Labshake search
Citations for Mirus Bio :
601 - 650 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 1μg each of the eight plasmids were mixed well with 16 μL of TransIT-LT1 (Mirus Bio) and transfected into the hMDCK-HEK293 cocultured cells at 70% confluence ...
-
bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... 293T cells were transfected with a plasmid expressing SARS-CoV-2 Spike (kindly provided by Markus Hoffmann and Stefan Pöhlmann Hoffmann et al., 2020 PMID: 32142651) using TransIT-X2 (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: Plasmid transfections to generate stable cell lines were performed with TransIT®-LT1 transfection reagent (Mirus). 2×105 cells per well were seeded in 6-well plates the day before transfection ...
-
bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.5 µg of plasmid DNA per well using TransIT-LT1 transfection reagent (Mirus Bio) in OptiMEM (Gibco) ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfection mixtures were prepared with 24 μl LT1 (Mirus-Bio), 800 μl Opti-MEM (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... using TransIT-LT1 transfection reagent (Mirus Bio). The pseudoviruses were then harvested and concentrated at 48 hours after transfection ...
-
bioRxiv - Microbiology 2023Quote: ... Huh7 cells in 6- well plates were transfected with 3 μg of each in vitro transcribed RNA using the TransIT mRNA transfection kit (Mirus Bio) as previously described (33) ...
-
bioRxiv - Microbiology 2023Quote: ... along with 4.5 uL of TransIT-LT1 reagent (Mirus Bio LLC; MIR2305). VSVG pseudotyped lentivirus was harvested and filtered through a 0.22 um filter (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... or TransIT®-293 Transfection Reagent (MIR2700, Mirus Bio LLC, Madison, WI, USA). For production of VSV-G-pseudotyped HIV-1 ...
-
bioRxiv - Immunology 2023Quote: ... All plasmid DNA transfections were conducted using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... 60 µl transfection reagent TransIT-293 (Mirus Bio) was added and incubated for 30 min at RT and added to the 10 cm dish with HEK293T cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... were packaged in HEK 293T cells as previously described [15] using Turbofect (Fisher, UK) or trans-IT (Mirus. USA) as lipofection reagents ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected to produce lentiviral particles with TransIT-Lenti transfection reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... For CPV infections, MEF cells was transfected with feline TfR1 plasmid (pCTfR, 2.5 μg)25 by using TransIT-LT1transfection reagent (Mirus Bio, WI, USA). To generate MEFDyn1,2,3 TKO cells stably expressing hACE2 ...
-
bioRxiv - Microbiology 2023Quote: ... via TransIT-X2 (Mirus). After 24 hr post-transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... TransIT (Mirus) was used to transfect the plasmids ...
-
bioRxiv - Molecular Biology 2023Quote: ... On day 2 cells were transfected with lentiviral DNA following the Mirus-LT1 (Mirus MIR 2300) transfection protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 ng pEZY_AR plasmid and either 100 ng pEZY_eGFP or pEZY_Flag_HOXB13 using TransIT-2020 (Mirus) and then plated in DMEM (Gibco ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected with pCAG-Cas9-EGFP (gift from Randy Platt) and sgRNAs plasmids with TransIT-LT1 Transfection Reagent (Mirus, MIR2305) and after 48 h were sorted with BD FACSAria III Cell Sorter (BD FACSDiva Software ...
-
bioRxiv - Genomics 2023Quote: ... using Transit-IT-293 transfection reagent (Mirus) (Stewart et al. ...
-
bioRxiv - Genetics 2023Quote: ... using the TransIT Lentivirus transfection reagent (MIR 6600, Mirus Bio, Madison, WI, USA). gRNA libraries were designed from Brunello gRNAs in seven sub-pools with 4-gRNAs per gene and 5% Non-Target (Table S1) ...
-
bioRxiv - Immunology 2023Quote: ... Lentivirus were produced with HEK293T cells and Mirus TransIT Lentivirus System (Mirus, 6650) according to the manufacturer protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... hiPSCs are transfected with ETV2 mmRNA in mTeSR™-E8™ complete media using TransIT reagent and mRNA boost ((Mirus Bio) One hour prior to transfection ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-Lenti Trasfection Reagent (6605, Mirus). CH157-MN cells were stably transduced with lentiviral particles to generate CH-157MN NOTCH3ICD or empty pLVX vector (EV ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced by transient transfection of Phoenix-ECO cells (CRL-3214) using TransIT®-LT1 Reagent (Mirus Bio LLC, Madison, WI, USA). The lentiCRISPR construct or the pLJM1-EGFP plasmid (Addgene plasmid #19319 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and TransIT-X2 (Mirus, #MIR 6000).
-
bioRxiv - Biochemistry 2023Quote: ... 48 uL TransIT-293 transfection reagent (Mirus Bio LLC, catalog no. MIR 2704) diluted with 1300 uL serum-free media (SFM ...
-
bioRxiv - Cancer Biology 2023Quote: ... TransIT-X2 Dynamic Delivery system (Mirus) was used for transfection following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: HEK293T cells were transiently transfected using TransIT-LT1 (Mirus #MIR2306) according to the manufacturer’s protocol at a ratio of 3µL of transfection reagent per 1µg of DNA and the medium was replaced 24 hours post transfection ...
-
bioRxiv - Neuroscience 2023Quote: ... These SGCs were transfected with WT or mutant GPR37L1 using TransIT®-LT1 reagent (Mirus Madison, WI, USA, Cat Bo.MR2300). The control and mutant GPR37L1 or KCNJ3 expression levels were examined by Real-time PCR.
-
bioRxiv - Cancer Biology 2023Quote: ... TransIT-LT1 (Mirus Bio) was added dropwise ...
-
bioRxiv - Molecular Biology 2023Quote: ... the generated vectors and the PIK packaging plasmid were delivered into HEK 293T cells using the TransIT-LT1 transfection reagent (Mirus Bio). Stable MEF cell lines expressing either wild-type (WT ...
-
bioRxiv - Microbiology 2023Quote: ... 1 μg pWPI-Luc2 (a reporter plasmid encoding a firefly luciferase gene14) and 500 ng plasmids expressing parental S protein or its derivatives using TransIT-293 transfection reagent (Mirus, Cat# MIR2704) or TransIT-LT1 (Takara ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the lentivector were transfected into 10 million 293T using TransIT-LT1 reagent (Mirus). Supernatants were harvested at 48 and 72 hrs after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: HeLa cells inducibly expressing GFP and Flag-HA-tagged UNKWT were seeded in 6-well dishes and transfected 24 hours later at about 40% confluence using the TransIT-X2 Dynamic Delivery System (Mirus, MIR6003) with a pool of siRNAs targeting CNOT7 (Horizon ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 7.5ul TransIT-LT1 (Mirus) transfection reagent ...
-
bioRxiv - Physiology 2023Quote: We cloned the coding sequences of human TSHB and CGA into pLIVE vectors (Mirus Bio, Madison, WI) using a polymerase chain reaction (PCR ...
-
bioRxiv - Cell Biology 2023Quote: ... Ingenio® electroporation buffer (Mirus, Ref. MIR 50111), HiPerFect transfection reagent (Qiagen) ...
-
bioRxiv - Cell Biology 2023Quote: ... using TransIT-293 transfection reagent (Mirus). Lentivirus was harvested 48 hours after transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were transfected using TransIT-LT1 (Mirus Bio LLC, Madison, WI) with 0.75 μg third-generation lentiviral packaging mix (pMD2.G (Addgene #12259) ...
-
bioRxiv - Developmental Biology 2023Quote: ... we transfected S2 cells with pAC5.1-mcd8-CIBN-NTEV-NICD-GFP and pAC5.1-Cry2-mCherry-CTEV using the TransIT-Insect Reagent (Mirus, MIR #6104) according to manufacturer’s recommendation ...
-
bioRxiv - Cell Biology 2023Quote: ... and pMDLg/pRRE) at a 1:1 ratio and transfected into HEK293T cells using TransIT LT1 transfection reagent (Mirus Bio LLC). After 72 h virus-containing supernatants were collected ...
-
bioRxiv - Bioengineering 2023Quote: ... The DNA mixtures were mixed with TransIT-X2 (Mirus) at a ratio of 0.5 µL of TransIT-X2 per 100 ng of total DNA ...
-
bioRxiv - Bioengineering 2023Quote: ... TransIT-LT1 (Mirus Bio) was used as transfection reagent according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors were produced in HEK-293T cells by co-transfection of the previously sequenced vector constructs with psPAX and pMDG.2 packaging plasmids using Trans-IT LT-1 (Mirus, MIR2700). HEK293T cells were incubated overnight at 37°C and the next day medium was changed for IMDM 10% HI-FBS ...
-
bioRxiv - Biophysics 2023Quote: ... and 100 ng of the plasmid of interest were transiently co-transfected using 1.875 µL TransIT (Mirus Bio), and Opti-MEM (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... HEK293T cell line was used for retroviral and lentiviral system amplification with TransIT-293 Transfection Reagent (Mirus).
-
bioRxiv - Cell Biology 2023Quote: ... the samples were washed with 2 × SSC three times and then the nucleic acids in the samples were amine-modified using a Label IT Amine modifying kit (Mirus Bio) for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... 48 μl of TransIT-LT1 (Mirus Bio LLC) reagent was mixed into 1.3 ml Opti-MEMTM (Thermo Fisher Scientific ...