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Citations for Qiagen :
1201 - 1250 of 1933 citations for Mouse DPH7 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Plasmid purification of the overnight cultures was done with QIAGEN Miniprep Kit (QIAGEN, Hilden, Germany) and quantified with the dsDNA HS Qubit Kit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Ampicillin-resistant colonies were grown in liquid culture as a source of plasmid DNA (Qiagen QIAprep Spin Miniprep Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids that had been propagated were then isolated using the QIAgen Miniprep Kit (Qiagen). TAM motifs that were preferentially depleted were identified using amplicon sequencing of the TAM library plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine isolation of the plasmids was performed using the Qiaprep Spin Miniprep kit from Qiagen, USA ...
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bioRxiv - Systems Biology 2023Quote: ... and plasmid DNA was purified from overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen). Proper annealing of sgRNA into the lentiCRISPRv2 backbone was confirmed by Sanger sequencing (Azenta ...
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bioRxiv - Cell Biology 2023Quote: All constructs for bacterial protein expression were cloned in the pQE plasmid (Qiagen, Valencia, CA). WT and mutant TTC1 were cloned downstream of a His14-bdSUMO-GFP tag ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid DNA was purified from the cultures using Qiaprep Spin Miniprep Kit (Qiagen, Germany), digested by appropriate restriction enzymes ...
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bioRxiv - Cancer Biology 2022Quote: ... coli following ampicillin selection using the QIAGEN Plasmid Purification Midi-Prep Kit (QIAGEN, Hilden, Germany) and reconstituted in sterile Tris-EDTA (TE ...
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bioRxiv - Immunology 2023Quote: ... plasmid preparation and DNA gel extraction were performed using nucleotide purification kits purchased from Qiagen or BioBasics ...
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bioRxiv - Molecular Biology 2023Quote: ... and a second 200 ml overnight culture was purified using the Plasmid Maxi Kit (QIAGEN). The new plasmid is named pHW2000-PA-WSN-mScarlet- codon-optimized.
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were screened for the presence of the correct plasmids after miniprep (Qiagen kit) extraction from overnight cultures using restriction enzyme digest and Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: Plasmids were collected from each culture aliquot using QIAprep spin miniprep kits (Qiagen, Hilden, Germany). The pool of barcodes was amplified as previously described (2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was extracted from this culture using the QIAprep Spin Miniprep Kit (Qiagen, 27104). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were purified from overnight cultures using the QIAprep Spin MiniPrep Kit (Qiagen Cat #: 27106). Proper assembly of the construct was screened for by restriction digest of the purified plasmids followed by gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... The clear lysate was transferred to a QIAilter Catridge (From QIAGEN Plasmid Plus Midi Kit) and incubated at room temperature for 10 mins ...
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bioRxiv - Biophysics 2023Quote: ... The in-house plasmid preparation was carried out following the manufacturer’s protocol (Qiagen Midi-kits) and eluted in deionized water ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA was purified following manufacturer’s instructions for the Qiagen Plasmid Maxi Kit (Qiagen, 12162).
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bioRxiv - Genomics 2023Quote: ... to remove the template plasmid and cleaned up using the RNeasy MinElute Kit (Qiagen, 74204). All constructs were polyadenylated using Escherichia coli poly(A ...
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bioRxiv - Immunology 2023Quote: ... Plasmid DNA was purified with the help of the QIAprep Spin Miniprep Kit (#27104, QIAGEN). Successful mutagenesis was verified by Sanger sequencing (LGC Genomics) ...
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bioRxiv - Genetics 2024Quote: ... Minipreparations of plasmids was done using the QIAprep® Spin Miniprep Kit (QIAGEN, Hilden, Germany) and sequenced on both strands using pJET1.2-specific primers (Microsynth AG ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we isolated the plasmids using a Qiagen QIAprep Spin Miniprep kit (Qiagen, Germany, product #27104) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digests were performed after plasmids were isolated using a midiprep kit (QIAGEN, 27104). All riboprobes were generated from linearized plasmids and synthesized using the DIG RNA Labelling Kit (Roche ...
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bioRxiv - Microbiology 2024Quote: ... 1.5x105 Acanthamoeba castellanii cells were transfected with 6 μg of linearized plasmid using Polyfect (QIAGEN) in phosphate saline buffer (PBS) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with AllStars Mouse Negative Control siRNA (Qiagen) or siKras (siKRAS_234 as described by Yuan et al.59 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total mouse RNA was isolated using RNeasy mini kit (Qiagen) and reverse transcribed to cDNA using an oligo (dT ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen mouse liver were first homogenized using TissueLyser II (Qiagen) and then lysed in 1X Laemmli buffer ...
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bioRxiv - Immunology 2022Quote: ... or from frozen mouse BMDMs using RNeasy Mini kit (Qiagen). Reverse transcription (RT ...
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bioRxiv - Developmental Biology 2023Quote: ... RT² Profiler™ PCR Array Mouse Neurogenesis (330231, Qiagen, Germany) was performed according to the manufacturer’s instructions on LightCycler 96 (Roche ...
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bioRxiv - Physiology 2023Quote: ... RT2 Profiler PCR Array for Mouse Fibrosis (PAMM-120Z, Qiagen) and Qiagen GeneGlobe Analysis was used to evaluate fibrosis between normal saline and experimental groups at each time point.
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... plasmids encoding putative interactors were transfected in triplicate using the Effectene transfection reagent (Qiagen, Hilden, Germany) and a final concentration of 400µM BSA-bound oleic acid were added ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was then extracted from chosen colonies using a Qiaprep spin Mini-prep kit (Qiagen) and sequenced (Applied Biosystems’ 3730 DNA Analyser ...
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bioRxiv - Microbiology 2021Quote: ... The plasmid containing the cloned chitinase gene was isolated using the Qiaprep spin miniprep kit (Qiagen). Restriction digestion of the plasmid was done with XhoI and XmaI ...
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bioRxiv - Genomics 2020Quote: ... The plasmid DNAs were prepared using QIAprep Spin Miniprep Kit (catalog No. 27104, QIAGEN, Beijing, China). The appropriate restriction enzymes were then used to determine the presence or absence of methylated motifs in recombinant plasmid ...
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bioRxiv - Plant Biology 2019Quote: ... pDONR207-sgGBSS1 and pDONR207-sgGBSS2 were purified using the QIAGEN Plasmid Plus Midi Kit (QIAGEN, Germany), followed by a sodium acetate precipitation.
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bioRxiv - Bioengineering 2020Quote: Genomic and plasmid DNA were extracted from transformants using a DNeasy Blood & Tissue kit (Qiagen, Germany) and Exprep plasmid SV kit ...
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bioRxiv - Cancer Biology 2020Quote: Plasmid was extracted from 1.5 ml of bacterial culture with the QIAamp DNA Mini Kit(Qiagen) and purified for sequencing validation (1st BASE) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were co-transfected with the C6orf203-TAP construct and with plasmid pOG44 using Superfect (Qiagen). After 48h ...
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bioRxiv - Bioengineering 2021Quote: ... and only the plasmid DNA was separated through the column (Qiagen, QIAquick® PCR purification Kit).
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bioRxiv - Immunology 2021Quote: ... Plasmid DNA was prepared from overnight cultures using the QIAprep Spin Miniprep kit (QIAGEN, Hilden, Germany) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Isolation and purification of the pDNA was done using a Plasmid Plus maxi kit (QIAGEN, UK) and a NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Purified PCR products amplified from plasmids isolated from a single bacterial colony (QIAprep Spin MiniprepKIT, Qiagen) were labeled by random priming with digoxigenin-11-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Agarose gel purification and DNA plasmid extractions were performed using a QIAquick Gel extraction kit (QIAGEN). Restriction enzyme digests were performed according to manufacturer recommendations (Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid or oligo-derived transcription templates were cleaned up using a MinElute PCR Purification Kit (Qiagen). SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... Mary Goldring) and 10 ng of pRL Renilla luciferase plasmid using the Effectene transfection reagent (Qiagen). Cells were transfected for 8 hrs and then cultured for 24 hrs ...
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bioRxiv - Genomics 2022Quote: ... and plasmid DNA of positive clones was obtained using QIAprep Spin Miniprep Kit (Qiagen, Cat#: 27106). Clones were verified as mutation-free by capillary sequencing ...