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Citations for System Biosciences :
1 - 50 of 677 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Viral transduction of matured iPS-RPE were aided using TransDux virus transduction reagent (System Biosciences, cat# LV850A-1) and spin infection (800 g ...
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bioRxiv - Microbiology 2024Quote: ... EVs were enriched from RBC culture supernatants using ExoMaxTM according to manufacturer’s instructions (System Biosciences, Palo Alto, CA). EVs were examined for protein concentration using BCA and by Western blots as described below.
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bioRxiv - Cancer Biology 2024Quote: ... Medium containing lentiviral particles was collected at 48 and 72 hours post transfection and concentrated using PEG-it Virus Precipitation Solution (System BioSciences). Precipitated lentiviral particles were resuspended in DMEM ...
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bioRxiv - Bioengineering 2024Quote: ... and the EVs were isolated with a commercially available precipitation kit (ExoQuick, System Biosciences, Palo Alto, CA, US) following the protocol provided by the vendor ...
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bioRxiv - Cell Biology 2024Quote: ... Extracellular vesicles were isolated from 10 mL of cell culture supernatant using the ExoQuick-TC® Exosome Precipitation Solution (cat# EXOTC10A-1; System Biosciences) according to the manufacturer’s instructions with a few modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatant was combined with PEGit concentrator (5X; System Biosciences) overnight at 4°C and centrifuged at 1500 g for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Cancer Biology 2024Quote: ... 20% TransDux MAX Lentivirus Transduction Enhancer (LV860A-1, System Biosciences), and 0.5% TransDux (LV860A-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... A water-soluble cumate solution (QM150A-1, System Biosciences) was used to induce PAX3::FOXO1-T2A-mCherry ...
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bioRxiv - Molecular Biology 2024Quote: ... we utilized the MC-Easy™ Minicircle DNA Production Kit (System Bioscience, SBI). Briefly ...
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bioRxiv - Neuroscience 2024Quote: pCDH-EF1-MCS-IRES-copGFP lentiviral plasmid (System Biosciences) was used as the backbone to create (G4C2)92 and (G4C2)2 lentiviral constructs ...
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bioRxiv - Neuroscience 2024Quote: Total RNA isolation kits were employed to isolate RNA from cultured iMicroglia (n=4/group; System Bioscience, Palo Alto, CA, USA) and the mouse hippocampal tissues (n=5-6/group ...
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bioRxiv - Immunology 2024Quote: ... and Transposase Expression vector DNA (System Biosciences). Cells were cultured with 2 µg/mL puromycin (Life technologies) ...
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bioRxiv - Neuroscience 2024Quote: LDLR and spaghetti monster plasmids were designed by System Biosciences. The AAV-BR1 packaging vector was a gift from Jacob Korbelin ...
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bioRxiv - Neuroscience 2024Quote: ... and the medium was changed to the same DMEM but supplemented with 10% exosome-depleted FBS (EXO-FBS-50A-1, System Bioscience). The media was collected on the day of isolation ...
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bioRxiv - Developmental Biology 2024Quote: ... with the primers 5’-GGGGAATTCGCCACCATGGGTTCTCA-3’ and 5’-CCC GCGGCCGCTCACTTCGCTGTCATCA-3’ was subcloned into EcoRI and NotI sites in the P B-CMV-MCS-EF1α-Puro PiggyBac transposon vector (PB510B-1, System Bioscience).
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bioRxiv - Developmental Biology 2024Quote: ... The expression vector coding GCaMP6s was introduced into MDCK cells with Super PiggyBac transposase (System Bioscience). After a few days ...
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bioRxiv - Cell Biology 2024Quote: ... The EPAC1-V5-APEX2 fragment was linearized with EcoRI and NotI and cloned into the pCDH-CMV-MCS-EF1α-Puro (System Biosciences, Catalog no. CD510B-1) lentiviral vector ...
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bioRxiv - Cell Biology 2024Quote: ... The sequence was inserted into lentiviral expression vector pCDH533-IRES-Neo (System Biosciences) to become pCDH533-HA-hK6a-Flag-IRES-Neo ...
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bioRxiv - Immunology 2024Quote: ... and pCDH-EF1α-T2A-copGFP (System Biosciences) for expression in HEK293T or tagged with FLAG-HA then cloned into the pUC57-mini vector (synthesized by Genscript ...
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bioRxiv - Immunology 2024Quote: ... The HEK293TN-hACE2 cell line was generated by lentiviral transduction of HEK293TN (System Bioscience, Cat#LV900A-1) cells as described in Notarbartolo S ...
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bioRxiv - Genomics 2024Quote: ... and NheI and NotI restriction sites were used to clone the insert into the pCDH-EF1-MCS-T2A-GFP lentiviral vector (System Biosciences, Palo Alto, CA). OE21 tumor cells were then transduced with the resultant lentivirus ...
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bioRxiv - Cell Biology 2024Quote: Stable cell lines expressing GFP-tagged cytoplasmic tail chimeras under a cumate-inducible promoter were generated by PiggyBac transposition (System Biosciences). Wild-type ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were immediately cryopreserved and maintained in selection medium containing 60 μg/mL cumate (System Biosciences) for at least one passage prior to assay.
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Cell Biology 2024Quote: ... pseudoviral particles were produced using 293TN cells (System Biosciences, SBI). The 293TN cells were transfected with the lentiviral plasmid of interest as well as lentiviral packaging and envelope plasmids (psPAX and pMD2.G ...
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bioRxiv - Cell Biology 2024Quote: ... PAAKRVKLD) from pCDH-NLSSV40-sfCherry-NLSMyc-Blast (30) were ligated to pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1, System Biosciences) using the Ligation-Convenience Kit (Nippon Gene ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-CD63 (rabbit, System Biosciences, Palo Alto ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The mouse H3f3a sequence (encoding the histone H3.3A isoform) upstream an IRES and nuclear GFP sequences were cloned into the EcoRI and NotI sites of EEV600A-1 (System Biosciences) to generate the EEV expressing the WT histone H3.3A (supplementary Fig ...
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bioRxiv - Immunology 2024Quote: ... and exosome size was analyzed with a nanoparticle tracking analyzer (Nanosight NS300) and expression of exosomal marker proteins verified via Exo-Check™ Antibody Array (System Biosciences, Palo Alto, CA).
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bioRxiv - Immunology 2024Quote: ... Full length FcγRIIIa variant sequences were cloned under the CMV promoter in pCDH-CMV-MCS- EF1α-copGFP (System Biosciences). These three plasmids were cotransfected into HEK293T cells using Fugene 6 (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Exosome isolation from mouse serum was performed using an ExoQuick precipitation reagent (System Biosciences, EXOQ5A-1) and a Fixed-Angle Rotor TLA120.1 (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: These are antibody-capture arrays for semi-quantitative detection of 8 putative markers of exosomes/EVs (EXORAY200B, System Biosciences/SBI, Palo Alto, CA, USA). These were performed according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Exosomes were isolated from the serum sample using ExoQuick™ Exosome Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... miRZip anti-miR precursor constructs for miRZip-129-5p and pGreenPuro Scramble Hairpin control constructs were purchased from System Biosciences (Palo Alto, CA), and lentiviruses were generated according to the manufacturer’s protocol.
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bioRxiv - Genetics 2024Quote: ... Super PiggyBac Transposase (PB210PA-1) was purchased from System Biosciences.
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bioRxiv - Synthetic Biology 2024Quote: ... The sfGFP expression was induced on day 3 post-injection by intraperitoneal (I.P.) injection of 3.0 mg/mouse of water-soluble cumate (System Biosciences, #QM150A-1). Mice were imaged with IVIS or sacrificed at indicated times for ex vivo imaging ...
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bioRxiv - Cancer Biology 2024Quote: ExoQuick-TC® ULTRA for the isolation of exosomes from Tissue Culture Media (EQULTRA-20TC-1; System Biosciences). Antibodies used were all mouse primary monoclonal antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... the Exo-FectTM Exosome Transfection Kit (System Biosciences, USA) was used following manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... U251-GFP and U87-RFP cells were generated via lentiviral transduction of parental cell lines with the lentiviral vectors pBLIV-MSCV-GFP-T2A-Fluc and pBLIV-CMV-RFP-T2A-Fluc (System Biosciences Inc, Palo Alto, USA), respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... mixed with cold PEG-it Virus Precipitation Solution (System Biosciences) at 4:1 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... shE3C (5’-CCATATACCGCAAGGAATA-3’) and shNOXA (5’-GGTGCACGTTTCATCAATTTG-3’) were cloned into pGreenPuro plasmid (System Biosciences) with BamHI and EcoRI restriction sites and the loop GTGAAGCAGATG was in between the sense and antisense strand ...
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bioRxiv - Cancer Biology 2024Quote: ... Exosome enriched EVs (hereafter EVs) were isolated from 500 µl serum using EXOQUICK (System Biosciences, CA) exosome precipitation method by standard protocols provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transduced with lentiviral CD513B vector (System Biosciences) or CD513B-ERCC1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 g of omentum tissue was minced with sterile razor blades and cultured in 10 mL DMEM/F12 media supplemented with 1% exosome-depleted fetal bovine serum (System Biosciences, Palo Alto, CA). ACM was collected the following day ...
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bioRxiv - Genomics 2024Quote: 16 million K562 cells (8 million K562-VP64 cells and 8 million K562-VPR cells) were transfected with the gRNA library and the piggyBac transposase (System Biosciences, Cat. No. PB210PA-1) at a 20:1 molar ratio of library:transposase using a Lonza 4D nucleofector and the Lonza nucleofector protocol for K562 cells ...
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bioRxiv - Cell Biology 2024Quote: ... the piggyBac expression vector and Super PiggyBac transposase expression vector (PB210PA-1, System Bioscience) were introduced into the cells using a 2-step pulse electroporator (NEPA21 ...