Labshake search
Citations for Promega :
2951 - 3000 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
-
bioRxiv - Cell Biology 2024Quote: ... The solution was diluted with 0.1 M ammonium bicarbonate buffer to lower the urea concentration to 1.5 M and digested with trypsin (Promega, Madison, WI, # V5111) at a 1:100 ratio (protease to protein (wt/wt) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed after 48 hours with Passive Lysis buffer and luciferase signal was measured on a Tecan SPARK 10M reader using the Dual-Luciferase Reporter Assay System (Promega, #E1910). Firefly luciferase signal was normalized to Renilla luciferase activity ...
-
bioRxiv - Neuroscience 2024Quote: ... cell pellets were washed with 1× PBS and resuspended in either RNA lysis buffer (mirVana kit) containing 0.5 U/μL RNAsin Plus (Promega; Madison, WI) for RNA isolation or 1X radioimmunoprecipitation assay (RIPA ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were homogenised at 2,000 rpm for 5 min using the MoBio PowerLyzer24 in a MoBio bead tube containing 0.1 mm diameter zirconia/silica beads and 750 µL of TLA buffer (Promega, WI, USA). Supernatant was recovered via centrifugation at 10,000 g for 30 s ...
-
bioRxiv - Microbiology 2024Quote: ... the protein pellets were resuspended in 100 µL 0.6M urea in 100mM TEAB buffer and 4 µg trypsin (Promega, Cat#V511B) was added for digestion at 1:25 enzyme:protein at 500 rpm at 37°C overnight ...
-
bioRxiv - Microbiology 2024Quote: ... 500 IU/ml of human interferon-gamma (IFN-γ) for the gamma activated sequence reporter for 18h and lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and assayed for firefly and Renilla luciferase activities using the Dual luciferase reporter assay system (Promega E1960 ...
-
bioRxiv - Biochemistry 2024Quote: Cells were harvested and resuspended in ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors (Promega and Sigma Aldrich, respectively). Lysates were centrifuged at 16,200 x g for 20 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were then pelleted and disrupted by glass bead agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega #E1910). Extracts were clarified by centrifugation ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatants were discarded and nuclei pellets were washed once with 4 mL Nuclei EZ Lysis Buffer and once with 4 mL of Nuclei Suspension Buffer (NSB) (PBS w/o Mg and Ca; 0.01% BSA; Biolabs ref B9000S; 40 U/mL RNAsin; Promega ref N251B). Final pellets were resuspended in 0.5 mL NSB containing DAPI (1 µL at 1 mg/mL ...
-
bioRxiv - Bioengineering 2024Quote: Single-molecule optical tweezer experiments were performed by incubating 51 nM restriction digested SHARP amplicon and 1 nM modified DNA unzipping handles 38 in 1x rapid ligation buffer (Promega #C6711) with T4 DNA Ligase (NEB #M0202L ...
-
bioRxiv - Cancer Biology 2024Quote: ... Medium was replaced 12 h after transfection and 48 h after transfection cells were lysed with 50 μL 1x passive lysis buffer diluted in water (Promega, E194A). Cells were frozen at -70°C for 30 min ...
-
bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The cDNA libraries were then prepared from the depleted RNA samples obtained during the previous step using the GoScript™ Reverse transcriptase (Promega) to synthesize the first strand cDNA and the TruSeq®Stranded mRNA Library Prep kit (Illumina ...
-
bioRxiv - Physiology 2020Quote: ... and single-stranded cDNA was synthesized from each sample (2 μg) with M-MLV reverse transcriptase (#0000113467, Promega Corporation, Fitchburg, WI) and RNase inhibitor (40 U ...
-
bioRxiv - Genetics 2021Quote: ... of G1 and G2 mosquitoes fluorescently expressing the actin5c::eCFP construct was extracted as individual samples using the Wizard® Genomic DNA Purification Kit (Promega). Regions of gDNA were then amplified according to the three primer pairs as indicated in Fig ...
-
bioRxiv - Bioengineering 2021Quote: ... Samples were diluted 10X in 50 mM ammonium bicarbonate after which 4 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) was added before overnight incubation at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA per sample was reverse transcribed to cDNA in a 20 μl reaction using M-MLV reverse transcriptase (Promega, USA) and random primers (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega), ligated and subsequently cloned into E ...
-
bioRxiv - Microbiology 2020Quote: ... The duplicate amplification products of each sample were pooled and purified with the SV Wizard PCR Purification kit (Promega, Madison, WI) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: DNA extraction was also done for all the samples using the Wizard® Genomic DNA Purification Kit according to the manufacturer’s protocol (Cat#A1120, Promega USA).The eluted DNA was quantified by QuantusTM Fluorometer® (Cat# E6150 Promega Technologies ...
-
bioRxiv - Cancer Biology 2022Quote: ... qPCRs were carried out by loading the same volume of DNA per sample and using GoTaq® qPCR Master Mix (Promega). Details of human-specific primers used are available in supplementary table S3.
-
bioRxiv - Microbiology 2021Quote: ... as described above was digested with chymotrypsin or trypsin/lysC using the Accumap™ low pH sample preparation with urea under nonreducing conditions (Promega). The free cysteines in the Mpro preparations (100 µg ...
-
bioRxiv - Plant Biology 2021Quote: ... and 100 μl sample volumes were assayed in triplicate for ATP content using the BacTiter-Glo bioluminescence kit (Promega, Mannheim, Germany). After 5 min incubation in the dark to allow for the decay of plate autofluorescence ...
-
bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
-
bioRxiv - Cell Biology 2021Quote: ... and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi) and digested overnight with 1 μg sequencing grade modified trypsin (Promega, V5111) at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
-
bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al ...
-
bioRxiv - Microbiology 2022Quote: ... Bacterial counts were obtained by comparing the signals from the tested samples with a standard curve prepared by serial dilutions of a standard sample purified using the WizardSV Gel and PCR Clean-Up System Kit (Promega, USA) and subsequently cloned into the pGEM-T vector (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... EPOP and Ser5-RNAPII and quality control of all immunoprecipitated DNA samples were tested by qPCR using GoTaq® qPCR Master Mix (Promega) with a QuantStudio 6 Flex Real-time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... DNA was extracted from samples using Maxwell® 16 automated system (Maxwell® 16 Cell DNA kits, Promega, Madison, WI, USA). Extracted DNA samples were quantified using NanoDropTM 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
-
bioRxiv - Cell Biology 2021Quote: ... Sample volume were equalized to 50 uL (5% of the samples were used as pulldown control for Westen blots analysis) and digested by LysC (Wako, #129-02541) and Trypsin (Promega, # V5111), and acidified using 2% TCA (Sigma ...
-
bioRxiv - Plant Biology 2023Quote: ... The firefly luminescence (LUC) from samples was immediately recorded for 5.0 sec on a GloMax 20/20 luminometer (Promega, Madison, USA) followed by 10-sec interval ...
-
bioRxiv - Genomics 2022Quote: ... The supernatant was discarded and approximately 0.2 mL of the sample was incubated with DNases (Promega and Epicentre, Madison, WI, USA), RNase A (Ambion ...
-
bioRxiv - Immunology 2022Quote: ... The samples were subsequently quenched with 10 mM DTT with overnight digestion at 37°C using Trypsin gold (Promega, WI, USA). The digestion was terminated and the surfactant was cleaved by treating the samples with 200 mM HCl (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 ng of cDNA of each sample was used for the qPCR reaction (duplicate/sample) with ENG or α-tubulin specific primers (Supp. Table 1) and the GoTaq qPCR Master mix (Promega), in presence of CXR reference dye ...
-
bioRxiv - Neuroscience 2023Quote: ... equal amount of RNA (2 μg) from each sample was subjected to cDNA synthesis using reverse transcriptase Kit (Promega Corporation, USA) following standard procedures ...
-
bioRxiv - Immunology 2023Quote: Culture supernatants from the different BMDMs samples were analyzed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega# G1780). Total cell lysate of non-treated (i.e. ...
-
bioRxiv - Genetics 2023Quote: ... The activity of the firefly and renilla for each sample were measured with a Promega GloMax® 96 Microplate Luminometer (Promega). Per well ...
-
bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated from the 102 human FFPE colorectal tissue samples using the Maxwell® RSC Blood DNA Kit (Promega) on a Maxwell® 16 MDx (Promega) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... After PCR pre-amplified cDNA were clean-up using 22% home-made PEG beads at a ratio of 0.7 beads:1 sample before concentration quantification using QuantiFluor® dsDNA System (Promega, #E2671). cDNA was normalised and diluted to 100pg µl-1.
-
bioRxiv - Physiology 2024Quote: Total RNA was extracted from powdered whole heart tissue samples using the SV Total RNA isolation kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions or from hiPSC-cardiomyocyte extracts with the TRIzolTM reagent (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: DNA was extracted from 200 mg of each fecal sample via bead beating and column purification using a Maxwell 16 MDx Instrument (Promega, USA), as previously described (Sikder ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
-
bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were generated by the S-Trap Method using S-Strap: Rapid Universal MS Sample Prep (Co2-mini,Protifi) and Trypsin Gold (Promega, V5280). Samples were then vacuum dried and resuspended in 50 μL of 1% formic acid (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2024Quote: ... Rosette leaf samples were ground in liquid nitrogen and total RNA was extracted using the SV total RNA isolation system (Promega, www.promega.com). After quantification with a Nanodrop system (www.nanodrop.com) ...