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Citations for Promega :
4201 - 4250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The beads were resuspended in 50 mM NH4HCO3 supplemented with trypsin (Mass Spectrometry Grade, Promega; Madison, US) at an enzyme- to-protein ratio of 1:25 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Molecular Biology 2023Quote: ... For ACE2 overexpression HEK293T cells were transfected for 24 h with human ACE2 (pCG1-hACE2, 200 ng DNA/well) using JetPrime (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... whereas amplicons were detected using SYBR Green I (Promega, A6002). Data analysis was carried out by using qBase [11] ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA for microinjection was in vitro synthesized using as a template the plasmids previously linearized with BamHI (Promega) and transcribed using mMESSAGE mMACHINE SP6 kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Chemiluminescence was detected with ECL Western Blotting Substrate (Promega, W1001) using the ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% bovine serum albumin] supplemented with RNAsin (1:1000, Promega, #N2511) for FACS purification ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:1000 RNAsin (Promega, #N2511,) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Renilla-Glo assay substrate (Promega, Cat. #E2750) was dispensed using an automated and customized uHTS Multidrop device (2.5 µl/well) ...
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bioRxiv - Molecular Biology 2023Quote: ... lysed by rocking for 15 min at room temperature in 500 µl of passive lysis buffer (Promega), and clarified by centrifugation for 2 min at 21,000 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... the luciferase expression was assessed (Promega, Cat# E2710) by addition of 3 μl Renilla-Glo™ reagent (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from 293T cells using an Eastep Super Total RNA Extraction Kit (LS1040, Promega, WI, USA). Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell transfection was performed with Fugene HD transfection reagent (E2311, Promega, WI, USA) as described previously (Niu et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The PX459-DNMT1-gRNA plasmid was then used to transfect 293T cells using FuGENE HD Transfection Reagent (E2311, Promega, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by 0.5 µg lysyl endopeptidase (LysC) (Fujifilm-Wako) and 0.5 µg Trypsin (Promega) at room temperature overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 U/mL RNase inhibitor (Promega), Complete Protease Inhibitor EDTA-free in nuclease-free water) ...
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bioRxiv - Molecular Biology 2023Quote: ... or Glu-C (Promega). Next day ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Molecular Biology 2023Quote: ... Bright-Glo (Promega, Madison, USA) was used to monitor the levels of the Luciferase expression at 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Figure 6 and Figure 7) to form condensates in cells was monitored by transient transfection of U-2 OS cells using FuGene (Promega; Figures 2 and 7) or electroporation (as before ...
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bioRxiv - Molecular Biology 2023Quote: The HIV-1C consensus LTR sequence or remainder of gel extracted DNA amplified from LTR U3R of the 41 RNA samples obtained at the earliest post infection timepoint (referred to as T/F LTR) were cloned into the pGL3 Basic vector expressing Firefly Luciferase (Promega Corporation, Wisconsin, USA) as previously described (63) ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were subcloned into pGEM-Teasy (Promega) and then moved as HindIII/NotI fragments into pCB4699 ...
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bioRxiv - Molecular Biology 2023Quote: ... Firefly and renilla luminescences were then monitored with Dual-Glo Luciferase Assay kit (Promega) in a Berthold luminometer ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting product was then digested and inserted between HindIII-KasI sites of pGL3 vector (Promega) giving pBS6286 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells are washed with PBS1X and then lysed with 250 μl of 1X Passive Lysis Buffer (Promega). Firefly and renilla luminescences were then monitored with Dual-Glo Luciferase Assay kit (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... by addition of 3 μl Renilla-Glo™ reagent (Promega, Cat# E2710) and measuring the luminescence signal with the ViewLux uHTS Microplate Imager (PerkinElmer ...
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bioRxiv - Molecular Biology 2023Quote: ... and reverse-transcribed using AMV RT (Promega). cDNA was quantified by qPCR using iTaq Universal SYBR green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... and luminescence was measured on a Tecan M1000 microplate reader using a dual-luciferase assay kit (Promega). Firefly luminescence was normalized to the renilla luminescence to control for each transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... containing RNasin® Plus RNase inhibitor (Promega, N2615) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... and Oligo(dt) 15 primers (Promega, C1101) containing RNasin® Plus RNase inhibitor (Promega ...
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bioRxiv - Molecular Biology 2023Quote: 500 ng of total RNA were converted to cDNA using M-MLV Reverse Transcriptase (Promega, M1705) and Oligo(dt ...
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bioRxiv - Physiology 2023Quote: ... 300 μl aliquots from each fraction after polysomal ultracentrifugation in sucrose density gradients were spiked with 100 pg of Luciferase mRNA (internal control, Promega). Then ...
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bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
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bioRxiv - Neuroscience 2023Quote: ... Trypsin (1:100, Promega, Cat # V5280) was then added for overnight incubation at 37°C with intensive agitation (1000 rpm) ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cells were incubated with media containing MTS reagent (Promega) for two hours before measuring absorbance at 490 nm using a Tecan plate reader.
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bioRxiv - Neuroscience 2023Quote: A pET25 vector encoding the cell penetrating peptide SynB1 underwent restriction digest using BamHI (Promega, catalog #R6021) for 1 hour at 37 °C and SfiI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: PFT-expressing plasmids were purified from an overnight stationary culture of the corresponding strain by using the Wizard Plus SV Minipreps DNA purification System (Promega, cat#A1460) and transformed into E ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell growth was determined using Cell Titer-Glo 2.0 Cell Viability Assay (Promega G9242). IC50 values were calculated in Prism using a four parameter non-linear fit inhibitor vs response model.
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bioRxiv - Neuroscience 2023Quote: ... and read on a Glomax Luminometer (Promega). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... 200 U/mL RNAsin (Promega), 1 mg/mL heparin ...
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bioRxiv - Neuroscience 2023Quote: The PCR was performed with the GoTaq® Green Master Mix (Promega), and the primers used for genotyping were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... Transient transfections of AGS cells were performed using FuGeneHD (Promega) according to the manufacturer’s protocol (using a 3:1 FuGene ...
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bioRxiv - Neuroscience 2023Quote: ... and digested with trypsin (Promega). The resultant peptides were separated by ultra-high pressure liquid chromatography and directly infused into a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... blots were incubated with Enhanced luminol-based Chemiluminescence substrate (Promega), and the exposure was captured using the ImageQuant 800 system (Amersham) ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and luciferase activity was measured on GloMax Discover (Promega). For the reporter assay in infected cells ...
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bioRxiv - Microbiology 2023Quote: ... and then Sequencing Grade Modified Trypsin (Promega) was added at the proportion 1:50 trypsin:protein ratio (w/w ...