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Citations for Promega :
4351 - 4400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR was performed with GoTaq qPCR Master Mix (Promega, A6002) and a Quantstudio5 Real-Time PCR Detection System (ThermoFisher) ...
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bioRxiv - Physiology 2023Quote: ... Luminescence was recorded using CellTiter-Glo® 2.0 reagent (G9242, Promega) according to the manufacturer’s instructions using white ...
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bioRxiv - Physiology 2023Quote: ... purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned into the vector pCR2.1 using the TOPO TA Cloning Kit (ThermoFisher ...
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bioRxiv - Physiology 2023Quote: ... Lactate production was measured with the Lactate-Glo™ Assay (Promega) according to the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... which automatically adds 100 μL of 1x Luciferase Assay Reagent (Promega) to the sample and subsequently measures the luminescence ((background ...
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bioRxiv - Plant Biology 2023Quote: ... The SV Total RNA Isolation System (Promega, Madison, USA), which incorporates a DNase treatment step ...
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bioRxiv - Plant Biology 2023Quote: ... emission wavelength MU: 455 nm) using a fluorometer (Glomax-Multi+ Detection System with InstinctR Software, Promega). The promoter activity was determined by calculating the relative LUC/GUS ratio.
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bioRxiv - Plant Biology 2023Quote: ... and each slab was subjected to in-gel proteolytic digestion with trypsin (Promega, Mannheim, Germany) overnight as described in (Shevchenko et al. ...
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bioRxiv - Plant Biology 2023Quote: ... sprayed with 1 mM D-luciferin (Promega) and kept in dark for 10 min before detection by using ChecmiDoc MP machine (BioRad).
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bioRxiv - Plant Biology 2023Quote: ... RNA samples were treated with RNase-free RQ1 DNase (Promega, Charbonnières-les-Bains, France) for removal of DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... and reversely transcribed with GoScript reverse transcriptase (Promega). The qPCR was performed using SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was thereafter treated with RNase-Free DNase (Promega) and reverse transcribed (1 μg ...
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bioRxiv - Plant Biology 2023Quote: ... into Gateway compatible modified pTnT vectors (Promega) containing an N-terminal HA or FLAG tag as specified in each case ...
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bioRxiv - Synthetic Biology 2023Quote: Detection of the cAMP was performed using a commercial luciferase-based kit (#V1501, Promega) in 384-well format using cell lysates according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
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bioRxiv - Plant Biology 2023Quote: ... and the ratio of Firefly luciferase to Renilla luciferase was measured and calculated by using a luminometer (GloMax 20/20, Promega), with a Dual-Luciferase Reporter Assay System (E1910 ...
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bioRxiv - Plant Biology 2023Quote: ... 1% [v/v] Triton X-100) (Promega) was added to the harvested protoplasts for lysis ...
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bioRxiv - Plant Biology 2023Quote: ... with a Dual-Luciferase Reporter Assay System (E1910, Promega), according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... with a modified protocol as written for Promega Nano-Glo Live Cell Assay System (Promega cat#N2011). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was mixed with 100 μl luciferase substrate (Bright-Glo Luciferase Assay System, purchased from Promega, USA), and luminescence was measured immediately by the GloMax Navigator System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... The transfected cells were treated with anthracyclines or Sendai virus (40 hemagglutinin [HA] units/ml) for 24 hours before luciferase activity was determined with the Steady-Glo luciferase assay system (Promega, cat. E2520).
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then processed to measure NAD+ and NADH levels using the NAD/NADH-Glo assay (Promega, G9071) per the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... DNA was then purified using a Promega PCR Clean-Up Kit (Promega #A9282), quantified (Synergy HTX ...
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Neuroscience 2023Quote: ... to an agar block (4% agarose, low melting point, analytical grade, Promega, Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... we used co-transfection experiments with the reporter plasmid pGL4.30 (Promega) that contains upstream to the Firefly luciferase sequence 3x a binding sequence for NFAT ...
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bioRxiv - Neuroscience 2023Quote: ... the luminescence detection assay was performed following all the manufacturer’s recommendations (Dual-Luciferase Reporter Assay Kit System E1910 (Promega).
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bioRxiv - Neuroscience 2023Quote: ... cytoTox-ONE™ Homogeneous Membrane Integrity Assay (Promega®) was used ...
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bioRxiv - Neuroscience 2023Quote: Four to five organoids were physically lysed using a pipette and purified for RNA on the Promega Maxwell RSC48 in conjunction with the Maxwell RSC Simply RNA Tissue kit (Promega, AS1340). cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... using GoTaq qPCR Master Mix (Promega). Primers used had an efficiency level between 85% and 110% ...
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bioRxiv - Neuroscience 2023Quote: Probes for in situ hybridization were made by cloning PCR products into pGemTeasy (Promega). 1 μg of the linearized plasmid was transcribed in vitro using NTP labelling mix and T7 or SP6 RNA Polymerase ...
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bioRxiv - Neuroscience 2023Quote: The intracellular [ATP] was determined by lysing brain capillary ECs and quantifying luminescence produced by luciferase-induced conversion of ATP to light using the CellTiter-Glo Assay 3D (Promega, Madison, WI, USA). Approximately ∼500 ECs were added to each reaction ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then diluted with 50 mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Fugene HD (Promega) using DNA:reagent ratio 1:3 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight with Sequencing Grade Modified Trypsin/Lys-C (Promega) at 370C ...
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bioRxiv - Neuroscience 2023Quote: GFP- or Flag-synapsin constructs were separately transfected into HEK 293 cells using Lipofectamine 2000 (Promega). Transiently transfected cells were washed with PBS and lysed in immunoprecipitation buffer (20 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were digested in two steps with LysC/trypsin (Promega). After overnight trypsin digestion in 2 M Urea ...
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bioRxiv - Neuroscience 2023Quote: ... After overnight digestion using Trypsin/Lys-C (Promega, #V5073), supernatant was collected from the magnetic beads ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then diluted with 50 mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested with LysC (Promega #VA1170) at 1:30 (enzyme:protein ...
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bioRxiv - Neuroscience 2023Quote: ... trypsin (Promega, Cat# V5280), and Lys-C (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... sequencing-grade trypsin (Promega, Madison, WI, USA) was added at a weight ratio of 1:20 (trypsin:protein ...
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bioRxiv - Neuroscience 2023Quote: ... for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega, Cat# V5280), at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Neuroscience 2023Quote: ... The same volume of 0.2 % ProteaseMAX (Promega, Cat# V2072) solution in ABC buffer was added and incubated for an hour with vortex ...