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Citations for Promega :
3101 - 3140 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The RNA samples (2 μg) were used for the synthesis of cDNA by reverse transcription using GoScript ™ Reverse Transcriptase (Promega, Madison, USA). RT-PCR was performed with a CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Plant Biology 2021Quote: ... The samples after pull-down assay and co-immunoprecipitation were also digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight in the same manner ...
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bioRxiv - Immunology 2021Quote: ... 100 μl of Chloroform were added to 500 μl of homogenized sample and total RNA was extracted using ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae with the same genotype were pooled (min. n=10 larvae/sample) and RNA was extracted using the ReliaPrep RNA Miniprep System (Promega AG, Dübendorf, Switzerland) following manufacturer’s user guide ...
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bioRxiv - Pathology 2021Quote: ... we first prepared cDNAs from a total RNA sample isolated from an RSV-infected rice plant using the Reverse Transcription Kit as instructed (Promega, Madison, WI, USA). We then amplify the full-length RSV RNA3(- ...
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bioRxiv - Genomics 2020Quote: ... Another 1 ml portion of each spiked enrichment sample was extracted using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to manufacturer’s instructions for Gram-negative bacteria with additional RNase treatment ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from FFPE tissue samples using the automated Maxwell system with the Maxwell 16 LEV RNA FFPE Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 100 μl of Chloroform were added in 500 ml of homogenized sample and total RNA was extracted using ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... service by analyzing DNA samples from each individual cell line for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... DNA was eluted with 330 µl 1x TE buffer and 100 µl of DNA samples were pipetted into a 96-well microtiter plate where DNA concentration was determined using QuantiFluor® dsDNA System (Promega Corporation, ZDA) according to the manual ...
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bioRxiv - Cancer Biology 2020Quote: ... CNAs of all samples that were sequenced with the panel 2 were normalized against a reference created from normal samples including the commercial available male (Cat No. G1471) or female (Cat No. G1521) references from Promega (Madison, Wisconsin, US) and DNA from blood of two patients ...
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bioRxiv - Molecular Biology 2022Quote: For high-resolution DNA electrophoresis resolving the nucleosome repeats genomic DNA was purified from chromatin samples using the Wizard SV Gel and PCR Clean-Up System (Promega, catalog no. A9281) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Immunology 2022Quote: ... 100 μl of Chloroform were added to 500 μl of homogenized sample and total RNA was extracted using ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The urea concentration in the lysate was reduced to 2 M with the addition of 50 mM NH4HCO3 and the samples were subjected to overnight trypsin digestion (Trypsin Gold, MS Grade, Promega Corporation, WI, USA). Following digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we digested tissues using Proteinase K enzyme and used a Maxwell® Rapid Sample Concentrator Instrument with Maxwell® 16 Tissue DNA Purification Kit (Promega Corporation). We used 34 cycles polymerase chain reaction (PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method 23 with Trypsin/Lys-C mix (Promega, Madison, WI, USA) (enzyme-to-protein ratio 1:35 ...
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bioRxiv - Molecular Biology 2024Quote: ... was added and samples were digested for two hours at 37°C followed by addition of 1 μg of sequencing-grade Trypsin (Promega, Ref. No. V5113) and digestion overnight at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Cancer Biology 2024Quote: ... Diluted RNA (20 ng/µl) was treated with 10% v/v 10X DNase I Buffer and 10% v/v DNase I (Promega; 15 minutes, 25°C). 10% v/v of RQ1 RNase/DNase Stop Solution (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat. M170A, 1.25µM RT 5X buffer, Promega Cat. M531A, 0.5mg/mL Oligo (dT) 18 Primer ...
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bioRxiv - Biochemistry 2024Quote: ... The 5 mL HEK293T cell suspension was combined with the mixture of 10 µL of Nano-Glo® Live Cell Substrate and 90 µL of Nano-Glo® LCS dilution buffer (Promega, Cat.# N2012). The whole mixture of HEK293T with the Nano-Glo® reagents was added to 96 well plates (Greiner Bio-One ...
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bioRxiv - Biophysics 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® Biotin Ligand (Promega, 200-fold dilution) in PBS supplemented with 5% NGS overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: Messenger RNA was isolated from cells or slices (three slices per sample, pooled, homogenized prior to extraction) using Maxwell® LEV Simply RNA Cells/Tissue kit (Promega, Madison, Wisconsin, US). A NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... One microgram of total RNA from each sample was reverse transcribed into first-strand cDNA by using the M-MLV kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2024Quote: ... The luciferase activity expressed by the recovered sporozoites was assessed (three replicates for each sample) using the Nano-Glo® Luciferase Assay System (Promega Corporation, Madison, USA). The sporozoites were mixed (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Each qPCR reaction contained 2 µL cDNA sample (12.5 ng) and 18 µL mastermix with 1X GoTaq® qPCR Master Mix (Promega, Charbonnières les Bains, France) and 0.5 µM primer ...
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bioRxiv - Microbiology 2024Quote: ... supernatants containing trVLPs were collected and the remaining cells were lysed for luciferase assay using the 5x Promega Passive lysis buffer and luciferase activity was measured using a The Dual-Luciferase® Reporter (DLR™) Assay System (Promega, Madison, WI, USA). After confirming that firefly luciferase values remained consistent between conditions ...
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bioRxiv - Neuroscience 2024Quote: ... The microdissected material was then treated with lysis buffer and stored at - 80°C until RNA extraction using the ReliaPrep™ RNA Cell Miniprep System from Promega (Promega, La Farlede, France). RNA integrity and quantity were assessed using a Bioanalyser 2100 (Agilent Technologies ...
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bioRxiv - Physiology 2023Quote: ... 200 ng of total RNA from each sample was reverse transcribed into cDNA using random hexamer primers and GoScript™ Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). The synthesized cDNA was stored at -20 ℃.
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were washed in the ABC buffer (50mM ammonium bicarbonate, pH 8.3) and proteins digested on beads with TPCK-trypsin (Promega, Madison, WI, 16 hours at 37 °C). The supernatant containing the tryptic peptides was collected and lyophilized ...
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bioRxiv - Genomics 2024Quote: ... pooled PCR fragments > 600 bp in length (300 ng per tube x 2 tubes, each diluted with 100 μl of Promega RQ1 DNase 10x buffer (cat. # M6101)) were lightly digested with 0.2 μl of DNase-I (RQ1 RNase-Free DNase-I ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...