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Citations for Qiagen :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...
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bioRxiv - Developmental Biology 2024Quote: ... Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen). Transcript abundance was expressed as reads per kilobase of transcript per million mapped reads (RPKM) ...
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bioRxiv - Developmental Biology 2024Quote: ... or a RNeasy Micro kit (74004, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed using the QuantiTect Reverse Transcription Kit (205313, Qiagen). qRT-PCR was performed on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified with the RNeasy MinElute Cleanup Kit (QIAGEN). Approximately 5 to 10 pl of capped mRNAs were microinjected into the cytoplasm of oocytes using a micromanipulator (IM-300 microinjector ...
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bioRxiv - Developmental Biology 2024Quote: ... two independent limbs were microdissected and snap-frozen at −80°C for subsequent total RNA extraction using the RNEasy Mini Kit (QIAGEN, 74134) following protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA extraction was carried out with the RNEasy Micro Kit (QIAGEN, 74004) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and reverse-transcribed using the Quantitect Reverse Transcription Kit (Qiagen # 205311) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Digested chromatin fragments were then purified using the MinElute PCR Purification Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... After being subjected to PCR clean-up (Qiagen), the UTR-library amplicons were then appended with EGFP CDS (derived from EGFP-N1 vector ...
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bioRxiv - Genetics 2024Quote: ... The PCR product was purified again by QIAGEN PCR Purification kit for Illumina® NextSeq paired-end 150 sequencing.
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bioRxiv - Genetics 2024Quote: ... After subjected to PCR clean-up (Qiagen), the UTR-library amplicons were then appended with EGFP CDS and CMV promoter sequence (derived from EGFP-N1 vector ...
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bioRxiv - Genetics 2024Quote: ... separation of the samples was followed by total RNA extraction using the QIAGEN RNeasy Mini Kit (Qiagen catalog number 74104) with on-column DNase I digestion using RQ1 RNase-Free DNase (Promega catalog number M6101).
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bioRxiv - Evolutionary Biology 2024Quote: ... The sampled cellular mass from each clone was transferred into a lysis buffer (DNeasy® Plant Mini Kit, QIAGEN), and cells were disrupted by 1.5-min vortexing (Beadbeater ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA (gDNA) from >1M NPC was extracted using column-based DNeasy Blood & Tissue Kit (Qiagen). DNA integrity was tested on a 0.8% agarose gel and high-quality gDNA was used to prepare libraries according to the NEBNext® Enzymatic Methyl-seq Kit following the section for large insert libraries with minor modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... media was removed and cells were lysed with Qiazol (Qiagen, Cat. #79306). RNA extraction was performed according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... morulae (48 h post-IVF) and blastocysts (68 h post-IVF) were collected in 5uL of 1x TCL lysis buffer (Qiagen #1031586) containing 1% (v/v ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification with Qiagen MinElute PCR Purification Kit (Qiagen, 28004), individual libraries were size selected (100–800 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... Faecal DNA extraction was performed using the PowerSoil® DNA Isolation Kit (Qiagen, Clayton, Victoria, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... which was further verified and purified from gel with a DNA purification kit (Qiagen, Cat.No. 28704). Then ...
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bioRxiv - Developmental Biology 2024Quote: gDNA extractions were done using Qiagen DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA; Cat# 69506). PCR amplification was done using the following primers for targeted region ...
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bioRxiv - Developmental Biology 2024Quote: ... the final plasmids were isolated and purified using the Qiagen Plasmid Maxi kit (QIAGEN, Cat.No.12162). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of samples were processed in parallel to collect RNA with Qiagen RNeasy extraction kits (Qiagen 74034). After isolating total RNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... total RNA was extracted using Trizol (Qiagen) from i5 males (6-8 biological replicates per treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... GFP-positive cells were sorted into REPLI-g cell storage buffer (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Pools of 5 embryos were used for RNA extraction via the Qiagen RNeasy Micro Kit (Qiagen, Valencia CA; Cat# 74004). RNA was eluted in RNase-free water and sent to the New York University Genome Technology Center for RNA-sequencing ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted using the RNeasy plus Mini kit (QIAGEN, Cat.No. A25776). The quality of the RNA was measured using the Agilent 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were processed (Qiagen, 74104) and cytoplasmic and mitochondrial rRNA-deleted stranded Total RNA libraries were prepared using Illumina Truseq RNA Library Prep kit (Illumina 20020589 ...
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bioRxiv - Developmental Biology 2024Quote: ... and isolated total RNA using RNeasy mini kit (Qiagen, Germantown, MD, USA). We generated cDNA from 1 µg of RNA using the SuperScript IV First Stand Synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA was isolated for all study participants in parallel using an RNAeasy Micro Kit (Cat #74004, Qiagen, Valencia, CA). Extracted RNA was quantified with a Qubit fluorometer (Cat #Q33238 ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA purification was performed using the MinElute purification kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: DNA was collected as part of an IRB-approved recruitment protocol and purified using the Qiagen DNeasy Kit and manufacturer’s protocols (Qiagen, USA). Whole genome sequencing was done at Novogene USA to an average depth of at least 30x with standard protocols.
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bioRxiv - Developmental Biology 2024Quote: ... The variant annotation and interpretation analyses were generated through the use of Ingenuity® Variant Analysis™ software https://wwusingtics.com/products/ingenuity-variant-analysis from QIAGEN, Inc ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was extracted from total input and fractionated output of polysome profiling using RNeasy mini kit and cleaned up with DNaseI (QIAGEN). RNA was purified on a 15% polyacrylamide gel and extracted from the gel ...
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bioRxiv - Developmental Biology 2024Quote: ... The plasmid DNAs were linearized by a restriction enzyme and purified with the Qiaquick Gel Extraction Kit (Qiagen) before microinjection ...
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bioRxiv - Developmental Biology 2024Quote: ... http://ghost.zool.kyoto-u.ac.jp/default_ht.html) and quantified using CLC Genomic Workbench version 22.0 (Qiagen).
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bioRxiv - Developmental Biology 2024Quote: The read counts were normalized by calculating the number of reads per kilobase per million (RPKM) for each transcript in each sample using CLC Genomic Workbench version 22.0 (Qiagen). Eight candidate genes showing a minimum 1.5-fold increase following GABA administration ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from homogenized samples using the RNeasy mini kit and treated with DNaseI (QIAGEN, Hilden, Germany). RNA-seq libraries were prepared with NEBNext Ultra I Directional RNA Library Prep with rRNA Depletion (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted with QIAzol and RNeasy Mini kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... RNA was extracted with RNeasy spin columns and digested with DNase I (Qiagen) for 30 minutes at room temperature followed by a column cleanup with RNeasy spin columns ...
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bioRxiv - Genetics 2024Quote: ... and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen, Hilden Germany) in combination ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from the lysate using the DNeasy® Plant Mini Kit (QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and P3c genomic libraries were mapped against the PE-2_H4 genome (GenBank accession number GCA_905220315.1) using the Burrows–Wheeler aligner algorithm implemented by the CLC Genomics Workbench 8.01 (QIAGEN). A cut-off of 0.8 for aligned read length and 0.8 for minimal required identity were set ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted using the MinElute Gel Extraction Kit (Qiagen) according to the manufacturer’s protocol and sequenced by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Developmental Biology 2024Quote: ... These were cloned into Qiagen’s pDrive cloning vector using the Qiagen PCR Cloning Kit (Qiagen) and clones verified by sequencing (Department of Biochemistry Sequencing Facility ...
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bioRxiv - Developmental Biology 2024Quote: RNA was extracted from > 1M NPCs using the RNeasy kit and on-column DNAse digestion (Qiagen). RNA integrity was measured using the Bioanalyzer Nano Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... thresholds were used to prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries using the Ovation RRBS Methyl-Seq System 1-16 (NuGen) and the EpiTect Fast DNA Bisulfite kit (Qiagen). Libraries were sequenced using the NextSeq500 platform (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen). Samples which met quantity (11.75ng/µL ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction was conducted using the RNeasy Mini Kit from Qiagen following the manufacturer’s instructions ...