collected and immediately stored in RNALater Stabilization Solution (Qiagen) at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse anti-StrepII and anti-His antibodies were obtained from QIAGEN (catalog number 34850) and Genscript (catalog number A00186) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from adult mouse hippocampal tissue using an RNeasy Plus Kit (Qiagen). Amplification of 5’ cDNA ends was performed using the GeneRacer™ Kit (Life Technologies ...
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bioRxiv - Physiology 2022Quote: RNA was isolated from cells and mouse kidney using the RNeasy Mini Kit (Qiagen, DE). cDNA was generated using the QuantiTect reverse transcription kit with gDNA Wipeout (Qiagen ...
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bioRxiv - Microbiology 2022Quote: Mouse lungs were homogenized and RNA was extracted using the Qiagen RNeasy Mini Kit (Qiagen). Extracted RNA was used for cDNA synthesis using Superscript III reverse transcriptase (Invitrogen ...
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bioRxiv - Physiology 2019Quote: Total islet RNA (100–200 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA concentration and integrity were assessed using the ND-1000 Spectrophotometer (NanoDrop) ...
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bioRxiv - Microbiology 2019Quote: ... 48 hours post infection urine was collected from each mouse directly into bacterial RNAprotect (Qiagen). All collected urine was pooled together and pelleted ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was extracted from distinct mouse brain regions using the RNeasy Mini Kit (QIAGEN). The QuantiTect Reverse Transcription Kit (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... total mRNA was isolated from mouse and human B cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Microbiology 2019Quote: ... mouse faecal samples were extracted with the QIAamp DNA FAST Stool Mini Kit (QIAGEN, Germany) (Lim et al. ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from mouse skin-punch biopsies using the DNeasy tissue kit (QIAGEN, #69506) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... paraffin-embedded (FFPE) stomach sections per mouse using the AllPrep DNA/RNA FFPE Kit (Qiagen) and gene expression was detected using the nCounter Mouse Immunology Panel (NanoString) ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from mouse retinas or from HRMEC culture with RNeasy Kit (Qiagen) based on manufacturer protocols ...
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bioRxiv - Cell Biology 2021Quote: ... Each biological replicate (mouse) was run in triplicate and 18S ribosomal RNA (Rn18S, Qiagen, QT02448075) was used as a house keeping gene for normalization.
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bioRxiv - Cell Biology 2021Quote: ... adipocytes and mouse adipose tissue RNeasy Lipid Tissue Mini Kit was used (Qiagen, cat. 74804) following manufacturer instruction ...
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bioRxiv - Neuroscience 2022Quote: ... the mouse spinal cord sections were treated with 10 μg/mL proteinase K (QIAGEN, 19131) at 37°C for 5 min and then post-fixed in 4% PFA at RT for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was isolated from frozen mouse whole brains using the RNeasy Midi kit (Qiagen). QRT-PCR was performed according to a two-step process using the Quantitect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from mouse hearts was extracted using QIAGEN’s miRNeasy Mini kit (217004; Qiagen, Germany). The reverse transcription step was performed using Takara’s PrimeScriptTM RT Master Mix (RR036A ...
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bioRxiv - Molecular Biology 2022Quote: RNAs from HUVECs or mouse lung ECs (mLECs) were purified using RNeasy-kit (74106, Qiagen). The RNA was reverse transcribed High-Capacity cDNA Reverse Transcription Kit (4368813 ...
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bioRxiv - Physiology 2024Quote: Total islet RNA (200–300 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA concentration and integrity were assessed using the ND-1000 Spectrophotometer (NanoDrop) ...
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bioRxiv - Neuroscience 2023Quote: RNA from bulk mouse brain tissue was extracted using the RNeasy Plus Mini Kit (Qiagen) and resuspended in nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... Blots were probed overnight using a mouse monoclonal anti-StrepII antibody (Qiagen catalog number 34850) at a 1:2500-1:5000 dilution or a rabbit polyclonal antiserum raised against the PlpD C-terminal peptide NH2-EWLTRVQLGDRQELYSEFYQC-COOH at a 1:5000 dilution ...
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bioRxiv - Pathology 2023Quote: Mouse tissues were homogenized with a 5 mm steel bead using a TissueLyser II (QIAGEN) for 5 min at frequency of 30 times/second ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA from mouse blood was extracted using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from astrocytes of mouse brain using a RNeasy mini kit (Qiagen). For each sample ...
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bioRxiv - Neuroscience 2024Quote: Mouse brain tissues were disrupted and homogenized using QIAzol lysis reagent (Qiagen, Hilden, Germany, 79306), and the Tissue Lyser instrument (Qiagen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: DNAs from mouse cecal contents were extracted using the QIAamp DNA Stool Mini Kit (QIAGEN), including a bead-beating step (0.1 mm zirconia silica beads ...
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bioRxiv - Immunology 2024Quote: RNA was extracted and purified from mouse lungs using the RNeasy plus mini-kit (QIAGEN). A cDNA library was prepared using the Illumina TruSeq stranded mRNA protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Colonies that grown on the LB-antibiotic plate were picked and inoculated into fresh LB-antibiotic medium at 37 °C to prepare overnight culture for plasmids extraction using QIAprep Spin Miniprep kit (Qiagen, Hilden, Germany). The plasmids were then sent for DNA sequencing (1st BASE ...
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bioRxiv - Microbiology 2019Quote: ... The resulting 11.4kb HIV vector-containing fragments free of plasmid backbone were purified from agarose using QIAquick Gel Extraction Kit (Cat No./ID: 28706 Qiagen, Germantown, MD). A 304 bp zip code-containing insert fragment pool was generated by PCR using NL4-3 GPP or GPV- as template ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid backbones were double-digested with the necessary restriction enzymes and purified using the Qiagen DNA purification kit (#28704X4, QIAGEN GmbH, Germany). DNA inserts were cloned into the digested vectors using the NEBuilder Hifi DNA Assembly Mastermix (#E2621L ...
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bioRxiv - Genomics 2022Quote: ... At least 3 positive colonies per L1 were chosen for Miniprep culture and plasmid DNA was isolated using a QIAprep Spin Miniprep Kit (Qiagen, Cat#: 27106). At least three clones per element were capillary sequenced and compared to identify PCR-induced mutations ...
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bioRxiv - Genomics 2021Quote: ... Confirmed plasmids were cultured overnight in 50mL of luria broth and then DNA extracted using the QIAGEN Plasmid Plus Midi kit (Qiagen cat # 12945).
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bioRxiv - Genomics 2021Quote: ... Several colonies were cultured overnight in 5mL of luria broth and plasmid DNA was extracted using the QIAprep Spin MiniPrep kit (Qiagen cat # 27106). Plasmid DNA was sent for sequencing at the Australian Genome Research Facility (AGRF ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA (gDNA) was prepared in a plasmid- and PCR product-free work area using a QIAamp DNA Blood Maxi Kit (Qiagen, Cat. #51192) according to manufacturer instructions and previously published protocols27 ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Bioengineering 2023Quote: ... which were cultivated on a rotary shaker at 37° for 6 h before plasmid isolation using a Midi Kit (Qiagen, Hilden, Germany). The obtained plasmid libraries were subsequently used for electroporation of C ...