Labshake search
Citations for Promega :
4501 - 4550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Luciferase activity was measured with addition of 50 μL Steady-Glo (Promega cat #E2510), incubated for 20 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Endogenous UDP release was detected by UDP-GloTM Glycosyltransferase Assay kit (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... Endogenous UDP release was measured by UDP-GloTM Glycosyltransferase Assay kit (Promega). HDAC activity was detected by HDAC Cell-Based Activity Assay kit (#600150 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured by CellTiter-Blue® Cell Viability Assay kit (Promega). Endogenous UDP release was detected by UDP-GloTM Glycosyltransferase Assay kit (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... Cell number was measured by CellTiter-FluorTM Cell Viability Assay kit (Promega). Endogenous UDP release was measured by UDP-GloTM Glycosyltransferase Assay kit (Promega) ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted (Maxwell 16 LEV simplyRNA purification kit, Promega, Madison, WI), and libraries were prepared and sequenced using standard protocols and a Nextseq 2000 instrument (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: ... CDC20 and ANAPC4 knockouts were obtained with sgRNA plasmids transfected using FuGENE® HD Transfection Reagent (Promega, E2311), followed by selection using puromycin (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... using the Dual Luciferase® Reporter Assay System (Promega, Madison, WI, USA) and the VICTOR™ X Multilabel Plate Reader (PerkinElmer ...
-
bioRxiv - Cancer Biology 2023Quote: To generate the pmiR-6741-F1 and pmiR-6741-F2 constructs used for promoter validation in the pGL3-Basic vector (Promega, Madison, WI, USA), fragments were amplified using specific primers and human genomic DNA as the template and cloned using a standard laboratory method (S5 Table) ...
-
bioRxiv - Biophysics 2023Quote: ... or Janelia Fluor® 635 HaloTag Ligand (MNRs) (Cat. No. CS315103, Promega) or Janelia Fluor® 646-BAPTA-3’AM-HaloTag ligand (requested from Lavis lab ...
-
bioRxiv - Biophysics 2023Quote: ... each respective cell type was incubated at 37 °C with 500 pM of Janelia Fluor® 646 HaloTag Ligand (NSCs, ECs, or keratinocytes) (Cat. No. GA1120, Promega) or Janelia Fluor® 635 HaloTag Ligand (MNRs ...
-
bioRxiv - Cell Biology 2023Quote: ... we co-transfected 100 ng of pGL4-Dusp13 or pGL4-Dusp27 with 50 ng of pRL-TK vectors (Promega) and varying amounts (0 ...
-
bioRxiv - Cell Biology 2023Quote: ... Luciferase activity was measured using the GloMax® 20/20 Luminometer (Promega) and normalized based on Renilla luciferase activity from the pRL-TK vector.
-
bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and assayed using the Dual-Luciferase Reporter Assay System (Promega). Luciferase activity was measured using the GloMax® 20/20 Luminometer (Promega ...
-
bioRxiv - Cell Biology 2023Quote: ... using the FuGENE HD transfection reagent (Promega). Twenty-four hours after transfection ...
-
bioRxiv - Neuroscience 2023Quote: ... The cDNA was transcribed from 300 ng total RNA using the reverse transcription system (Promega, USA) in a final volume of 50 μl ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All the resulting amplicons were purified using the Wizard SV PCR cleanup system (Promega). The DNA fragment for the vector was amplified by PCR using the primer set Inv-pARP3-fwd/Inv-pARP3-rev and the pARP3 plasmid as the DNA template ...
-
bioRxiv - Synthetic Biology 2023Quote: ... After the purification using Wizard SV PCR cleanup system (Promega), 0.18 pmol of the insert DNA fragment and 0.02 pmol of the vector DNA fragment were directly electroporated into REK13 mutant cells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The extracted plasmid was linearized by SalI digestion and purified using the Wizard SV PCR cleanup system (Promega, Madison, WI). SMRT sequencing was performed using Sequel IIe (Pacific Biosciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Dual Luciferase Reporter Assay kit from Promega. Firefly luciferase activity was normalized against Renilla luciferase activity.
-
bioRxiv - Molecular Biology 2023Quote: ... SYBRgreen-based GoTaq 1-Step qRT-PCR System (Promega #A6020) and LightCycler 96 software was used for RNA quantification ...
-
bioRxiv - Molecular Biology 2023Quote: ... one of these plasmids is combined with a non-targeted firefly luciferase reporter pGL4-SV40 (Promega; for simplicity referred to as FL) and a Renilla luciferase reporter carrying a Mos cognate sequence complementary to MosIR-expressed dsRNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were transfected with FuGENE® HD Transfection Reagent (Promega, Woods Hollow Road, Madison, USA) following the commercial protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... inner salt] assay (Promega, UK). After the incubation periods ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were pretreated with RQ1 RNase-Free DNase (Promega, USA). The reverse-transcription reaction was performed by RNAscribe RT (Biolabmix ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... and then cells were transfected with the fragments which were cloned into the pGL4.10 luciferase reporter basic vector (E1751, Promega, USA), and the PRL-TK reilla luciferase reporter constructs were used as an internal control for plasmid transfection efficiency ...
-
bioRxiv - Neuroscience 2023Quote: ... The luciferase activity was then determined using a dual-luciferase assay report kit (E1910, Promega, USA) by a Microplate reader in accordance with the manufacturer’s instructions after transfection for approximately 24 hours.
-
bioRxiv - Neuroscience 2023Quote: ... potassium Salt (Promega, Cat# E1603) and a microplate Luminometer (Berthold technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega) in accordance with manufacturer instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... FuGENE HD (Promega-E2313), mouse TNF-α ELISA kit (Invitrogen-88-7324-88) ...
-
bioRxiv - Neuroscience 2023Quote: ... Dual Luciferase Reporter Assay System (Promega) was used to measure the intensities of Gaussia and Firefly luciferases ...
-
bioRxiv - Microbiology 2023Quote: ... by using the Glomax Navigator Microplate Luminometer (Promega). The ratios of firefly luciferase to Renilla luciferase activities were calculated for each sample ...
-
bioRxiv - Microbiology 2023Quote: ... and pTK-renilla luciferase plasmid was from Promega.
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37 °C for 24 h and then they were washed with 200 μL PBS per well and harvested in 100 μL per well of passive lysis buffer (Promega). Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo ...
-
bioRxiv - Microbiology 2023Quote: ... Measurement of luciferase activity was carried out using a Glomax Navigator Microplate Luminometer (Promega). In all experiments ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo, Promega), and 10 μL of each lysate was added to 50 μL of 2 μg/mL Renilla luciferase substrate (NanoLight Technology) ...
-
bioRxiv - Microbiology 2023Quote: ... Luciferase activity was measured 36 h post-transfection using the Dual-Glo luciferase assay system (Promega) by using the Glomax Navigator Microplate Luminometer (Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... The Renilla reporter plasmid pRL-TK (Promega) was co-transfected as a control for transfection efficiency ...
-
bioRxiv - Molecular Biology 2023Quote: ... A volume of cells equivalent to 1 mL at OD600 = 0.2 was centrifuged and re-suspended in 1ml fresh medium supplemented with JF549 (Janelia Fluor®HaloTag®Ligands, Promega) at a final concentration of 1 µM ...
-
bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... and RNase inhibitor (Promega) in DEPC treated water ...
-
bioRxiv - Molecular Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE 6 (Promega), Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... and pAdvantage (Promega). Media containing retrovirus was collected 48 h and 72 h after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... HaloTag Amine (O2) Ligand (Promega; P6711) was dissolved in dimethylformamide (DMF ...
-
bioRxiv - Molecular Biology 2023Quote: ... FuGENE® HD Transfection Reagent (Promega) was used as per the manufacturers instruction ...
-
bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... and RNase inhibitor (Promega) in DEPC treated water ...
-
bioRxiv - Developmental Biology 2023Quote: ... GSH measurements were performed using GSH-Glo™ Glutathione Assay kit (Promega) according to the manufacture’s instruction and SperctraMax L (Molecular Devices ...