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Citations for Promega :
2651 - 2700 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... cells were harvested in 1× Passive Lysis Buffer and luciferase activities were measured using a Dual-Luciferase Reporter Assay System as per manufacturer’s instructions (Promega, E1980) on a GloMax-Multi plate reader (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then pelleted at 300g for 7 minutes and resuspended in 200μL of Homogenization Buffer from the Maxwell RSC simplyRNA Tissue Kit (Promega #AS1340). Cells were then lysed by adding 200μL of lysis buffer and transferred into the Maxwell RSC Cartridge ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Molecular Biology 2020Quote: ... Cells expressing HT-fusion proteins or HT-Ctrl were incubated in the mammalian lysis buffer supplemented with Protease Inhibitor cocktail (Promega) and RQ1 RNase-Free DNase (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were then lysed with RIPA lysis buffer containing Protease Inhibition Cocktail and RNase Inhibitor followed by DNase treatment (Promega). The immunoglobin-coated protein magnetic beads (Thermofisher scientific ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was removed and pellet was resuspended in 400 μl of sort buffer (1 mM EDTA 0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... NIH3t3 cells were lysed in passive lysis buffer and luciferase activities were quantified using DualGlo luciferase assay system and a GloMax 96 Microplateluminometer (all from Promega). Firefly/Renilla luciferase (FL/RL ...
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bioRxiv - Immunology 2021Quote: ... The pellet then was resuspended in PBS for NTA or 1X Passive Lysis Buffer (PLB) for RNA or protein estimate (Promega). For EV associated RNA and protein ...
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bioRxiv - Plant Biology 2020Quote: ... following the manufacturer’s instructions (eluted into 8 μl of TLE) before A-tailing in a reaction containing 2 μl of GoTaq buffer (Promega), 0.2 μl 10 mM ATP ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Total Drosophila RNA was extracted from Drosophila heads which had been ground to a powder under liquid nitrogen before addition of 250 µl reporter lysis buffer (Promega) and addition of 750 µl PureZOL™ to the frozen lysates to extract the RNA ...
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bioRxiv - Biochemistry 2021Quote: ... FN1-GFP together with pRSV40 Renilla (2% of total transfected DNA) were transiently transfected and lysed in Passive Lysis Buffer (Promega). Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer ...
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bioRxiv - Biochemistry 2021Quote: The samples were reduced and digested in 25 μL co-IP digest buffer (50 mM Tris-HCl, 5 ng/μL trypsin (sequencing grade, modified, Promega), 2 M urea ...
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bioRxiv - Biochemistry 2020Quote: ... three times with 250 μl of 50mM ammonium bicarbonate (ABC) buffer and digested for 16 hours with sequencing-grade modified trypsin (Promega) in 200 μl ABC buffer at 37 °C (enzyme to protein ratio 1:50) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the media was discarded and the wells were washed with PBS before adding 50 μl of Passive lysis buffer (Promega). Cells were frozen at −20°C to facilitate complete lysis.
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bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... at room temperature, with 40 μL assay buffer (CO2-independent medium, 10% FBS) supplemented with 2% (v/v) GlosensorTM reagent (Promega). To stimulate endogenous cAMP via β adrenergic-Gs activation ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500) and placed on a rocker for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody–bead mixture was resuspended in 500 μl of the reaction mixture containing 10 μg of fragmented total RNA,100 μl of reaction buffer and 5 μl of RNasin Plus RNase Inhibitor (Promega), and incubated for at least 4 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... NY) and washed with S-Trap buffer prior to overnight proteolysis at 37°C with LysC/trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Cell Biology 2020Quote: ... gel pieces were reswelled in 60 μL of 50 mM NH4HCO3 buffer containing 10 ng/μL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were treated with the indicated concentration of CHIR99021 24 hours after transfection and after another 24 hours medium was removed and the cells were harvested with 50 µl Passive Lysis Buffer (E1941, Promega). Luciferase activity was measured on a GloMax Navigator (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in passive lysis buffer and Renilla and firefly luciferase activity was measured in duplicates using the Dual-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions in a GloMax 96 Microplate Luminometer (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... samples were diluted 3 times with 50mM Tris buffer (down to 2M final urea concentration) and digested overnight at 37°C using a Trypsin/Lys-C mixture (Promega) at a ratio 1:100 w/w ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were washed three times with 150 μL S-Trap binding buffer and subsequently digested by addition of digestion buffer (500 mM ABC) containing 1:25 (w/w) trypsin (Trypsin Gold, Promega) and incubation at 37°C for three hours ...
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bioRxiv - Microbiology 2022Quote: ... and cells were rinsed in phosphate-buffered saline and lysed by the addition of 40 µl passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Microbiology 2022Quote: ... was used to measure the luciferase activity of each well after the addition of 50-100 µl of reconstituted luciferase assay buffer (Promega). Inhibitors were normalized to vehicle control.
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bioRxiv - Genomics 2022Quote: ... Cells were harvested 72 hours post-transfection using the Passive lysis buffer (provided with the Dual Luciferase Reporter Assay system (Promega)) ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with the methanol TEAB buffer and then digested overnight at 37°C using 1ug Trypsin (Promega). Digested peptides were eluted and dried in a vacuum centrifuge before LC-MS analysis.
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated at 37°C for 72 h after which they were lysed for 20 min on a shaker platform at RT by addition of Reporter Lysis Buffer (Promega). Luciferase signal was determined from cell lysates by adding the Bright-Glo Luciferase buffer (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Cell Biology 2022Quote: ... The trapped proteins were washed four times with the methanol TEAB buffer and then digested using 1 µg Trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... negative control and input samples for 30 min at 37°C with shaking at 800 rpm with 0.05 U/µl of RQ1 RNase-Free DNase in 1X RQ1 RNase-Free DNase Reaction Buffer (Promega, # M6101). RNA was eluted from HisPur Cobalt Resin by incubating samples in a final volume of 100 µl of PBS with 0.4 mg/ml of Proteinase K (Macherey-Nagel ...
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bioRxiv - Microbiology 2020Quote: ... the cells were lysed with lysis buffer and luciferase activity was immediately measured by dual-glo luciferase assay system (Promega). The inhibition rate was calculated as (sera signals – blank control signals)/ (virus signals – blank control signals ...
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bioRxiv - Molecular Biology 2020Quote: ... After 48hr the cells were washed with PBS and lysed in a sonication bath in 150 ul of 1x Passive Lysis Buffer (Promega) supplemented with Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... used for FISH-Flow analysis or lysed in passive lysis buffer to measure luciferase activity using Luciferase Assay System (Promega). Relative light units were normalized to protein content determined by Bradford assay (Bio Rad).
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bioRxiv - Microbiology 2020Quote: ... cells were mock treated or treated with 10 unit/mL of uIFN for 6 hrs and collected in 1× passive lysis buffer (Promega). Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... cells were lysed in 60 µl of 1x passive lysis buffer of the Dual-Luciferase Reporter Assay System (Promega, E1980) and directly assayed or frozen at −20°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 5 times with wash buffer and the remaining bound cells were detected with the CellTiterGlo kit (Promega) as per the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Luciferase activity was measured after 48 hours (with or without drug treatment) using Reporter Lysis Buffer kit (Promega, Madison, WI) per manufacturer’s instructions and a Synergy H4 plate reader.
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bioRxiv - Microbiology 2022Quote: ... Twenty-five microliters of the substrate diluted at 1/20 in buffer (NanoGlo live assay, Promega, see the manufacturer’s instructions) was added extemporaneously ...
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bioRxiv - Microbiology 2022Quote: ... CrPV infection was performed at MOI 20 for 6 hours and then cells were lysed in passive lysis buffer (Promega) and luciferase activities measured by a dual-luciferase assay (Promega).