Labshake search
Citations for Qiagen :
1451 - 1500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cytosolic fractions were prepared by centrifugation at 10,000 × g for 30 min at 4°C and DNA was isolated from them using the DNeasy Blood & Tissue kit (Qiagen). The copy number of mitochondrial DNA encoding 16S RNA (RNR2 ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA from cell pellets was extracted using the QIAamp Blood Maxi Kit (QIAGEN) and genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (NEB Next UltraII ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was isolated using the commercially available DNeasy Blood and Tissue Kit (Qiagen; Germantown, MD, USA) and RNA was isolated using the commercially available Direct-zol RNA Miniprep Plus Kit (ZYMO ...
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bioRxiv - Molecular Biology 2024Quote: ... pulverized under liquid N2 and DNA was isolated using the DNeasy Blood and Tissue Kit (Qiagen; cat #: 69504) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was purified with Qiagen RNeasy Midi spin columns (Qiagen, Redwood City, CA) with on-column DNase digestion per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: RNA isolation from leaf tissues of five-week-old non-transformed wild type and overexpressing transgenic lines (two replicates of wild type and two transgenic lines) were isolated as per manufacturer’s instruction (Qiagen, Germany). High quality RNA samples with 200 ng/μL concentration were outsourced for RNA-seq ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... The RNA was extracted using RNeasy MinElute kit (Qiagen, Hilden, Germany). In detail ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using QIAamp blood mini-kits (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions and stored at 4°C before use ...
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bioRxiv - Molecular Biology 2024Quote: ... All fragments were then gel-purified using QIAquick Gel Extraction Kit (Qiagen) and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.) ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was performed using the QIAseq FastSelect 5S/16S/23S kit (Qiagen, UK) in accordance with manufacturer’s instructions with the addition of RNase inhibitor (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA was purified with RNeasy mini kit (Qiagen, cat. 50974104). RNA concentration and integrity were checked by fluorometry (Qubit RNA HS ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were removed from the plate with 250µL of RNAprotect Cell Reagent (Qiagen 76104) and stored at -20°C for at least 24 hours prior to extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... were lysed using QIAshredder (Qiagen 79656). Total RNA was then extracted using the RNeasy Mini Kit (Qiagen 74106 ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were resuspended in 400 µL of RLT buffer (Qiagen, cat. 79216s) and RNA was purified with RNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then extracted using the RNeasy Mini Kit (Qiagen 74106) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... a dsDNA template with a T7 promoter incorporated was produced through templateless PCR and purified with MinElute PCR Purification Kit (Qiagen 28004). dsDNA templates were converted to ssRNA through in vitro transcription using MEGAScriptTM T7 Transcription Kit (Invitrogen AM1334 ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated using QIAzol Lysis Reagent and the Qiagen RNeasy kit (Qiagen, Hilden, Germany). Complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in 600 µL of RLT buffer (RNeasy Mini kit, 74104, Qiagen) and stored at -80°C until subsequent RNA isolation ...
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bioRxiv - Microbiology 2024Quote: Cells were grown to 40% confluence and then incubated with 50 nM siRNA and Attractene transfection reagent (QIAGEN) for 36 h according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using the RNeasy Mini kit (74104, Qiagen). Libraries were prepared using the TruSeq® Stranded mRNA Library Prep (20020594 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysed cell material was stored overnight at −80 °C before total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA isolation and RT-qPCR was performed by the University of Rochester Genomics Research Center ...
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bioRxiv - Microbiology 2024Quote: ... and an additional cleaning step running the final volume through an RNA extraction kit column (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... The DNeasy® PowerFood® Microbial Kit (QIAGEN, Germany) was used following the manufacturer’s instructions with minor exceptions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5), 1x rCutsmart Buffer.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from homogenized ileal and colonic tissues by use of the RNeasy Mini kit (Qiagen, Valencia, CA) and reverse transcribed (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Immunology 2024Quote: ... Data analysis was performed using Ingenuity Pathway Analysis (IPA; www.ingenuity.com, Qiagen, CA, USA) with the Core Analysis module to identify significant canonical pathways affected between Th9WT and Th9KO cells ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells at a concentration of 1 μg using Effectene transfection reagent (QIAGEN). Cells were infected with Mimivirus (MOI-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg plasmid DNA (1 μg/μL; prepared by QIAGEN Plasmid Midi Kit) in 30 μL MMG solution was used to transform 200 μL protoplasts by gently mixing with 230μL 40% PEG transformation buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... soluble fraction and Ni-NTA matrix agarose (Qiagen, Germany) were kept for binding for two hours at 4ºC ...
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at 4,000 × g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA minikit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Atrial and vascular tissue was removed and left ventricular myocardium dissected from each heart and homogenized in QIAzol™ reagent (QIAGEN, Maryland, USA). Total RNA containing microRNA was isolated according to manufacturer’s guidelines using miRNeasy spin columns (QIAGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA containing microRNA was isolated according to manufacturer’s guidelines using miRNeasy spin columns (QIAGEN, Maryland, USA). The RNA yield and purity were determined using a NanoDrop ND-1000 (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Following microRNA extraction using miRNeasy columns (QIAGEN, Maryland, USA), the NCode miRNA First-Strand cDNA Synthesis Kit (Thermo-Fisher ...
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bioRxiv - Immunology 2024Quote: ... Two PCRs were performed with the PyroMark PCR kit (Qiagen) in order to report the average methylation of 8 representative CpG sites in the regulatory T cell–specific demethylated region (TSDR ...
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bioRxiv - Immunology 2024Quote: ... with PyroMark Gold Q96 reagents (Qiagen) and Streptavidin Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was purified using the RNeasy kit (Qiagen) or ISOGEN reagent (Nippongene ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA Strep-tactin superflow (Qiagen, Germantown, MD, USA) columns were used ...
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bioRxiv - Immunology 2024Quote: ... and purified using RNeasy Mini Kit (Qiagen) following chloroform extraction ...
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bioRxiv - Immunology 2024Quote: ... PCR products were gel purified (Qiagen) and sequenced on Novaseq 6000 platform (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialysate was loaded onto 5 ml Ni2+-NTA column (Qiagen, Valencia, CA). After washing the column with buffer A containing 20 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: DRIP-qPCR was undertaken using QuantiNova SYBR Green-based PCR (Qiagen) on a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted using a phenol-chloroform method (described below) or with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen). Purified product was delivered to NEB® 10-beta Electrocompetent E ...
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bioRxiv - Molecular Biology 2024Quote: ... on a Rotor-Gene 6000 (Qiagen). Standards were produced by pooling equal amounts of each input and serially diluting 1:10 ...