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Citations for Qiagen :
1301 - 1350 of 1933 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was harvested from mouse ear skin using TissueLyser II (Qiagen) and TRIzol (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: Mouse RNAs were purified using the Rneasy Plus Mini Kit (Qiagen) and subsequently treated with DNase ...
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bioRxiv - Molecular Biology 2023Quote: ... The mouse aorta was homogenized by TissueLyser II (Qiagen, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RT2 Profiler PCR Array Mouse p53 Signaling Pathway (PAMM-027Z – Qiagen) was used for quantification ...
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bioRxiv - Systems Biology 2020Quote: ... The plasmids were bought in lyophilized form (Arbor Biosciences) and purified using QIAprep Spin Miniprep Kit (Qiagen) using cell lines DH5-Alpha (for P28a-cI-ssrA ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from 50 ml LB from an overnight culture using a plasmid midi kit (Qiagen). Successful genomic DNA extraction was confirmed by PCR with primers gp41 inner F (5’-CAAGAGCAAAGAACCGACG-3’ ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK-293T cells transfected with the desired plasmids by using Attractene Transfection Reagent (301005, QIAGEN, Hilden, Germany), according to the fast-forward protocol ...
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bioRxiv - Genetics 2021Quote: ... All TALE-VP64 and TALE- Δ plasmids were miniprepped using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 mL were collected and used for plasmid extraction using a QI-Aprep Spin Miniprep kit (QIAGEN). Extracted plasmids were eluted in 30 μL of elution buffer and stored at −20 °C until use.
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bioRxiv - Developmental Biology 2021Quote: ... plasmid encoding Cas9 and gRNA or WT control organoids using QIAamp Fast DNA Tissue Kit (51404, Qiagen). PCR was performed using PrimeSTAR® GXL DNA Polymerase (R050A ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were isolated from 5 mL of overnight liquid culture using QIAprep Spin Miniprep Kit (Qiagen, Germany) and the presence of mutations was confirmed by Sanger Sequencing.
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bioRxiv - Cell Biology 2021Quote: ... CRISPR/Cas9 and donor plasmids were co-transfected into the cell lines using Effectene (Qiagen, Venlo, Netherlands). For drug selection ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA used in transfection of cells was purified using the Qiagen MaxiPrep kit (Qiagen, Valencia, CA).
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bioRxiv - Bioengineering 2022Quote: The plasmid DNAs were linearized with XbaI digestion and purified with Post PCR cleanup kits from Qiagen. The linearized DNAs were used as a template for in vitro transcription with T7 RiboMAX™ Large-Scale RNA Production System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was extracted using the phenol-chloroform protocol combined with Phase Lock Gel tubes (Qiagen GmbH) and was detected by the agarose gel electrophoresis as well as quantified by Qubit® 2.0 (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... After transformation of the cloned vectors and purification of the DNA using EndoFree Plasmid Maxi Kit (Qiagen), the purified products were sent to Microsynth AG (Balgach ...
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bioRxiv - Biochemistry 2021Quote: ... pX330 plasmid DNA expressing the active sgRNA was purified with an endotoxin free kit (Qiagen, Germantown, Maryland). Plasmid DNA concentration was adjusted to 5 ng/ul for pronuclear microinjection (Mashiko et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Random colonies were selected to use in plasmid extraction using QIAprep Miniprep Kit (QIAGEN, Valencia, CA, USA). Positive plasmids were confirmed by PCR reactions ...
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bioRxiv - Developmental Biology 2019Quote: ... The DNA plasmids of ampicillin-resistant bacteria were extracted using the QIAprep Spin Miniprep Kit (Qiagen, 27106) and the sequences confirmed via DNA sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were transfected with the Cignal Reporter Plasmids for GRE using a dual luciferase assay system (Qiagen). Appropriate treatment (siGAS5 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid pENTR-CTDSP1 was extracted from positive colonies using the QIAprep Spin Miniprep Kit (Qiagen) and proper orientation of the cloned fragment was verified by PCR and DNA sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids were purified from the transformed colonies using the QIAprep Spin Miniprep Kit (Qiagen, Valentia, CA, USA) and the mutated regions were confirmed by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... All plasmids used for in vitro experiments were purified using Midiprep kit including endotoxin removal step (Qiagen). pCMV-PE2 was a gift from David Liu (Addgene plasmid # 132775 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid DNA was isolated from bacterial cells using a commercial QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany). Restriction endonucleases were purchased from New England Biolabs (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant plasmids were extracted from cell pellets using the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA, USA). The sequence of the inserts was confirmed by Sanger sequencing (W ...
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bioRxiv - Immunology 2021Quote: ... Phagemid DNA from the third and fourth rounds of phage selection was isolated (Plasmid Miniprep kit, Qiagen) and the genes encoding scFv fragments were ligated into an in-house constructed screening vector providing the secreted scFv with a triple-FLAG tag and a hexahistidine (His6 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°) and plasmids were extracted from the cell pallets using QIAprep Spin Miniprep kit (Qiagen, CA, USA). The sequences of the inserts were confirmed by Sanger sequencing (ACGT Inc ...
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bioRxiv - Bioengineering 2022Quote: ... The phage-containing pellet was then processed using an Endofree Plasmid Giga Kit (Qiagen, cat. code 12391), with the following adjustments ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with 500ng plasmid DNA and a 1:50 Effectene transfection reagent ratio (Qiagen #301425).
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bioRxiv - Molecular Biology 2023Quote: All purification of plasmid DNA or PCR products was done using commercially available kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Plasmids with the desired constructs were generated using an endotoxin free maxiprep kit (12362, QIAGEN, Germantown, MD) and confirmed via whole plasmid NGS ...
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bioRxiv - Cell Biology 2022Quote: ... colonies were scraped off plates and library plasmids purified using a Qiagen HiSpeed Maxi kit (Qiagen, 12662). Plasmids were eluted in Qiagen Buffer TE ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids were recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen) and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was recovered from overnight cultures of positive clones with a QIAquick Spin miniprep kit (Qiagen), and the sequence of the flanking regions was confirmed by in-house sequencing with their respective construction primers and sequencing primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted and purified from 5 ml or 250 ml bacterial culture using Miniprep (Qiagen) or Maxiprep (Thermo-Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid sub-libraries were purified from the bacteria using the QIAamp DNA Mini Preparation Kit (Qiagen). The DNA concentration was measured using the Quant-iT dsDNA (HS ...
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bioRxiv - Bioengineering 2023Quote: ... coli and purified for cell culture application through commercially available kits (Qiagen, Maxi Plasmid Kit, Cat# 12162).
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture with Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Systems Biology 2023Quote: The amplified vectors were extracted from the inoculated culture using Plasmid Plus 96-well Miniprep kit (Qiagen). The concentration of each vector was measured with a Nanophotometer and diluted to 100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... phagemid DNA was extracted from bacterial culture using the QIAprep spin mini prep plasmid extraction kit (Qiagen) with OD260 value was measured by NanoDropTM2000 (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Plasmid DNA was isolated from the pooled transformant cells using a High-Speed Maxi Prep kit (Qiagen).
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bioRxiv - Biochemistry 2023Quote: DNA plasmids were isolated from the overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were harvested by centrifugation and plasmid DNA was isolated using the QIAGEN Midiprep Plus kit (QIAGEN). Plasmid pools ...
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bioRxiv - Developmental Biology 2023Quote: ... The plasmid was linearized by ScaI restriction digest and purified using the QIAquick PCR purification kit (Qiagen). Purified linear plasmid was transfected using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... All colonies were collected by scraping and the DNA was extracted using the Plasmid maxiprep (Qiagen #12165). For a small proof-of-principle library containing 7 different targets ...
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bioRxiv - Cancer Biology 2023Quote: ... Took off the tube and used 30 µL 0.1X EB buffer (From QIAGEN Plasmid Plus Midi Kit) to resuspend the beads and incubated for more than 3 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then performed plasmid extractions from the overnight cell cultures using the QIAprep Spin Miniprep Kit (QIAGEN) for plasmid purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA from single colonies was extracted with the QIAprep Spin Miniprep Kit and Maxi Kits (Qiagen) and confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2023Quote: Protein complexes were produced from the relevant pQE70 plasmid (see Table S2) in strain M15 pREP4 (Qiagen) as follows ...