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Citations for Promega :
4101 - 4150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was treated with DNase I (Promega) and subjected to reverse transcription using RevertAid Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... + 0.5 μg of “reporter” plasmid (pAluneoTet or other SINE reporter plasmids) + 0.25 μg of pCEP/GFP using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... using 0.01% Protease-MAX surfactant (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... each well was transfected with 1 μg of plasmid DNA (0.5 μg L1 plasmid + 0.5 μg pCEP/GFP) using 4 μL of FuGENE 6 transfection reagent (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... and the chromatin-associated fraction was sonicated on a Bioruptor (5 times 20 sec on/off intervals) prior to DNase treatment using RQ1 DNase (Promega) and RNA extraction using Trizol ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were prepared with Trypsin Gold (Promega) in accordance with manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using trypsin (Promega) at a 1:20 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was transferred to a new tube and digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µl 50 mM ammonium bicarbonate at 37°C for 5 h ...
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bioRxiv - Microbiology 2024Quote: ... 40 U/μL RNAsin (Promega, Fitchburg, WI, USA), 40 mM dNTPs (Bioline ...
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bioRxiv - Microbiology 2024Quote: ... In-solution on-bead digestion of proteins was performed using Immobilized trypsin (Promega, sample-enzyme solution ratio of 94 (v/v) ...
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bioRxiv - Molecular Biology 2024Quote: ... Either 20μL (96 well) or 12μL (384 well) Glo Lysis Buffer (Promega, Cat #E2661) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Luciferase substrate (Promega, Cat #E1501) was prepared according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... x1 RNasin Plus (Promega) and 100 µg/mL cycloheximide) ...
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bioRxiv - Plant Biology 2024Quote: ... One μg of RNA was treated with RQ1 RNase- Free DNase (Promega, M6101) first and then used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... The leaf disc debris was resuspended in 100 µL 1X Passive Lysis Buffer (Promega). The solution was cleared by centrifugation (5 min ...
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bioRxiv - Plant Biology 2024Quote: ... Luciferase quantification was performed according to Dual-Luciferase® Reporter Assay System from Promega recommendation (see link).
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bioRxiv - Cancer Biology 2024Quote: ... total NAD+ and NADH levels and their ratios were determined using NAD+/NADH-glo assay kit (Promega, G9071) as per manufacture’s protocol ...
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bioRxiv - Physiology 2024Quote: Expression plasmids for luciferase and FincoR were mixed with a Coupled Reticulocyte Lysate System (Promega). After incubating at 30°C for 60 min ...
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bioRxiv - Physiology 2024Quote: The enhancer region containing the FXR binding element was amplified by PCR and cloned into the pGL4.23 vector (Promega). HepG2 cells were transiently transfected with FXR (100 ng/well ...
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bioRxiv - Physiology 2024Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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bioRxiv - Microbiology 2024Quote: ... 25 μl of DualGlo reagent (Promega) was added per well ...
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bioRxiv - Molecular Biology 2024Quote: ... and NAD+/NADH ratio by NAD/NADH-GloTM kit (Promega #G9072) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Extracts were validated for transcription using the positive control reagents provided in the commercial HeLaScribe (Promega) kit and subsequent in vitro transcription assays with HepG2 extract were performed as described above for the commercial HeLa extract ...
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bioRxiv - Molecular Biology 2024Quote: PCRs for amplification of chromosomal termini were performed on tailed DNA in 20 µL using GoTaq® Long PCR master Mix (Promega). PCR mix included 10 µL of GoTaq® Long PCR master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... The Fugene 6 reagent (Promega, Madison, USA) was used at a 3:1 rate ...
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bioRxiv - Molecular Biology 2024Quote: ... Mass Spectrometry Grade (Promega, V5280) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in 12-well plates at a density of 200,000 per well and transfected using 1.5 µl/well FuGENE 6 (Promega) with 5 ng of CMV-Renilla plasmid (pTG888) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were analyzed using the Dual-Luciferase® Reporter Assay System (Promega #E1960). Culture medium was aspirated ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using the GoScript Reverse Transcription System (Promega) and quantitative real-time PCR was conducted as described previously (40).
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bioRxiv - Immunology 2024Quote: ... Then reverse transcribed to cDNA using a GoSript Reverse Transcription kit (Promega). Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO ...
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bioRxiv - Immunology 2024Quote: ... and random primers (Promega C118A) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method 23 with Trypsin/Lys-C mix (Promega, Madison, WI, USA) (enzyme-to-protein ratio 1:35 ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was isolated from RLBP1-containing pGEM-T easy colonies and digested with MluI and XhoI (Promega) for 3 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA from the selected clone was purified using the EndoFree Plasmid kit (Promega) and sent to the TaRGet vector core to generate the corresponding AAV-RLBP1 vector (production titres 1.2×1012 vg/ml (pDDO batch in DPBS 1X without CaCl2 and MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated using T4 DNA ligase (Promega) at RT for 1 h into the MluI/XhoI-digested recombinant AAV proviral plasmids pDDO-020-SSV9-CAG-bGHpA or pKL-AAV-CAG-bGHpA (TaRGet vector core ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37 °C with LysC (Wako Chemicals) and trypsin (Promega) in a 1/100 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... and then placed in a 50 mL solution of 1 ×Diamond Nucleic Acid Dye (Promega) in 0.2 ×TBE buffer pH 7.6 to rock in the dark for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cell Titer Glo 2.0 Cell Viability Assay (Promega) was performed on primary cortical neurons following manufacturer instructions including a standard curve from 0.1 nM to 100 μM ATP in each plate for standardization ...
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bioRxiv - Neuroscience 2024Quote: ... 1.5 µg of total RNA was converted into single-stranded cDNA using the ImPromII reverse transcriptase kit (Promega) with random primers (0.5 µg/ml) ...
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bioRxiv - Molecular Biology 2024Quote: ... and PCR was performed using GoTaq® Green Master Mix (Promega). The splicing efficiency is monitored using RT-PCR with primers designed to target the upstream and downstream exons ...
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bioRxiv - Microbiology 2024Quote: ... and the luciferase activity was determined by measuring luminescence in a luminometer (Berthold) after adding luciferin substrate (Promega). Luciferase activity from vector control transfections were compared to transfections with only PKR encoding plasmids to assess the PKR activity for each species ...
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bioRxiv - Microbiology 2024Quote: ... The 5.3-kb product was cloned into pGEM-T Easy (Promega), generating an acuM cloning plasmid ...
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bioRxiv - Biophysics 2024Quote: ... and the Firefly and Renilla luciferase activities were measured using the Dual Luciferase kit (Promega).
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 1ug of plasmid and FuGene 6 (Promega) were used ...
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bioRxiv - Microbiology 2024Quote: ... Fluorescence was detected using GloMax Discover Microplate Reader (Promega).
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bioRxiv - Microbiology 2024Quote: ... the joints were placed in passive lysis buffer (Promega, USA) and mechanically ground with ceramic beads (Precellys ...
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bioRxiv - Biochemistry 2024Quote: ... the amplified PCR products were digested using the corresponding restriction enzymes (Supplementary Table 1) and then ligated into similarly digested pYES2 using LigaFast Rapid Ligation System (Promega Corporation, Madison, WI, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested by DNase I before reverse transcription with murine leukemia virus reverse transcriptase using random primers (Promega). qPCR was performed with and a CFx96 Touch Real-Time PCR system (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... For the analysis of expression of MERVL elements the RNA was additionally digested with RQ1 DNAse (Promega) to guarantee the absence of contaminant genomic DNA ...