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Citations for Qiagen :
1551 - 1600 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... each 50 mL midiprep culture was spun down for 30 minutes at 4,300 x g and plasmid DNA isolated using a midiprep kit according to the manufacturers instructions (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... was used to amplify DNA fragment except for PCR verification of plasmids or strains for which Taq polymerase (Qiagen) was used ...
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bioRxiv - Microbiology 2024Quote: ... The viral RNA product was then treated by DNase to remove the carry-over plasmid DNA (RNeasy Kit, Qiagen). cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded in 24-well plates and transfected with the desired plasmids using Effectene Transfection Reagent (#301427, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cx43 constructs with a stop codon in the eGFP-NI plasmid were purified with QIAprep Midi Prep Kit (Qiagen) suitable for Lipofectamine2000 (Invitrogen/Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were normalised to cell density and DNA was isolated using the Plasmid Maxi Kit (Qiagen, Cat. No. 12163) according to manufacturer’s directions except that all steps and reagents ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-ZMYM2(WT) (pAS4329) or EGFP-ZMYM2(SIMmut) (pAS4330) plasmids using Polyfect Transfection Reagent (Qiagen, Cat. No. ID: 301105) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Colonies were cultured in 2x YT liquid media overnight and plasmids were extracted using QIAprep Spin Miniprep kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... The plasmid was transformed into stbl3 competent E.Coli and DNA was harvested from 200 mL growth media using an endotoxin free plasmid purification kit (Qiagen).
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bioRxiv - Genetics 2023Quote: ... Bacterial cells were cultured overnight in liquid and the final libraries were extracted using QIAGEN Plasmid Maxi Kit (QIAGEN). The libraries were quantified and subpools combined in 1:4.76 molar ratio to get the final library containing 5,760 gRNAs.
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bioRxiv - Cell Biology 2023Quote: ... Overnight cultures were pelleted at 3,000g for 10 min and plasmid DNA was purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Systems Biology 2023Quote: ... The supernatant was recovered and plasmid DNA was purified following the standard QIAprep Spin Miniprep Kit (QIAGEN, Hilden, Germany) protocol and eluted in 50 μl EB buffer.
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bioRxiv - Cancer Biology 2023Quote: ... and 9-10 mL of Buffer BB (1/3 of the lysate volume, From QIAGEN Plasmid Plus Midi Kit) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... Crude eccDNA was then eluted by using 100 µL of 0.1x EB buffer (From QIAGEN Plasmid Plus Midi Kit). For each microgram crude eccDNA we spiked in 1 ng pUC1932 (was a gift from Joachim Messing ...
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bioRxiv - Cell Biology 2023Quote: ... Linearized plasmids were purified using a QIAquick PCR purification kit according to the manufacturer’s instructions (28106, Qiagen, Hilden, Germany). Templates for CLN3 RNA synthesis were in the same manner but using CLN3 specific primers (for ...
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bioRxiv - Biochemistry 2023Quote: ... An endotoxin-free preparation of the cloned vector was made using the EndoFree Plasmid Maxi Kit (Qiagen, Hilden, DE).
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bioRxiv - Biochemistry 2024Quote: ... Overnight cultures were pelleted at 3,000 g for 10 min and plasmid DNA was extracted and purified using a Qiagen miniprep kit (27106, Qiagen). Sequences were verified by Sanger sequencing (Eton Bioscience Inc).
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bioRxiv - Neuroscience 2024Quote: ... 1µg of pMT-FH-clk and 1µg of pMT-ck1α(WT)-6Xc-myc or pMT-ck1α(K49R)-6Xc-myc in combination with 1µg of pCoHygro plasmid expressing hygromycin resistance were used for transfection using Effectene (Qiagen). ck1α(K49R)-6Xc-myc encodes a kinase dead variant of the kinase ...
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bioRxiv - Molecular Biology 2020Quote: ... miRCURY LNA Power Inhibitor against mouse miR-375 (mmu-miR-375-3p) (Qiagen, Hilden ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted from mouse liver samples using RNeasy Mini Kit (Qiagen), DNase treated and 1 μg total RNA was processed for mRNA sequencing ...
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bioRxiv - Neuroscience 2020Quote: Pre-weighed cortex pieces from mouse brains were homogenized in QIAzol (Qiagen 79306), at 1ml volume per 100mg of tissue ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from mouse tissues using QIAzol Lysis Reagent Protocol (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA of mouse islets was extracted using RNeasy Plus Mini Kit (Qiagen). Other tissues including mouse liver ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from mouse right adrenals using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from mouse right adrenals using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA from mouse liver was isolated using RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Genomics 2019Quote: We analyzed human and mouse Tug1 cDNA sequences with CLC Genomics Workbench (Qiagen) for open reading frames (ORFs) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... we prepared total RNA from dissected wildtype mouse brain regions using Qiazol (Qiagen) and prepared cDNA using the SuperScript III first-strand synthesis kit (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from mouse pituitaries using an RNeasy mini kit (Qiagen) and 250 ng converted to cDNA using Revertra Ace (TOYOBO ...
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bioRxiv - Genetics 2020Quote: Mouse tissues were disrupted and homogenized using QIAzol lysis reagent (Qiagen, Hilden, Germany) and the Tissue Lyser instrument (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... or RNeasy Mini Kit (synaptosome and cytosolic fraction from homogenized mouse forebrain; Qiagen). For RT-PCR ...
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bioRxiv - Immunology 2020Quote: RNA from mouse plasma was isolated using Qiamp RNA-mini isolation kit (Qiagen) and RNA from tissues isolated using the RNeasy mini isolation kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from mouse B cells using the DNeasy kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2022Quote: Proteins were extracted from frozen mouse left ventricles using the TissueLyser LT (Qiagen) with 5mm stainless steel beads (69989 ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted from mouse neural retina using RNeasy mini kit (QIAGEN). Total RNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time ...
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bioRxiv - Neuroscience 2024Quote: ... and the mouse hippocampal tissues (n=5-6/group; Qiagen, Germantown, MD, USA). The samples of the RNA (500ng/µL ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2023Quote: ... Total mRNA was extracted from mouse tumors using the RNeasy Mini Kit (QIAGEN) and subsequently tested for concentration and integrity via NanoDrop 2000c (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: We extracted RNA from fresh mouse using an AllPrep DNA/RNA kit (Qiagen). We placed 5-10 mg of tissue into 350 μL RLT Plus Buffer in a 2 mL tube containing a 5mm stainless steel bead and homogenized with a TissueLyzer for two 2 min rounds at 30 Hz ...
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bioRxiv - Physiology 2023Quote: ... total RNA was prepared from mouse liver using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from mouse ESCs with QIAzol lysis reagent (Qiagen #79306), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal antibody that recognizes the strep-tag epitope was purchased from Qiagen. Recombinant NifB expressed in E ...
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bioRxiv - Synthetic Biology 2024Quote: Total RNA was extracted from mouse tissue using RNeasy plus kit (74136, QIAGEN) and Maxtract High-density tubes (129056 ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was extracted from mouse B cells by RNeasy Micro kit (QIAGEN) and converted to cDNA by using PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Neuroscience 2024Quote: ... The RT² Profiler™ PCR Array Mouse Common Cytokine (Qiagen, PAMM-021ZA-12) was used to analyze cytokine gene expression ...
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bioRxiv - Biophysics 2021Quote: ... The mutagenesis plasmid pGJJ055 was digested with HindIII-HF and AvrII and purified with the MinElute PCR Purification Kit (QIAGEN). The GRB2 mutagenesis plasmid (pGJJ057 ...
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bioRxiv - Cell Biology 2019Quote: ... insertion of the gRNA was confirmed by gel electrophoresis of PCR products and the plasmid was purified using the QiaQuick Gel Extraction kit (Qiagen) following provider’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cyp6a8-pOT2 and Cyp6d5-pOT2 plasmids were linearized overnight and purified into RNAse-free water using the MinElute Reaction Cleanup Kit (Qiagen). Labeled S and AS probes were generated using the DIG RNA Labeling Kit (SP6/T7 ...