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Citations for Qiagen :
1501 - 1550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... DESeq2 result were imported into Ingenuity Pathway Analysis (IPA; Service curated by Qiagen; Access provided through the Boston University Genome Science Institute ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Immunology 2024Quote: The extraction of total RNA was carried out utilizing the RNeasy Plus mini kit (Qiagen). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... RNeasy® Plus Universal Mini Kit (Qiagen, Germany) was used to extract the total RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplified products were gel-purified and used for transfecting amoeba cells with the Effectene transfection reagent (QIAGEN). The transfected amoeba cells were subjected to infection with wild-type Mimivirus at an MOI of 10 and incubated till complete cell lysis ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed and RNA was collected using an RNeasy isolation kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and midi-prepped using a Plasmid Plus Midi kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... in a TissueLyser II apparatus (Qiagen Inc., USA; 2 × 3 min, 25 Hz). To remove phenol traces ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from harvested cell pellets using the DNEasy Blood and Tissue kit (Qiagen) according to the manufacturer’s protocol with the addition of a 30 minute RNase digestion at 56°C during the resuspension step ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted from peripheral blood samples from individuals with FECD (QIAgen Gentra Puregene Blood Kit) or from the scleras of the control corneoscleral discs (QIAgen Blood and Tissue kit ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated using RNeasy Plus Mini Kit (QIAGEN). 500 ng of RNA was subjected to reverse transcription using SuperScript IV VILO Master Mix (Thermo ...
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bioRxiv - Developmental Biology 2024Quote: Fetal brain RNA was extracted with RNeasy plus Mini Kits (Qiagen) as previously described81 ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was isolated from somatic embryos using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) with slight modification of the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were vortexed and tissue was disrupted with a a tissue lyser (Qiagen) and □∼□15–20 glass beads (Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... Transposed DNA was purified using a MinElute PCR Purification Kit (Qiagen 28004). Transposed DNA fragments were then amplified using a universal and barcoded primer98 ...
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bioRxiv - Biochemistry 2024Quote: ... Following recovery cells were harvested and genomic DNA was isolated using Maxi kits according to the manufacturer’s protocol (Qiagen). Next-generation sequencing was performed by the Genomics Platform at the Broad Institute.
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was loaded on a Ni-NTA column (Qiagen), and the protein was eluted in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: qPCR was performed in Rotor-Gene Q (QIAGEN) using Maxima SYBR Green qPCR Master Mix (Thermo Scientific™ ...
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bioRxiv - Genomics 2024Quote: ... we extracted genomic DNA (gDNA) using the MagAttract High Molecular Weight kit from Qiagen (Valencia, California). Toe pads extraction of historical study skins was carried out in a dedicated historical DNA extraction laboratory at the Academy of Natural Sciences of Drexel University to reduce contamination risk ...
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bioRxiv - Genomics 2024Quote: ... DNA isolation was then performed using the QiaQuick spin columns and extraction kit from Qiagen (Valencia, CA).
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bioRxiv - Biochemistry 2024Quote: ... and the clarified lysate was applied to a column of Ni-NTA resin (Qiagen and ThermoFisher) preequilibrated in wash buffer 1 (50 mM HEPES ...
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bioRxiv - Genomics 2024Quote: ... DNA extraction was carried out with the sample powder using a Qiagen MagAttract HMW kit (Qiagen Cat. No. 67563) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
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bioRxiv - Immunology 2024Quote: ... and purified with RNeasy Mini Kit (74106, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... Following purification with the PCR Purification Kit (QIAGEN), fragment sizes were confirmed using the 2200 TapeStation and High Sensitivity D1000 ScreenTapes (Agilent) ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Genomics 2024Quote: ... immersed in RNAlater Stabilization Solution (Qiagen Inc., USA) and stored at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... or from the scleras of the control corneoscleral discs (QIAgen Blood and Tissue kit) in accordance with the manufacturer’s guidelines.
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bioRxiv - Cancer Biology 2024Quote: ... quantified by digital PCR (QIAcuity Digital PCR system, Qiagen) at the Biofidelity R&D facility (Cambridge ...
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bioRxiv - Neuroscience 2024Quote: ... The Qiagen RNeasy kit (# NC9307831, Qiagen) was followed per manufacturer’s instructions and Trizol RNA isolation was completed ...
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bioRxiv - Cancer Biology 2024Quote: ... Five 10µm sections from each block were used for total RNA extraction (Qiagen), which was used for RNA integrity determination by RINe score using an Agilent Tapestation and High Sensitivity RNA ScreenTape ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen, 74034) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Bulk tumor-bearing tissues were homogenized in 6 ml of cell lysis buffer (Puregene, 158063, Qiagen) using a FastPrep-24 5G tissue homogenizer (116005500 ...
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bioRxiv - Cancer Biology 2024Quote: ... gel-extracted (QIAquick PCR & Gel Cleanup Kit, Qiagen), and Sanger sequenced (Elim Biopharm ...
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bioRxiv - Cell Biology 2024Quote: ... and STAT2 (GS20847) were from Qiagen (Germantown, MD). Fludarabine was from Selleckchem (Houston ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was added to a 500 μl equilibrated Glutathione Sepharose 4 Fast Flow affinity medium (GE) or Ni-NTA agarose (Qiagen). The lysate was incubated with an affinity matrix for 1 hour at 4 ⁰C ...
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bioRxiv - Molecular Biology 2024Quote: ... and Actin primers were obtained from Quantitect (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: ... IL]) and homogenized using a stainless steel bead in TissueLyser II (Qiagen, Germany) for 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2024Quote: A total of 5 ng of EV-RNA/sample and 100 ng of cellular RNA/sample were used as a template for the preparation of cDNA libraries using the QIAseq miRNA Library Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Molecular Biology 2024Quote: ... Following DNAse treatment with RNase-Free DNase Set (Qiagen), RNA concentration and purity were assessed by Nanodrop 2000 spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 400 ng purified PCR product (PCR purification kit, Qiagen) was denatured by incubating at 95°C for 5 min and the temperature was slowly lowered to < 30°C to allow the formation of heteroduplex ...
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bioRxiv - Neuroscience 2024Quote: ... 20 mg tissue was processed using the Blood and Tissue DNeasy kit (QIAGEN). 100 ng DNA was used for TaqManTM PCR quantification with transgene-specific probe/primer sets normalized to the TERT gene (Supplementary Table 2).
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bioRxiv - Neuroscience 2024Quote: ... RNA was isolated using an RNeasy kit (Qiagen) and reverse transcription was performed with the Quantitect kit (QIAGEN) ...
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bioRxiv - Neuroscience 2024Quote: ... and mRNA was purified using Oligotex beads (QIAGEN). mRNA was treated with EZ-DNase (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse transcription was performed with Quantitect kit (QIAGEN). Spliced transcripts were quantified by TaqManTM PCR using GloX4 primer-probe set specific for transgene exon-exon junction (Table 2) ...
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bioRxiv - Neuroscience 2024Quote: ... and reverse transcription was performed with the Quantitect kit (QIAGEN). Library cDNA was subjected to PCR for Illumina NGS sequencing as described below in the in vivo screening methods.
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from 100–200 mg of tissue using RNeasy plus universal kit (QIAGEN), and reverse transcription was performed with Quantitect kit (QIAGEN) ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from 1-5E5 sorted cells using the DNeasy blood and tissue kit (Qiagen, 69504) and used for barcode amplification and NGS analysis.