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Citations for System Biosciences :
51 - 100 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and the lentiviral particles were produced using the pPACKH1 HIV Lentivector Packaging System (System Bioscience Cat No. LA500A-1). In brief ...
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bioRxiv - Molecular Biology 2024Quote: ... the filtered supernatant underwent a 100-fold concentration step using PEG-it Virus Precipitation Solution (System Biosciences), according to the manufacturer’s protocols.
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bioRxiv - Immunology 2024Quote: ... and exosome size was analyzed with a nanoparticle tracking analyzer (Nanosight NS300) and expression of exosomal marker proteins verified via Exo-Check™ Antibody Array (System Biosciences, Palo Alto, CA).
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bioRxiv - Cancer Biology 2024Quote: These are antibody-capture arrays for semi-quantitative detection of 8 putative markers of exosomes/EVs (EXORAY200B, System Biosciences/SBI, Palo Alto, CA, USA). These were performed according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Full length FcγRIIIa variant sequences were cloned under the CMV promoter in pCDH-CMV-MCS- EF1α-copGFP (System Biosciences). These three plasmids were cotransfected into HEK293T cells using Fugene 6 (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Exosome isolation from mouse serum was performed using an ExoQuick precipitation reagent (System Biosciences, EXOQ5A-1) and a Fixed-Angle Rotor TLA120.1 (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: AFSC-EV RNA cargo was labeled via Exo-GLOW™ Exosome Labeling kit (SBI System Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Exosomes were isolated from the serum sample using ExoQuick™ Exosome Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were transiently transfected with a retroviral MSCV-BCR/ABLp185 vector (kindly provided by Veronika Sexl) mixed with PureFection transfection reagent (System Biosciences). The bone marrow cells were transduced for 1 hour with the fresh viral supernatant in the presence of 10 µg/ml polybrene (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: The clonal control iPSC lines SC102A-1 (System Biosciences) and CRTDi011-A (this study) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The sfGFP expression was induced on day 3 post-injection by intraperitoneal (I.P.) injection of 3.0 mg/mouse of water-soluble cumate (System Biosciences, #QM150A-1). Mice were imaged with IVIS or sacrificed at indicated times for ex vivo imaging ...
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bioRxiv - Cell Biology 2024Quote: Conditioned media (n=2 each group) were sent for Exo-NGS™ services (System Biosciences, CA, USA). In brief ...
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bioRxiv - Cancer Biology 2024Quote: ... The N-term Flag-HA-MED4 cDNA was sub-cloned in pCDH-EF1-MCS-IRES- Neo (System Biosciences; CD502A-1) and verified by sequencing ...
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bioRxiv - Immunology 2024Quote: ... U251-GFP and U87-RFP cells were generated via lentiviral transduction of parental cell lines with the lentiviral vectors pBLIV-MSCV-GFP-T2A-Fluc and pBLIV-CMV-RFP-T2A-Fluc (System Biosciences Inc, Palo Alto, USA), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA constructs were transferred to the FC550A-1 backbone (System Biosciences) belonging to their ϕC31 integrase vector system using the same restriction sites ...
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bioRxiv - Bioengineering 2024Quote: HEK293T cells stably expressing mRNA constructs were generated using the ϕC31 integrase system (System Biosciences) according to the manufacturers protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... mixed with cold PEG-it Virus Precipitation Solution (System Biosciences) at 4:1 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... shE3C (5’-CCATATACCGCAAGGAATA-3’) and shNOXA (5’-GGTGCACGTTTCATCAATTTG-3’) were cloned into pGreenPuro plasmid (System Biosciences) with BamHI and EcoRI restriction sites and the loop GTGAAGCAGATG was in between the sense and antisense strand ...
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bioRxiv - Developmental Biology 2024Quote: ... miRZip anti-miR precursor constructs for miRZip-129-5p and pGreenPuro Scramble Hairpin control constructs were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: CRC cells were infected using Human Nanog pGreenZeo Differentiation Reporter (System Biosciences SR10030VA-1) (www.systembio.com ...
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bioRxiv - Neuroscience 2024Quote: ... and PEG-It Virus Precipitation Solution (cat # LV825A-1) was from System Biosciences. Macrophage colony stimulating factor (M-CSF ...
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bioRxiv - Genetics 2024Quote: ... Super PiggyBac Transposase (PB210PA-1) was purchased from System Biosciences.
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bioRxiv - Synthetic Biology 2024Quote: ... The Cas9-T2A-TdT transgene(s) with the TdT variant(s) of interest were either stably integrated into the genome with Super PiggyBAC Transposase (System Biosciences Innovation, PB210PA-1) (for long-term propagation of the CHYRON locus as in Figure 4b-c ...
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bioRxiv - Molecular Biology 2024Quote: ... together with the HR 110PA-1 donor vector (System Biosciences) in which two homology arm sequences were cloned ...
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bioRxiv - Microbiology 2024Quote: ... Lentivirus particles were concentrated with PEG-it (LV825A-1, System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... rKSHV.219 particles then were concentrated with PEG-it (LV825A-1, System Biosciences) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: cDNA for human TMEM30a was obtained from TransOMIC and subsequently cloned into pCDH-MSCV-T2A-Puro vector (System Biosciences) with and without a C-terminal HA-tag ...
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bioRxiv - Bioengineering 2024Quote: ... The human KRT16 pseudogene 5 (K16P5) enhancer and promoter sequence was amplified from pRZ-hKeratin (Cat# SR900CS-1, System Biosciences). The SCP3 core promoter [15] was synthesized by Genewiz ...
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bioRxiv - Molecular Biology 2024Quote: ... The plasmid was constructed by using pCDH_MSCV (System Bioscience) vector backbone carrying spCas9 cDNA from Addgene #48138 and mCat-1 cDNA from Addgene #17224 (see Note 1).
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bioRxiv - Molecular Biology 2024Quote: ... (EEV604A-2; System Bioscience, Palo Alto, CA, USA), mCherry plasmid (given by Dr ...
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bioRxiv - Neuroscience 2024Quote: ... lentivirus expressing pLV-mU6-scrambled-shRNA::SYN-tdTomato or pLV-mU6-circHomer1a- shRNA::SYN-tdTomato (1.2×109 IFU/mL, System Biosciences) (gift from N ...
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bioRxiv - Neuroscience 2024Quote: Short hairpin RNA (shRNA) sequences were manually designed and cloned into the restriction sites EcoRI & BamHI in the pGreenPuro vector (System Biosciences, SI505A-1). shRNA duplexes were annealed and inserted using traditional ligation cloning ...
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bioRxiv - Immunology 2024Quote: ... The remaining supernatant was mixed with 63 µL ExoQuick (System Biosciences) reagent and incubated at 4 ℃ overnight ...
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The G3BP Stress-Granule Proteins Reinforce the Translation Program of the Integrated Stress ResponsebioRxiv - Molecular Biology 2024Quote: ... along with the super PiggyBac transposase expression vector (System Biosciences #PB210PA-1), was reverse transfected into the parental cell line using lipofectamine 3000 transfection reagent (Thermo #L3000015 ...
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bioRxiv - Immunology 2024Quote: ... into PB-CMV-MCS-EF1a-Puro PiggyBac vector (PB501b-1, System Biosciences) to generate PB-CMV-cOVA-EF1a-Puro plasmid which was delivered together with piggybac transposon (a gift from A ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids and lentivector pPACKH1 packaging plasmids (System Biosciences) were then transfected into 293T cells at 70-80% confluence by GeneJuice (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: 16 million K562 cells (8 million K562-VP64 cells and 8 million K562-VPR cells) were transfected with the gRNA library and the piggyBac transposase (System Biosciences, Cat. No. PB210PA-1) at a 20:1 molar ratio of library:transposase using a Lonza 4D nucleofector and the Lonza nucleofector protocol for K562 cells ...
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bioRxiv - Bioengineering 2024Quote: ... were purchased from the Riken Brain Science Institute (Japan) and used to generate lentivirus particles by co-transfecting HEK 293TN cells (System Biosciences) with packaging (psPAX2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Western Blotting of EVs protein was performed using slot blot EXO-Array Exosome antibody check kit (System Biosciences, CA). Antibody slots for CD63 ...
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bioRxiv - Cancer Biology 2024Quote: ... EV RNA was isolated by EXOQUICK® Exosome isolation and RNA purification kit for serum/plasma (System Biosciences,CA) and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293T cells on a 60 mm dish were co-transfected with 8 µg of PB-puro-CMV-POST-IT-Tet-SBPK4R-sPupK61R-iresGFP and 2 µg of the Super PiggyBac Transposase expression vector (PB200PA-1, System Biosciences, USA). Four days later ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were transfected with the corresponding PiggyBac vector and the Super PiggyBac Transposase Expression Vector (System Biosciences) and were then subjected to selection with G418 (400 μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... The stable cell lines of U2OS cells with the Tet-on inducible system were constructed with the PiggyBac Transposon system (System Biosciences) containing a puromycin selection gene ...
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bioRxiv - Molecular Biology 2024Quote: ... then cloned into a PB-EF1α-MCS- IRES-GFP PiggyBac expression vector (System Biosciences, cat # PB530A-2). HaloTag (Los et al ...
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bioRxiv - Molecular Biology 2024Quote: ... Super PiggyBac Transposase Expression Vector (System Biosciences, cat # PB210PA-1) was transfected with PB-EF1α-RPL22-HaloTag-P2A-puro and PB-EF1α-RPL22L1- HaloTag-P2A-puro to efficiently integrate the inserts into the genome ...
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bioRxiv - Molecular Biology 2024Quote: ... and HaloTag only expressing HCT116 p53+/+ cells were generated using PiggyBac transposon system (System Biosciences) and selected with puromycin (1 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: The EGFP-PIF1 expression construct was generated by placing the EGFP tag on the N-terminus of human PIF1 cDNA and subcloned into the lentiviral vector pCDH-CMV-MCS-EF1-PURO (System Biosciences) at the NotI and NheI cloning sites ...
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bioRxiv - Neuroscience 2024Quote: ... supernatant were mixed with 252 μl of ExoQuick precipitation solution (EXOQ; System Biosciences, Inc., Mountain View, CA), and incubated for 1 hour at 4°C ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... 5 µL of thrombin (System Bioscience) was gently mixed with 500 µL plasma aliquot each and incubated at RT for 30 min ...
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Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neuronsbioRxiv - Neuroscience 2024Quote: ... 5 mL supernatant processed as above was gently mixed with 1 mL of ExoQuick (System Bioscience) each and incubated quiescent at 4°C overnight and then centrifuged at 1,500 x g for 20 min at 4°C to retrieve EVs in the pellet ...