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Citations for Qiagen :
1401 - 1450 of 2080 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 mL were collected and used for plasmid extraction using a QI-Aprep Spin Miniprep kit (QIAGEN). Extracted plasmids were eluted in 30 μL of elution buffer and stored at −20 °C until use.
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bioRxiv - Developmental Biology 2021Quote: ... plasmid encoding Cas9 and gRNA or WT control organoids using QIAamp Fast DNA Tissue Kit (51404, Qiagen). PCR was performed using PrimeSTAR® GXL DNA Polymerase (R050A ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were isolated from 5 mL of overnight liquid culture using QIAprep Spin Miniprep Kit (Qiagen, Germany) and the presence of mutations was confirmed by Sanger Sequencing.
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bioRxiv - Cell Biology 2021Quote: ... CRISPR/Cas9 and donor plasmids were co-transfected into the cell lines using Effectene (Qiagen, Venlo, Netherlands). For drug selection ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA used in transfection of cells was purified using the Qiagen MaxiPrep kit (Qiagen, Valencia, CA).
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bioRxiv - Bioengineering 2022Quote: The plasmid DNAs were linearized with XbaI digestion and purified with Post PCR cleanup kits from Qiagen. The linearized DNAs were used as a template for in vitro transcription with T7 RiboMAX™ Large-Scale RNA Production System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using the phenol-chloroform protocol combined with Phase Lock Gel tubes (Qiagen GmbH) and was detected by the agarose gel electrophoresis as well as quantified by Qubit® 2.0 (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... After transformation of the cloned vectors and purification of the DNA using EndoFree Plasmid Maxi Kit (Qiagen), the purified products were sent to Microsynth AG (Balgach ...
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bioRxiv - Biochemistry 2021Quote: ... pX330 plasmid DNA expressing the active sgRNA was purified with an endotoxin free kit (Qiagen, Germantown, Maryland). Plasmid DNA concentration was adjusted to 5 ng/ul for pronuclear microinjection (Mashiko et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Random colonies were selected to use in plasmid extraction using QIAprep Miniprep Kit (QIAGEN, Valencia, CA, USA). Positive plasmids were confirmed by PCR reactions ...
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bioRxiv - Developmental Biology 2019Quote: ... The DNA plasmids of ampicillin-resistant bacteria were extracted using the QIAprep Spin Miniprep Kit (Qiagen, 27106) and the sequences confirmed via DNA sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with the Cignal Reporter Plasmids for GRE using a dual luciferase assay system (Qiagen). Appropriate treatment (siGAS5 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid pENTR-CTDSP1 was extracted from positive colonies using the QIAprep Spin Miniprep Kit (Qiagen) and proper orientation of the cloned fragment was verified by PCR and DNA sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were purified from the transformed colonies using the QIAprep Spin Miniprep Kit (Qiagen, Valentia, CA, USA) and the mutated regions were confirmed by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... All plasmids used for in vitro experiments were purified using Midiprep kit including endotoxin removal step (Qiagen). pCMV-PE2 was a gift from David Liu (Addgene plasmid # 132775 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA was isolated from bacterial cells using a commercial QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany). Restriction endonucleases were purchased from New England Biolabs (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant plasmids were extracted from cell pellets using the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA, USA). The sequence of the inserts was confirmed by Sanger sequencing (W ...
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bioRxiv - Immunology 2021Quote: ... Phagemid DNA from the third and fourth rounds of phage selection was isolated (Plasmid Miniprep kit, Qiagen) and the genes encoding scFv fragments were ligated into an in-house constructed screening vector providing the secreted scFv with a triple-FLAG tag and a hexahistidine (His6 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°) and plasmids were extracted from the cell pallets using QIAprep Spin Miniprep kit (Qiagen, CA, USA). The sequences of the inserts were confirmed by Sanger sequencing (ACGT Inc ...
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bioRxiv - Bioengineering 2022Quote: ... The phage-containing pellet was then processed using an Endofree Plasmid Giga Kit (Qiagen, cat. code 12391), with the following adjustments ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with 500ng plasmid DNA and a 1:50 Effectene transfection reagent ratio (Qiagen #301425).
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bioRxiv - Molecular Biology 2023Quote: All purification of plasmid DNA or PCR products was done using commercially available kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids with the desired constructs were generated using an endotoxin free maxiprep kit (12362, QIAGEN, Germantown, MD) and confirmed via whole plasmid NGS ...
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bioRxiv - Cell Biology 2022Quote: ... colonies were scraped off plates and library plasmids purified using a Qiagen HiSpeed Maxi kit (Qiagen, 12662). Plasmids were eluted in Qiagen Buffer TE ...
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bioRxiv - Neuroscience 2024Quote: ... Resulting plasmids were verified with diagnostic restriction digest and Sanger sequencing prior to being maxi-prepped (Qiagen) for subsequent use in excitotoxicity assays.
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bioRxiv - Systems Biology 2024Quote: ... and a frozen aliquot of each was later collected and plasmids were extracted via miniprep kit (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... Purified plasmid DNA was generated from overnight LB cultures with the QIAprep Spin Miniprep Kit (Qiagen, #27106X4) and quantified using the NanoDrop ND-1000 spectrophotometer (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA was isolated from individual clones using the QIAprep Spin miniprep kit (Qiagen, Cat. No. 27106), and the cloned plasmids were subjected to sequencing using T7 forward primer and SP6 reverse primer designed for the vector backbone ...
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bioRxiv - Cell Biology 2024Quote: ... The linearised plasmid was run on agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen; #28704). sgRNA oligonucleotides (TAG Copenhagen AS ...
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bioRxiv - Microbiology 2023Quote: Protein complexes were produced from the relevant pQE70 plasmid (see Table S2) in strain M15 pREP4 (Qiagen) as follows ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid sub-libraries were purified from the bacteria using the QIAamp DNA Mini Preparation Kit (Qiagen). The DNA concentration was measured using the Quant-iT dsDNA (HS ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids were recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen) and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Genetics 2023Quote: ... Plasmid DNA was isolated from the pooled transformant cells using a High-Speed Maxi Prep kit (Qiagen).
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture with Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture using Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration of each vector was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... phagemid DNA was extracted from bacterial culture using the QIAprep spin mini prep plasmid extraction kit (Qiagen) with OD260 value was measured by NanoDropTM2000 (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
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bioRxiv - Systems Biology 2023Quote: ... Cells were harvested by centrifugation and plasmid DNA was isolated using the QIAGEN Midiprep Plus kit (QIAGEN). Plasmid pools ...
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bioRxiv - Developmental Biology 2023Quote: ... The plasmid was linearized by ScaI restriction digest and purified using the QIAquick PCR purification kit (Qiagen). Purified linear plasmid was transfected using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: DNA plasmids were isolated from the overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from single colonies was extracted with the QIAprep Spin Miniprep Kit and Maxi Kits (Qiagen) and confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2023Quote: ... All colonies were collected by scraping and the DNA was extracted using the Plasmid maxiprep (Qiagen #12165). For a small proof-of-principle library containing 7 different targets ...
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bioRxiv - Cancer Biology 2023Quote: ... Took off the tube and used 30 µL 0.1X EB buffer (From QIAGEN Plasmid Plus Midi Kit) to resuspend the beads and incubated for more than 3 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The constructs were amplified in OneShot Stabl3 cells and purified using a plasmid midi kit (Qiagen 12945) for high yields of endotoxin-free DNA ...
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bioRxiv - Cancer Biology 2024Quote: All purification of plasmid DNA or PCR products was performed using commercially available kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The colonies were cultured overnight for plasmid DNA isolation using QIAprep Spin Miniprep Kit and Qiacube (Qiagen). Diagnostic digest was performed for confirming the positive clones ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then performed plasmid extractions from the overnight cell cultures using the QIAprep Spin Miniprep Kit (QIAGEN) for plasmid purification ...