Labshake search
Citations for PacBio :
801 - 850 of 1293 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... we de novo assembled the genomes of 10 samples in this study sequenced by PacBio HiFi reads and then extracted NRSs (Table S3) ...
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bioRxiv - Genomics 2022Quote: ... and were sequenced using the Pacific Bioscience (PacBio) RSII (n=40) ...
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bioRxiv - Microbiology 2022Quote: To generate VSV-G-pseudotyped viruses (for functional selection and long-read PacBio sequencing) from cell-stored deep mutational scanning libraries we plated 60 million library-containing cells per 3-layer flask (Corning Falcon 525cm2 Rectangular Straight Neck Cell Culture Multi-Flask ...
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bioRxiv - Microbiology 2022Quote: Genome-wide methylation motifs on LS1 DNA were identified by PacBio sequencing of genomic DNA harvested from LS1 ΔRM1 (to identify the RM2 methylation motif ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were prepared following the protocol “Procedure & Checklist – Preparing HiFi SMRTbell® Libraries from Ultra-Low DNA Input” (PacBio, CA, USA) and subsequently inspected using a FEMTOpulse ...
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bioRxiv - Zoology 2022Quote: HMW DNA was used for library construction and sequencing on a Sequel II instrument using HiFi mode (PacBio). A target of ~20 kb fragment size was used to construct SMRTbell libraries using CCS Express Library Kit V2 ...
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bioRxiv - Plant Biology 2022Quote: ... from PacBio Sequel bam files were aligned on the draft assembly ...
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bioRxiv - Plant Biology 2022Quote: ... a run movie time of 600 min and Software v6.0 (PacBio).
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bioRxiv - Genomics 2022Quote: ... The constructed library was then sequenced by Pacbio Sequel II platform according to the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... USA) to eliminate fragments <20 kb and sequenced with a Four SMRT Cell 8M on the Sequel II system (PacBio). The sequence reads were converted into HiFi reads with the ccs pipeline (PacBio ...
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bioRxiv - Evolutionary Biology 2022Quote: A genome assembly was initially generated de novo from PacBio reads from X’X females ...
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bioRxiv - Evolutionary Biology 2022Quote: ... To further increase the read quality and coverage we applied the tool DeepConsensus (v0.2, on PacBio reads within 98.0-99.5% read accuracy)38 and gained an overall yield of 30.02 Gb (N50 ...
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bioRxiv - Microbiology 2022Quote: ... A ready-to-sequence SMRTBell Polymerase Complex was created using a Binding Kit 2.2 (PacBio) and the primer V5 ...
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bioRxiv - Microbiology 2022Quote: ... The complete genome was generated based on long read sequencing on the Pacific Biosciences (PacBio) Sequel platform ...
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bioRxiv - Microbiology 2022Quote: ... suecica CCMP904 (IFREMER version) was performed using long read sequencing technologies (PacBio and ONT, respetively), which resulted in longer assembled contigs ...
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... This dual-enzyme hybrid scaffolding used the Bionano optical maps to scaffold polished (PacBio and Illumina) contigs.
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... with 16 threads of freebayes running in parallel across regions of 100kb in the de novo polished genome assembly (PacBio, Illumina and Bionano). Single nucleotide polymorphisms (SNPs ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation and Pacific Biosciences (PacBio) sequencing using Sequel® Sequencing Kit 3.0 (Cat # 101-613-700 ...
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bioRxiv - Plant Biology 2022Quote: ... and CCS reads were generated using SMRTLink v10.0 (PacBio). The obtained BAM file was processed using IsoSeq v3.4.0 pipeline ...
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bioRxiv - Plant Biology 2022Quote: ... the reads were sequenced using the Sequel IIe instrument (PacBio), and CCS reads were generated using SMRTLink v10.0 (PacBio) ...
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bioRxiv - Plant Biology 2022Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Thereafter ...
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bioRxiv - Plant Biology 2022Quote: ... Library preparation was performed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA), and reads longer than 20 kb were collected using BluePippin (Sage Science ...
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bioRxiv - Plant Biology 2022Quote: ... the libraries were sequenced using Sequel IIe instrument (PacBio), and HiFi reads were selected from the circular consensus reads generated.
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bioRxiv - Cell Biology 2022Quote: A reference fasta file was generated with the 5 picked candidates from PacBio sequencing dataset ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were then sequenced on the PacBio Sequel platform (PacBio, USA). The N50 of PacBio reads were from 16.4 to 21.9 kbp ...
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bioRxiv - Bioengineering 2022Quote: ... The Amplicon Template Preparation and Sequencing from PacBio(56) was used to prepare the DNA for long-read sequencing ...
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bioRxiv - Immunology 2022Quote: ... 16-nucleotide barcodes were associated with their BA.1 variants by PacBio sequencing ...
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bioRxiv - Microbiology 2022Quote: ... the quantity and size of each library was assessed and sequenced on a Pacific Biosciences (PacBio) RSII instrument using P6-C4 chemistry with 6 h movie time following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: The taxonomic composition of bacterial communities in the post-mortem human brain tissues were analyzed using the Pacific Biosciences (PacBio) single molecule real-time (SMRT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a diploid assembly of 931.9Mb with a N50 of 1.02Mb that was subjected to polishing with the Arrow algorithm (v2.3.2) as implemented in the GenomicConsensus package (PacBio) after aligning back the raw PacBio reads with Pbmm2 (v1.1.0 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... these were found to be due to indels introduced by PacBio sequencing ...
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bioRxiv - Genomics 2022Quote: ... we downloaded 167 Gb of raw read data (30× consensus read depth, generated with the PacBio Sequel instrument). Since the ONT base-calling is under constant development ...
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bioRxiv - Genomics 2022Quote: Circular consensus sequences (CCS) were first called from PacBio data using ccs version 3.4.1 (https://ccs.how/) ...
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bioRxiv - Molecular Biology 2022Quote: Pac-bio long-read sequencing library was done with the SMRTbell Express Template Prep Kit 2.0 (Pacbio) with the Iso-seq protocol as described by manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing Kit 2.0 (PacBio, 101-389-001) and Instrument Chemistry Bundle Version 11.0 were used ...
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bioRxiv - Cancer Biology 2022Quote: ... using the SMRTbell Enzyme Clean-Up Mix (PacBio, 101-932-600). Library construction typically yielded sufficient SMRT bell library (∼ 70 ng ...
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bioRxiv - Genomics 2022Quote: ... The sheared DNA was used for HiFi SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA). The resultant library was separated on BluePippin (Sage Science ...
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bioRxiv - Genomics 2022Quote: We analyzed LR-RNA-seq data from a range of protocols and platforms (cDNA and CapTrap for PacBio; dRNA, R2C2, and cDNA CapTrap for ONT) from LRGASP [25] with LAPA ...
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bioRxiv - Genomics 2022Quote: ... DNA library construction was performed with the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The fractionated DNA libraries were sequenced on SMRT cells on the Sequel II and Sequel IIe system (PacBio). HiFi reads were constructed with the CCS pipeline (https://ccs.how).
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bioRxiv - Genomics 2022Quote: ... (c) CAGE support of TSS of transcript isoforms constructed from PacBio cDNA protocol ...
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bioRxiv - Genomics 2022Quote: (a) Quantseq3 support of TES of transcript isoforms constructed from PacBio cDNA protocol with TALON before and after transcript end site correction with LAPA ...
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bioRxiv - Genomics 2022Quote: ... It’s recommended to use the barcode kit by PacBio to achieve the best sequencing result ...
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bioRxiv - Genomics 2022Quote: ... Barcoded PacBio SMRTBell adapters from the PacBio 8A barcoded adapter kit (Barcoded Overhang Adapter Kit - 8A, PacBio PN: 101-628-400) were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020) ...
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bioRxiv - Genomics 2022Quote: ... were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020)) using the PacBio SMRTbell Express Template Prep Kit 2.0 (PacBio, PN: 100-938-900) as follows ...
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bioRxiv - Genomics 2022Quote: ... The PacBio SMRTbell library (~20kb) for PacBio Sequel was constructed using SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol ...
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bioRxiv - Genomics 2022Quote: ... The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II ...
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bioRxiv - Genomics 2022Quote: ... using SMRTbell Express Template Prep Kit 2.0 (PacBio, #100-938-900) and sequenced on PacBio Sequel II system ...
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bioRxiv - Genomics 2022Quote: ... resulted in a much more contiguous draft genome assembly compared to other grape genomes assembled with older long-read sequencing technologies despite a lower X-Fold coverage employed (PacBio Sequel CLR reads for M ...