Labshake search
Citations for Qiagen :
1401 - 1450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Live single cells were sorted into 96 wells plates containing 10µl of TCL lysis buffer (Qiagen 1031576), supplemented with 1% β-mercaptoethanol.
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using RNeasy Plus Mini kit (QIAGEN) and subsequently treated with Turbo DNA-free Kit (Ambion) ...
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bioRxiv - Microbiology 2024Quote: ... Cellular RNAs were extracted and purified using a viral RNA extraction kit (Qiagen, Dusseldorf, Germany), and then cDNA was synthesized and monitored with SuperScript-III kit (Takara ...
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bioRxiv - Microbiology 2024Quote: ... Samples were prepared according to the QIAGEN FX DNA Library Preparation Kit (QIAGEN, Germantown, MD, USA). The quality and quantity of the finished libraries were assessed using a Tapestation (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were washed twice in RNAlater and RNA was isolated using the RNeasy Mini kit (Qiagen) following manufacturer’s protocol including DNAse treatment (Turbo DNase kit ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from bacterial colonies grown on TSB with Cm10 and libraries prepared with the QIASeq FX DNA library kit (Qiagen) and sequenced with a MiSeq instrument using MiSeq reagent kit V2 (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR was carried out using the QuantiFast SYBR Green PCR kit (Qiagen) and analyzed on an Applied Biosystems StepOne Real-Time PCR System (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated with the RNAeasy Mini kit (QIAGEN) or Trizol (Thermo 15596-026 ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated using the QIAmp DNA minikit (Qiagen). We utilized a 40-cycle real-time qPCR reaction using 5′-GTTTAGGGAACCGCCATTCTG-3′ forward primer ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... Proteomic and phosphoproteomic data were analyzed using Ingenuity pathway analysis (IPA) (QIAGEN Inc).
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bioRxiv - Microbiology 2024Quote: ... The QIAquick 96 PCR purification kit (Cat. No. 28183, Qiagen, Hilden, Germany) was used to further purify the DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using the reverse transcription kit (Qiagen, Hilden, Germany) and quantitative RT-PCR was performed using gene-specific primers (see Supplemental Table S2 for details ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted using the RNA mini kit (Qiagen, Hilden, Germany). cDNA was synthesized using the reverse transcription kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... or AZD5582 in the presence or absence of KL-2 using an RNeasy kit (Qiagen), with the optional on-column deoxyribonuclease I digestion step ...
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bioRxiv - Microbiology 2024Quote: ... treated with our LRA panel in the presence or absence of KL-2 was carried out with an RNeasy kit (Qiagen), with the optional on-column deoxyribonuclease I digestion step ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified using the QIAquick purification kit (QIAGEN), then cut with XhoI and EcoRI ...
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bioRxiv - Microbiology 2024Quote: Bacterial DNA was extracted from sorted cells and mouse fecal pellets using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as previously described.13 Extracted DNA underwent library preparation and 16S rRNA gene sequencing of the V4-V5 hypervariable region with 515F/926R primers using the Illumina MiSeq PE250 system at the Université de Québec à Montréal (UQAM ...
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bioRxiv - Microbiology 2024Quote: ... and finally gel-purified using the QIAEX II kit (QIAGEN). The purified inserts were ligated in separate reactions into pLPCX(AB ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from samples using Qiagen’s AllPrep Bacterial DNA/RNA/Protein Kit (Cat# 47054; QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from sample filters and purified with a DNEasy Power Water kit (Qiagen). DNA concentrations were quantified using a Qubit (V3 Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were confirmed on 0.8% agarose gel at 90V for 60 min and purified using Qiaquick PCR purification kit according to the manufacturer’s instructions (Qiagen). DNA concentration and quality were assessed ...
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bioRxiv - Microbiology 2024Quote: ... and the homogenates were used for DNA extraction following the insect tissues protocol of the DNeasy Blood and Tissue (Qiagen) kit ...
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bioRxiv - Microbiology 2024Quote: ... corresponding to the three extraction protocols we used: (NM-1) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for mosquito DNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... used for DNA extraction of minipig blood and (NM-3) one negative control for the kit DNeasy PowerWater (Qiagen) used for the DNA extraction of water samples ...
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bioRxiv - Microbiology 2024Quote: ... We followed the standard protocol of the kit DNeasy PowerWater (Qiagen) for the water sample extractions ...
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bioRxiv - Microbiology 2024Quote: ... (NM-2) one negative control for the kit DNeasy Blood and Tissue (Qiagen) used for DNA extraction of minipig blood and (NM-3 ...
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bioRxiv - Microbiology 2024Quote: ... Nuclease-free water (Qiagen, Hilden, Germany) was then applied onto the inner epithelium of the gland ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated using RNeasy Mini Kit (QIAGEN). Three replicates were prepared for each time point and RNA quality was assessed using an Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... DRG and paw skin RNA was extracted using a RLT buffer:2-mercapto ethanol mixture in a 100:1 ratio/RNeasy (Qiagen) RNA mini kit according to the manufacturer’s instructions and spinal cord and spleen RNA was extracted using TRizol (Invitrogen)/RNeasy RNA mini kit ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was purified from the aqueous phase using the Qiagen RNeasy mini kit (Qiagen) following manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The next day tissues were removed from the slide using proteinase K (Qiagen, cat. no. 19131) and cDNA was released from the glass slides and collected in low-binding tubes for cDNA purification and second-strand synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... TAT-SAPIP was purified by Nickel affinity chromatography (Qiagen) following the protocols from the manufacturer.
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation was performed with RNeasy kit (Qiagen). The RNA integrity and concentration was checked using Qubit and 2200 TapeStation (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCl buffer ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from the glia cells using the RNeasy Kit (Qiagen, Milano, Italy) following the manufacturer’s instructions for cells treated with 100 nM or 5μM SAHA in the absence or presence of 10 ng/ml LPS after 4 hours.
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bioRxiv - Molecular Biology 2024Quote: ... Ni-NTA beads (Qiagen) were mixed with lysate supernatant for 30 min and washed with buffer A and eluted with buffer C (25 mM HEPES pH=7.5 ...
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bioRxiv - Neuroscience 2024Quote: RNA from immunopanned astrocytes or neurons from 10-month hCOs was isolated using RNAeasy Micro Kit (Qiagen, 74004). Bulk RNA sequencing libraries were prepared using the NEBNext Ultra II kit with PolyA Selection and sequenced using the Illumina HiSeq 4000 System to a minimum of 30 million paired-end reads per sample ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted for sequencing using RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... Small RNA content was isolated using the miRNeasy Serum/Plasma Kit (Qiagen). Protein content was measured using the Micro BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from MEFs were isolated by RNeasy Plus Mini Kit (Qiagen) as described by the manufacturer after lysing the MEFs by QIAshredder columns (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Labchip analysis was performed to assess the size of small RNAs according manufacturer’s instructions (PerkinElmer).The Reverse transcription was performed on 4 ng RNA using miRCURY® LNA® RT Kit (Qiagen). Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: Total small RNA was extracted from EVs or WS samples using the miRNeasy Serum/Plasma Kit (Qiagen). miRNAs were quantified using Qubit™ microRNA Assay Kits (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: The extracellular vesicles from WS was isolated using miRCURY® Exosome Kit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Molecular Biology 2024Quote: ... frozen MEFs were lysed directly with QIAshredder columns (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: ... as described by the manufacturer after lysing the MEFs by QIAshredder columns (Qiagen). RNA concentrations were measured by nanodrop (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were run on a Rotor-Gene 6000 qPCR machine (Qiagen) and the following thermocycling conditions were used ...
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bioRxiv - Molecular Biology 2024Quote: ... the extracted and DNase treated RNA has been ribodepleted with the QIAseq Fast Select rRNA Plant Kit (Qiagen Hilden, Germany). Double stranded cDNAs were synthesized with random hexamer and the Maxima H Minus Kit (Thermo scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The DNA isolation kit (Qiagen DNeasy, Hilden, Germany) was used to isolate sperm genomic DNA according to the manufacture’s protocol ...