Labshake search
Citations for Promega :
4451 - 4500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 1 μL of 10 mM dNTPs (Promega, cat. no: U1515), and 0.5 μL of Phusion Polymerase (NEB ...
-
bioRxiv - Neuroscience 2023Quote: ... MTS reagent (Promega) was added to neurons 1:5 ratio to medium volume at DIV11 according to manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: Plasmid DNA is prepared using the Wizard SV 96 Plasmid DNA Purification System (Promega) and the concentration is measured by fluorescence-based quantification (Qubit dsDNA BR Assay Kit ...
-
bioRxiv - Synthetic Biology 2023Quote: ... then adding 30 μl of pre-mixed nGlo buffer with the recommended substrate concentration (Promega, #N1110). Luminescence was read in black opaque 96 well plates (Thermo Scientific ...
-
bioRxiv - Genomics 2023Quote: ... DNA yield was determined using the QuantiFluor dsDNA System (Promega). Samples were subjected to quality control by multiplex PCR for four genomic loci on different chromsomes ...
-
bioRxiv - Plant Biology 2023Quote: ... Extracted RNA was treated using RQ1 RNase-Free DNase (Promega) to eliminate genomic DNA contamination ...
-
bioRxiv - Plant Biology 2023Quote: ... The plasmid DNA was extracted using the PureYield™ Plasmid Midiprep System (A2492, Promega, USA), followed by vacuum concentration in a SpeedVac Concentrator (Thermofisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems) ...
-
bioRxiv - Immunology 2023Quote: ... 10µg of recombinant C5AP and PRGA was trypsinized with 1µg of trypsin (Sequencing Grade Modified Trypsin, Promega) for 15 min at 37°C and the trypsin activity was inhibited by incubating at 100°C for 5 min ...
-
bioRxiv - Systems Biology 2023Quote: ... 4 μL of digestion buffer containing 20 ng/μL sequencing-grade trypsin (Promega), 20 ng/μL sequencing-grade Lys-C (Wako) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 0.5 U μl-1 RNAsin (Promega)] ...
-
bioRxiv - Systems Biology 2023Quote: The transfections were performed with a 1 mg DNA: 2 ml Fugene HD (Promega E2311) ratio ...
-
bioRxiv - Cancer Biology 2023Quote: Cell growth and survival was measured using a CellTiter 96® Non-Radioactive Cell Proliferation Assay at an absorbance of 570 nm using (Promega, Mannheim, Germany). Each assay was performed in triplicates and repeated at least 3 times ...
-
bioRxiv - Microbiology 2023Quote: ... LDH release was measured within supernatants using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) per manufacture’s protocol ...
-
bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
-
bioRxiv - Microbiology 2023Quote: ... and GoTaq (Promega, Madison, WI, USA) polymerases were used according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
-
bioRxiv - Microbiology 2023Quote: ... gel using the Wizzard SV Gel and PCR clean system (Promega, USA) and quantified with Qubit dsDNA HS assay (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... The plate was read with a plate reader (GloMax® Discover Microplate Reader (Promega) or CLARIOstar Plus Microplate Reader (BMG Labtech) ...
-
bioRxiv - Microbiology 2023Quote: ... it was rehydrated with a solution containing 0.5 mg trypsin (Promega corp., Madison, WI, USA) in 20 mM ammonium bicarbonate ...
-
bioRxiv - Microbiology 2023Quote: Firefly luciferase activity was detected using the Luciferase Assay System (#E4550; Promega, Madison, WI). At 3 days post-transduction for Hela cells or 5 days post-transduction for HAE-ALI cultures ...
-
bioRxiv - Microbiology 2023Quote: ... and cells were lysed in 200 µL per 48-well of Lysis Buffer (Promega), then frozen at −80C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
-
bioRxiv - Microbiology 2023Quote: ... 200 µl elution buffer (1 M urea, 100 mM NH4CO3, 1 µg Trypsin (Promega)) was added to the beads and incubated at 1000 rpm on a thermomixer at 27°C for 45min ...
-
bioRxiv - Microbiology 2023Quote: ... centrifuged and the supernatant was measured using the dual luciferase reporter kit (Promega, E1980) with a luminometer (Berthold Centro LB960 microplate reader ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was then extracted and purified using the Wizard Genomic DNA kit (Promega, UK). Purified DNA was Illumina sequenced at the Quadram Institute ...
-
bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were cleaned using a gel extraction kit (Promega) according to the manufacturer’s protocol and used for cloning using a Gibson assembly protocol ...
-
bioRxiv - Microbiology 2023Quote: ... using the LDH-Glo™ Cytotoxicity assay (Promega) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... using the 3D CellTiter-Glo Luminescent cell viability assay (Promega) as published previously (Wrzesinski et al. ...
-
bioRxiv - Microbiology 2023Quote: ... at which point 20 μL of CellTiter 96 Aqueous Non-Radioactive cell proliferation assay (Promega) was added to each well ...
-
bioRxiv - Microbiology 2023Quote: ... and firefly luciferase activity was determined using the Luciferase Assay Kit (Promega #E1501) according to the manufacturer’s instructions on an Orion microplate luminometer (Berthold).
-
bioRxiv - Microbiology 2023Quote: ... the Renilla Luciferase Assay System (Promega, UK) was used according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and Renilla luciferase activity was measured using a GloMAX® Explorer microplate reader (Promega, UK).
-
bioRxiv - Microbiology 2023Quote: ... Pellets were dissolved in 300 µl 1x Glo Lysis buffer (Promega) and incubated for 10 min at RT ...
-
bioRxiv - Microbiology 2023Quote: ... and 50 µl cleared lysate was mixed with 50 µl Bright Glo reagent (Promega) for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega ...
-
bioRxiv - Microbiology 2023Quote: ... for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega) for determination of cell viability/ATP concentration ...
-
bioRxiv - Microbiology 2023Quote: ... were used with the GoScript™ Reverse transcriptase (Promega) according to the manufacturer instruction.
-
bioRxiv - Microbiology 2023Quote: ... and hot-start Taq (Promega) in a buffer of 20 mM Tris-Cl pH 8.3 ...
-
bioRxiv - Microbiology 2023Quote: ... treated with RQ1 RNase-Free DNase (Promega), and then phenol-extracted ...
-
bioRxiv - Immunology 2023Quote: ... before being subjected to a dual-luciferase assay (Promega, Madison, WI, USA) following the supplier’s protocol on a VICTOR X3 plate reader with injector (PerkinElmer ...
-
bioRxiv - Molecular Biology 2023Quote: ... GoTaq DNA polymerase and reagents (Promega, USA) were used to amplify the products ...
-
bioRxiv - Molecular Biology 2023Quote: ... pGemT-vector (Promega). We amplified the left homology arm (637 bp ...
-
bioRxiv - Molecular Biology 2023Quote: ... JF549 (Promega, GA1110) was added to cells using an Echo acoustic liquid dispenser to a final concentration of 25 pM ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 μM Halo-PROTAC3 (Cpos; Promega, GA3110) and DMSO (Cneg ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli BL21 (DE3) pLysS (Promega, WI, Madison, USA) strain harboring the plasmid pAflRT was grown to OD600 = 0.3 in LB medium with 25 μg/mL kanamycin at 37 °C ...
-
bioRxiv - Immunology 2023Quote: ... synthesized using an AMV Reverse Transcriptase kit (Promega, Madison, WI), were amplified using SYBRGreen Power UP Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... TnT® T7/SP6 Quick Coupled Transcription/Translation System (L1170/L2080) (Promega).
-
bioRxiv - Microbiology 2023Quote: ... 0.2 mM PCR Nucleotide Mix (Promega) 10 μM of each primer ...
-
bioRxiv - Microbiology 2023Quote: PCR products and a standard ladder (Promega) were electrophoresed on 1% agarose gels in 1X TBE for 1 hr at 85V ...
-
bioRxiv - Microbiology 2023Quote: ... coli and isolated using the PureYield™Plasmid Midiprep System (Promega) according to the manual.