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Citations for Qiagen :
1301 - 1350 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The resulting plasmid was then transformed into E.coli DH10b strain for isolation via QIAprep Plasmid Mini-Prep Kit (Qiagen). The isolated plasmid was subsequently transformed into D.radiodurans R1 chemically competent cells (above) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using QIAamp blood mini-kits (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions and stored at 4°C before use ...
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bioRxiv - Molecular Biology 2024Quote: ... All fragments were then gel-purified using QIAquick Gel Extraction Kit (Qiagen) and assembled with HindIII-digested pUC19mPheS plasmid using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Inc.) ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was performed using the QIAseq FastSelect 5S/16S/23S kit (Qiagen, UK) in accordance with manufacturer’s instructions with the addition of RNase inhibitor (New England BioLabs ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA was purified with RNeasy mini kit (Qiagen, cat. 50974104). RNA concentration and integrity were checked by fluorometry (Qubit RNA HS ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were removed from the plate with 250µL of RNAprotect Cell Reagent (Qiagen 76104) and stored at -20°C for at least 24 hours prior to extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... were lysed using QIAshredder (Qiagen 79656). Total RNA was then extracted using the RNeasy Mini Kit (Qiagen 74106 ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were resuspended in 400 µL of RLT buffer (Qiagen, cat. 79216s) and RNA was purified with RNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was then extracted using the RNeasy Mini Kit (Qiagen 74106) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... a dsDNA template with a T7 promoter incorporated was produced through templateless PCR and purified with MinElute PCR Purification Kit (Qiagen 28004). dsDNA templates were converted to ssRNA through in vitro transcription using MEGAScriptTM T7 Transcription Kit (Invitrogen AM1334 ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated using QIAzol Lysis Reagent and the Qiagen RNeasy kit (Qiagen, Hilden, Germany). Complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in 600 µL of RLT buffer (RNeasy Mini kit, 74104, Qiagen) and stored at -80°C until subsequent RNA isolation ...
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bioRxiv - Microbiology 2024Quote: Cells were grown to 40% confluence and then incubated with 50 nM siRNA and Attractene transfection reagent (QIAGEN) for 36 h according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated using the RNeasy Mini kit (74104, Qiagen). Libraries were prepared using the TruSeq® Stranded mRNA Library Prep (20020594 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysed cell material was stored overnight at −80 °C before total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). RNA isolation and RT-qPCR was performed by the University of Rochester Genomics Research Center ...
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bioRxiv - Microbiology 2024Quote: ... and an additional cleaning step running the final volume through an RNA extraction kit column (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... The DNeasy® PowerFood® Microbial Kit (QIAGEN, Germany) was used following the manufacturer’s instructions with minor exceptions ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5), 1x rCutsmart Buffer.
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from homogenized ileal and colonic tissues by use of the RNeasy Mini kit (Qiagen, Valencia, CA) and reverse transcribed (High-Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Immunology 2024Quote: ... Data analysis was performed using Ingenuity Pathway Analysis (IPA; www.ingenuity.com, Qiagen, CA, USA) with the Core Analysis module to identify significant canonical pathways affected between Th9WT and Th9KO cells ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells at a concentration of 1 μg using Effectene transfection reagent (QIAGEN). Cells were infected with Mimivirus (MOI-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg plasmid DNA (1 μg/μL; prepared by QIAGEN Plasmid Midi Kit) in 30 μL MMG solution was used to transform 200 μL protoplasts by gently mixing with 230μL 40% PEG transformation buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... soluble fraction and Ni-NTA matrix agarose (Qiagen, Germany) were kept for binding for two hours at 4ºC ...
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at 4,000 × g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA minikit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Atrial and vascular tissue was removed and left ventricular myocardium dissected from each heart and homogenized in QIAzol™ reagent (QIAGEN, Maryland, USA). Total RNA containing microRNA was isolated according to manufacturer’s guidelines using miRNeasy spin columns (QIAGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA containing microRNA was isolated according to manufacturer’s guidelines using miRNeasy spin columns (QIAGEN, Maryland, USA). The RNA yield and purity were determined using a NanoDrop ND-1000 (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Following microRNA extraction using miRNeasy columns (QIAGEN, Maryland, USA), the NCode miRNA First-Strand cDNA Synthesis Kit (Thermo-Fisher ...
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bioRxiv - Immunology 2024Quote: ... Two PCRs were performed with the PyroMark PCR kit (Qiagen) in order to report the average methylation of 8 representative CpG sites in the regulatory T cell–specific demethylated region (TSDR ...
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bioRxiv - Immunology 2024Quote: ... with PyroMark Gold Q96 reagents (Qiagen) and Streptavidin Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was purified using the RNeasy kit (Qiagen) or ISOGEN reagent (Nippongene ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA Strep-tactin superflow (Qiagen, Germantown, MD, USA) columns were used ...
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bioRxiv - Immunology 2024Quote: ... and purified using RNeasy Mini Kit (Qiagen) following chloroform extraction ...
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bioRxiv - Immunology 2024Quote: ... PCR products were gel purified (Qiagen) and sequenced on Novaseq 6000 platform (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: DRIP-qPCR was undertaken using QuantiNova SYBR Green-based PCR (Qiagen) on a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: DNA was extracted using a phenol-chloroform method (described below) or with a DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen). Purified product was delivered to NEB® 10-beta Electrocompetent E ...
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bioRxiv - Molecular Biology 2024Quote: ... on a Rotor-Gene 6000 (Qiagen). Standards were produced by pooling equal amounts of each input and serially diluting 1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... RNeasy® Plus Universal Mini Kit (Qiagen, Germany) was used to extract the total RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplified products were gel-purified and used for transfecting amoeba cells with the Effectene transfection reagent (QIAGEN). The transfected amoeba cells were subjected to infection with wild-type Mimivirus at an MOI of 10 and incubated till complete cell lysis ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed and RNA was collected using an RNeasy isolation kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and midi-prepped using a Plasmid Plus Midi kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... in a TissueLyser II apparatus (Qiagen Inc., USA; 2 × 3 min, 25 Hz). To remove phenol traces ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from harvested cell pellets using the DNEasy Blood and Tissue kit (Qiagen) according to the manufacturer’s protocol with the addition of a 30 minute RNase digestion at 56°C during the resuspension step ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted from peripheral blood samples from individuals with FECD (QIAgen Gentra Puregene Blood Kit) or from the scleras of the control corneoscleral discs (QIAgen Blood and Tissue kit ...
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bioRxiv - Developmental Biology 2024Quote: RNA was isolated using RNeasy Plus Mini Kit (QIAGEN). 500 ng of RNA was subjected to reverse transcription using SuperScript IV VILO Master Mix (Thermo ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA was isolated from somatic embryos using the RNeasy Plant Mini kit (Qiagen, Hilden, Germany) with slight modification of the manufacturer’s protocol ...