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Citations for Promega :
4301 - 4350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... luciferase was measured on a plate reader using the bright-glo luciferase reagent (Promega).
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bioRxiv - Microbiology 2023Quote: ... neutralization was measured as indicator of luciferase activity in the cells (relative luminescence unit) by using nano-Glo luciferase substrate (Promega Inc.). Luminescence was measured by using Cytation-5 multimode reader (BioTech Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... The infection was measured by assaying activity of intracellular luciferase enzyme by using nano-go luciferase system (Promega).
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated for 48 hours at 37°C in a CO2 incubator and luciferase activity was quantified by adding nano-glo luciferase substrate (Promega). The luminescence was recorded by using Cytation 5 plate reader (BioTek inc) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase production was measured with the Dual-Luciferase® reporter assay system (Promega, #E1980) at a Tecan Infinite® 200 Pro microplate luminometer (Tecan ...
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bioRxiv - Molecular Biology 2023Quote: ... is commercially available (#E2241, Promega, Walldorf, Germany). pGL3basic-IFNβ-Luc (mIfnb1- FLuc ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of transcripts was performed using the GoTaq® qPCR Master Mix (#A6002, Promega) on a LightCycler 96 instrument (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then digested by Lys-C (Princeton Separations) and trypsin proteases (Promega) (Florens et al. ...
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bioRxiv - Microbiology 2023Quote: ... according to the manufacturer’s specifications (Promega). Reactions were carried out in a MyGene™ thermal cycler (Model MG96G) ...
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bioRxiv - Microbiology 2023Quote: We performed Cell-Titer Glo Luminescent Assay (Promega) as indicated by manufacturer for assessing viability and intracellular ATP level ...
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bioRxiv - Microbiology 2023Quote: We performed NAD/NADH Glo Luminescent Assay (Promega) as indicated by manufacturer for measuring intracellular NADH level ...
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bioRxiv - Microbiology 2023Quote: ... and treated with 1 unit of RNase-free DNase (Promega) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI). HRP conjugated anti-beta antibody (sc-47778 ...
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bioRxiv - Microbiology 2023Quote: ... 1×106 Sf9 insect cells were transfected with bacmid DNA using Fugene (Promega) and cultivated for three days ...
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bioRxiv - Microbiology 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described1 ...
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bioRxiv - Microbiology 2023Quote: ... the permeabilization protocol used a 3 minute 0,02% digitonin (Promega) exposure ...
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bioRxiv - Microbiology 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described1 ...
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bioRxiv - Microbiology 2023Quote: ... Luciferase activity was analyzed by the luciferase assay system (Promega). IC50 was determined by a four-parameter logistic regression using GraphPad Prism 8.0 (GraphPad Software Inc.)
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bioRxiv - Molecular Biology 2023Quote: ... cold lysis buffer with 0.01% Digitonin (Promega), referred to as OMNI lysis buffer (Corces et al ...
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bioRxiv - Molecular Biology 2023Quote: ... Sweden) was engineered by inserting the PGK-NLuc-poly(A) cassette from pNL1.1.PGK (Promega) plasmid into virus genome ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell viability was assessed using the CellTiter 96® AQueousOne Solution Proliferation assay (Promega #G3580). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and analyzed with the PReMiS software (version 1.0.5.1, Promega).
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bioRxiv - Molecular Biology 2023Quote: ... The virus-induced CPE was assessed using the Viral ToxGlo™ assay (Promega #G8943) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase assays to analyze SGR RNA replication was performed using the Renilla luciferase assay system (Promega #E2810) as per the manufacturer’s protocol with the following modifications ...
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bioRxiv - Microbiology 2023Quote: ... The size of the band was confirmed using a 1 Kb ladder (Promega). Sequencing was performed bidirectionally using the respective forward and reverse primers at Macrogen (Korea).
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was incubated with RQ1 RNase-free DNase (Promega) for 45 minutes at 37° C prior use in reverse transcription reactions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) extractions were performed using the Wizard® Genomic DNA Purification Kit (Promega) following pre-treatment of S ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... into the MluI/BglII sites of pGL2-Basic (Promega). The following reagent was obtained through the NIH AIDS Reagent Program ...
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bioRxiv - Molecular Biology 2023Quote: ... The indicated DNA oligonucleotide and oligonucleotide ‘HDVrt’ were mutually extended upon one another using three thermocycles with GoTaq Hot-start DNA polymerase (Promega). This generated a double-stranded DNA comprising the DNA sequence of the desired RNA oligonucleotide ...
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bioRxiv - Molecular Biology 2023Quote: ... NLS-NSD2-mVenus WT and T1150A plasmids were transfected into 70 % confluent HEK293 SETD2 knockout cells using FuGENE® HD Transfection Reagent (Promega). NLS-mVenus empty vector was used as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplicons were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega), followed by Sanger sequencing (Eurofins) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA was ligated with a 5’-hydroxylated ‘Processed Start Site’ (PSS) RNA adaptor oligomers (onkh031) by RNA Ligase 1 (Promega or NEB) and then purified to remove excess oligomers (NEB Monarch RNA Cleanup kit) ...
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bioRxiv - Molecular Biology 2023Quote: ... and were then digested overnight with trypsin (Promega). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼ 5 × 105 U2OS cells were transfected using FuGene6 (Promega) with 1μg of gRNA/Cas9 (px330 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were lysed in 50 µL of 1x passive lysis buffer (Promega) per 96-well at 20 h post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... pGL3basic-IFNβ-Luc (mIfnb1- FLuc) consists of the 812 bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (99) ...
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bioRxiv - Neuroscience 2023Quote: ... and converted to cDNA using the Promega Reverse Transcription system (Promega). SYBR green qRT-PCR was performed in triplicate using cDNA and run on the Bio-Rad CFX384 real time thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... treated with RQ1 Rnase-free Dnase (Promega), and converted to cDNA using the Promega Reverse Transcription system (Promega) ...
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bioRxiv - Physiology 2023Quote: ... whereas HEK293 cells stably expressing a luminescent cAMP GloSensor (GS-293) (Promega) were previously created in our lab 25 ...
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bioRxiv - Physiology 2023Quote: ... The assay medium was supplemented with 2% GloSensor Reagent (Promega #E1291) and 0.1% BSA ...
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bioRxiv - Physiology 2023Quote: ... 25 ng/mL fibroblast growth factor (bFGF; Promega), 1% penicillin streptomycin ...
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bioRxiv - Physiology 2023Quote: ... Proteolysis was performed using trypsin/lysC (Promega, Madison, WI) in 50 mM ammonium bicarbonate overnight at 37°C ...
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bioRxiv - Physiology 2023Quote: ... ATP concentration was measured using a luciferin–luciferase ENLITEN® ATP Assay System (Promega). Luminescence was measured by Infinite M Plex microplate reader (Tecan).
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bioRxiv - Physiology 2023Quote: ... and sequencing-grade trypsin (Promega, Madison, WI, USA) was added at an enzyme-to-protein ratio of 1:50 ...
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bioRxiv - Physiology 2023Quote: ... using Fugene6 (Promega Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The cultured media was replaced with 2 ml of DMEM media supplemented with 200 μM Beetle luciferin (Promega, Wisconsin, Madison). BMAL1Luc HDFs’ bioluminescence was measured continuously with a Kronos Dio Real-time luminometer (Atto ...