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Citations for Qiagen :
1751 - 1800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... cleared lysates were incubated with 2 mL Ni-NTA agarose (Qiagen) on a rotator for 1 hour at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... the RNA samples underwent treatment with the RNase-Free DNase set from QIAGEN. The purified RNA was ultimately resuspended in RNase-Free Water (QIAGEN) ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was purified using the RNeasy Mini Kit (QIAGEN) with the RNA protect Bacteria reagents (QIAGEN) ...
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bioRxiv - Microbiology 2024Quote: ... purified via QIAQuick PCR purification kit (Qiagen), and pET28a vector ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... into 4 million HEK293-GP cells in 300 µl Buffer EC with 16 µl Enhancer and 60 µl Effectene Transfection Reagent (Qiagen 301425) (Morgenstern and Land ...
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bioRxiv - Cell Biology 2024Quote: ... Retro-transcription into cDNA was performed using QuantiTect Reverse Transcription kit (Qiagen).
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bioRxiv - Cell Biology 2024Quote: From pellets of 100 million cells genomic (g)DNA was extracted using the QIAamp DNA Blood Maxiprep Kit (Qiagen) according to manufacturer’s instructions with the following modifications ...
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bioRxiv - Cell Biology 2024Quote: The kit QIAseq FastSelect –5S/16S/23S (Cat# 335921, Qiagen, Hilden, Germany) was used for inhibiting the amplification of ribosomal RNA during library preparation which was then performed from 120ng total RNA of E ...
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bioRxiv - Cell Biology 2024Quote: ... samples were lysed in 5 freeze/thaw cycles (1min in liquid nitrogen; 3min in 37C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). DamID was performed on 200-400 ng genomic DNA (gDNA ...
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bioRxiv - Cell Biology 2024Quote: ... using QuantiTect SYBR Green PCR Kit (QIAGEN, Catalog #204145). Expression of genes in the measurement of arbitrary units was calculated relative to RN18S using the following equation ...
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bioRxiv - Cell Biology 2024Quote: Organoid RNA was isolated using RNAeasy kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The 2X Quantinova SYBR green master mix (Qiagen, 204141). The qPCR reaction was performed in 384 well plates in triplicate reactions ...
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bioRxiv - Cell Biology 2024Quote: ... falciparum 3D7 ring stage parasites were transfected with 100 μg of purified plasmid DNA (Plasmid Maxi Kit, Qiagen, Valencia, CA) by electroporation (310 V ...
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bioRxiv - Cell Biology 2024Quote: ... The amplification product was gel-purified using Qiaquick Gel Extraction Kit (Qiagen, 28706X4), digested with NheI and XhoI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... organs and tissues harvested for RNA or DNA isolation were snap-frozen in liquid nitrogen and homogenized in QIAzol lysis reagent (QIAGEN) or genomic lysis buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was purified using the RNeasy Mini Kit (74106, Qiagen) following the manufacturer’s instructions using steps 4-10 of the “Purification of Total RNA from Animal Cells using Spin Technology” protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were directly lysed in the well using 350 µL of lysing solution (1% 2-mercaptoethanol in Buffer RLT; RNeasy Mini Kit, 74106, Qiagen) by pipetting over the well ...
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bioRxiv - Cell Biology 2024Quote: ... Single colony derived cultures were subsequently mini-prepped using a Qiaprep Spin Miniprep Kit (Qiagen, 27104), and plasmid sequences were confirmed via sequencing by Azenta/GeneWiz ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression changes associated with osteogenesis were assessed using an RT2 Profiler™ PCR Array (PAMM-026Z, Qiagen). A fold regulation threshold of 2 was used to determine up- or down-regulation of each gene in male and female PAE mice compared to control and data is presented as log2 fold change ...
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bioRxiv - Developmental Biology 2024Quote: ... After bead beating with a TissueLyser II (QIAGEN) and 0.5 mm stainless steel beads (Next Advance ...
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bioRxiv - Cell Biology 2024Quote: Erc1 was genetically knocked down in B16-F1 WT cells using specific siRNA oligonucleotides targeting Rab6ip (Erc1) (Qiagen; 1027416). The cells were transfected using Lullaby transfection reagent according to the manufacturer’s instructions with a pool of 10 nM of Mus musculus Rab6ip siRNA (2.5 nM each ...
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bioRxiv - Cell Biology 2024Quote: ... purified over Ni2+-NTA-Agarose (Qiagen) according to standard procedure and eluted in PBHS supplemented with imidazole to 250 mM and pH-adjusted with HCl to 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was precipitated by isopropanol followed by DNaseI digestion (Qiagen).
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bioRxiv - Cell Biology 2024Quote: ... After treatment with wash buffer and the RNeasy Mini Kit (Qiagen), the captured RNAs were extracted for subsequent qRT-PCR analysis.
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bioRxiv - Cell Biology 2024Quote: ... The resulting RNAs were then purified using an RNA Purification Kit (Qiagen, USA).
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bioRxiv - Cell Biology 2024Quote: ... RNA and DNA isolation was performed using the Qiagen All prep kit (Qiagen #80204) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2024Quote: HAEC cells from the stretch experiments were harvested by cell scraping and resuspended in RLT buffer (Qiagen) supplemented with β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... HAEC cultures were stored in RNAprotect (Qiagen #76526). The cells were retrieved by centrifugation at 2,000 rotations per minute (rpm ...
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bioRxiv - Cell Biology 2024Quote: ... followed by purification using QIAGEN-tip20 (QIAGEN, Venlo, Netherlands). To construct the circular double-stranded DNA for pNGS2-BC12-libraries containing either an 8-oxo-G or lesion-free ...
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bioRxiv - Cell Biology 2024Quote: RNA isolation of primary cells or organoids was performed following manufacturer’s instructions using RNeasy Mini kit (Qiagen). Extracted RNA was DNAse treated ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated with the miRNeasy Micro kit (Qiagen) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned-up using the RNeasy kit (Qiagen, UK) and cDNA was synthesized with the High Capacity cDNA Transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The Endo-free plasmid maxi kit (#12162, Qiagen, Valencia, CA, USA) was used for plasmid purification.
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted from zebrafish tissue using a Puregene Core Kit (Qiagen, Germantown, MD). The genomic region containing the desired large deletion was amplified using primers external to each cut site ...
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bioRxiv - Cell Biology 2024Quote: ... The digested plasmid was then purified using the QIAEXII Gel Extraction Kit (Qiagen, Q20021) and diluted to a final concentration of 100 ng/μL and ready for transformation.
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bioRxiv - Cell Biology 2024Quote: ... SK-Mel28 cells were seeded on poly-l-lysine treated glass coverslips at least 36 hours prior to transfection with the in vitro transcribed mRNA using the TransMessenger transfection reagent (Qiagen). One microgram of I-PpoI mRNA and 2μl of Enhancer R were diluted in buffer EC-R to a final volume of 100μl and incubated for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen, Germany)) and qPCR was carried using the StepOnePlus machine (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... NHEKs were transfected with FLG siRNA and the non-targeting control using HiPerFect (Qiagen,UK). For transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and also changing in four other regions) were subject to gene ontology analysis using Ingenuity Pathway Analysis software (Qiagen) was used to perform in depth canonical pathway analysis and determine biological functions altered in the datasets ...
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bioRxiv - Cell Biology 2024Quote: DNA samples 1μg obtained from SMG cells underwent bisulfite conversion using the Epitect Bisulfite Kit (Qiagen). Genomic DNA was denatured by sodium hydroxide treatment ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted with the RNeasy RNA extraction kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: DNA was isolated from individual Drosophila adults using a modified DNeasy Blood and Tissue DNA extraction kit (Qiagen), as previously described (Nelson et al ...
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bioRxiv - Cell Biology 2024Quote: FlexiTube siRNA Premix siRNA (Qiagen, Germany): SI01513407|S5 ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted using the RNEasy Mini Plus kit (Qiagen, Germany). For NHEKs the J2s were removed with a single 5-minute wash with versene prior to lysis ...
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bioRxiv - Cell Biology 2024Quote: ... DNA extraction was then completed using the DNeasy Tissue and Blood Kit (Qiagen, 69504) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Equal quantities of RNA were transcribed into cDNA using QuantiTect Reverse Transcription kits (Qiagen), and analysis of targets gene expression was performed on a 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was from extracted from cultured EnSCs and purified through columns using the RNeasy Plus Universal Mini kit exactly as per manufacturer’s instruction (Qiagen, Manchester, U.K.). Recovered RNA in nuclease-free water was analyzed on a Nanodrop spectrophotometer where concentration and purity (230/280 and 260/280 nm ...
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bioRxiv - Plant Biology 2024Quote: Total DNA was extracted from 0.25 g of soil using the DNA PowerSoil PRO KIT (QIAGEN) according to the manufacturer’s instructions ...