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Citations for Promega :
4151 - 4200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the amplified PCR products were digested using the corresponding restriction enzymes (Supplementary Table 1) and then ligated into similarly digested pYES2 using LigaFast Rapid Ligation System (Promega Corporation, Madison, WI, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity of cell lysate was measured using the Nano-Glo Luciferase Assay System (Promega) with a Glomax plate reader (Turner BioSystems).
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bioRxiv - Microbiology 2024Quote: ... On-column digestion occurred using 5 ug trypsin (Promega sequence-grade trypsin) in 50 mM TEAB for 3 hours at 47C ...
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bioRxiv - Plant Biology 2024Quote: ... using GoTaq Long PCR Master Mix (Promega). Construct development was performed as described in Bettgenhaeuser et al. ...
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bioRxiv - Plant Biology 2024Quote: ... HALO-TF protein was subsequently incubated with 10 ug of MagneHALO beads (Promega) for 1 hour rotating at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and 1ug of plasmid DNA was used for in vitro protein expression using the TNT rabbit reticulocyte expression system (Promega) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested by DNase I before reverse transcription with murine leukemia virus reverse transcriptase using random primers (Promega). qPCR was performed with and a CFx96 Touch Real-Time PCR system (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... For the analysis of expression of MERVL elements the RNA was additionally digested with RQ1 DNAse (Promega) to guarantee the absence of contaminant genomic DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and limited digestion by Trypsin/Lys-C Mix (Promega). The samples were then extracted by ethyl acetate ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 400U RNasin Plus inhibitor (Promega Corporation, USA) and 200U SUPERaseIn RNase Inhibitor (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... or 17.6 nM of FFz (Promega). Images were analyzed using Living Image (Perkin Elmer).
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bioRxiv - Synthetic Biology 2024Quote: ... trypsin (Promega, USA) was added to each sample to achieve a NanoLuc to trypsin ratio of 20:1 (w/w ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with DpnI and further purified with a PCR clean-up kit (Promega). The R11 p2-p3 PCR product was then cloned into the pUC57 backbone with Gibson Assembly (50 ng pUC57 backbone and 50 ng p2-p3 insert) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL of DNase I (Promega) was added to the liposomes in order to digest the outer DNA ...
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bioRxiv - Plant Biology 2024Quote: ... Genes of interest were amplified from template DNA by PCR using GOTaq polymerase (Promega) according to the recommendations of the supplier ...
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bioRxiv - Plant Biology 2024Quote: ... the gel-eluted PCR products from T0 lines were cloned into pGEM-T Easy vector (Promega, Germany) or pJET1.2 blunt end vector (Thermo Scientific™ ...
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Olive mill wastewater as a source of by-products promoting plant defense against microbial pathogensbioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s instructions and treated with RQ1 RNase-free DNase (Promega). cDNA was synthesized with ImProm-II™ Reverse Transcription System (Promega) ...
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Olive mill wastewater as a source of by-products promoting plant defense against microbial pathogensbioRxiv - Plant Biology 2024Quote: ... The [Ca2+]cyt detection was performed by GloMax-Multi Detection System (GMDS; Promega Corporation). [Ca2+]cyt was detected by measuring the fluorescence emitted at intervals of 1 minute.
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Olive mill wastewater as a source of by-products promoting plant defense against microbial pathogensbioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized with ImProm-II™ Reverse Transcription System (Promega). qRT-PCR was performed with a CFX96 Real-Time PCR System (BioRad ...
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bioRxiv - Plant Biology 2024Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega) using Clariostar plate reader ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were harvest after 48 hr of agroinfiltration99 and luciferase activity was quantified using the dual-luciferase reporter assay system (Promega). To determine the relative promoter activity ...
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bioRxiv - Plant Biology 2024Quote: ... Trypsin (Promega, Madison, WI, USA) and Lys-C (Wako ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 0.4 µg trypsin (Promega) was added ...
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bioRxiv - Plant Biology 2024Quote: ... using the GoTaq qPCR Mastermix (Promega, USA). Per reaction ...
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bioRxiv - Physiology 2024Quote: ... Insulin and glucagon concentrations were measured by Lumit Immunoassay kit (Promega # W8022).
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bioRxiv - Molecular Biology 2023Quote: ... the nucleocapsid subsequence starting from the start (ATG) of the nucleocapsid ORF to the ending 25-nt poly-A was cloned into a pGEM-7Zf(+) vector (Promega; P2251) by XbaI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800, Promega, WI, USA) according to the manual ...
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bioRxiv - Molecular Biology 2023Quote: ... per manufacturer’s instructions using a GloMax 20/20 luminometer (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... or using the Wizard HMW DNA Extraction Kit (Promega; A2920) following the manufacturer’s protocol with modifications that RNase and Proteinase treatments were each extended to one hour ...
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bioRxiv - Molecular Biology 2023Quote: ... in 384-well plates using the GoTaq qPCR Master Mix (A6002, Promega). Primers were designed by the Primer-BLAST tool (https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi ...
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bioRxiv - Molecular Biology 2023Quote: RNA isolated from cells (1 μg) was digested using the RQ1 RNase-Free Dnase (M6101, Promega) and reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 100 ng Trypsin (Promega, Cat# V5111) overnight digestion at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: RT-PCR was performed using the GoTaq G2 DNA Polymerase (M7845, Promega) with 3 μl of reverse transcriptase product and the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... A single camera was used to capture both Fab (AF488, Thermo-Fisher) and Halo-H2B stained with Halo-ligand (TMR, Promega). A high-speed filter wheel (HS-625 HSFW TTL ...
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bioRxiv - Molecular Biology 2023Quote: ... Streptavidin was purchased from Promega. NusA was a gift from J ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Molecular Biology 2023Quote: ... 100 nM streptavidin (Promega) was included in the master mix ...
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bioRxiv - Molecular Biology 2023Quote: ... each TECprobe-ML reaction was 48 μl due to the omission of 10X (1 μM) streptavidin (Promega) and 10X Start Solution [100 mM MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured after 12 days using CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... 50ul of NAD/NADH-Glo™ Detection Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... Dual luciferase assays were then performed using the Dual Luciferase Assay kit (Promega) as described.(26)
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bioRxiv - Molecular Biology 2023Quote: ... d3 and d4 using colorimetric CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Promega, #G3580) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing grade trypsin was from Promega (Madison WI). HLB Oasis SPE cartridges were purchased from Waters (Milford ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB (pH 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... the HinDIII enzyme was used (Promega Corp., Madison, WI). Reactions were incubated for 60 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Nano-Glo® luciferase assay system (Promega) was used according to manufacturer’s protocol and luminescence was measured for 0.1 second with EnVision® Multimode plate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins were digested overnight with a solution of sequencing-grade trypsin (Promega, San Luis Obispo, CA) in 50 mM TEAB at a 1:50 (wt:wt ...