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Citations for Promega :
2801 - 2850 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The transfection was terminated 24 h later by removing the media and lysing the cells using 25 µl 1x Passive Lysis Buffer (Promega). For eIF1/eIF5 knockdown experiments ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Sorted nuclei were collected in 5-ml tubes containing 300-500 μl of collection buffer (PBS + 5% BSA) and RNasin Plus RNase inhibitor (Promega). Sorted nuclei were collected by centrifuging at 500g for 10 min at 4°C and processed for downstream analyses (RNA-seq ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of the culture was mixed with 10 µL with the NanoLuc Reaction Buffer of the Nano-Glo Luciferase Assay System (Promega). Luciferase activities were measured every 2 minutes with a Tecan Spark microtiter plate ...
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bioRxiv - Microbiology 2024Quote: ... 25 µL of NanoLuciferase substrate-lysis buffer mixture was added to 100 µL of faecal lysate (GloMax plate reader, Promega). Positive threshold for NanoLuciferase signal is RLU >1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Dissected tissue was homogenized in lysis buffer (10% Triton X-100, 0.1 M DTT, 40 U/μL RNasin (Promega, N2115), protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and subsequently lysed with 5X passive lysis buffer supplied with the luciferase assay kit (Promega). Following a brief vortex ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies were diluted in the same buffer and added to the cells ON at 4°C (mouse anti-HiBiT (Promega) catalog number CS2006A01 clone 30E5 ...
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bioRxiv - Systems Biology 2024Quote: ... followed by three washes with 100% acetonitrile before incubation with digestion buffer (50 mM TEAB, containing Sequencing grade Trypsin (Promega) at 1:25 enzyme-to-protein for 4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Rinsed beads were resuspended in 15 µL of 50 mM HEPES buffer (pH 8) supplemented with trypsin/LysC mix (Promega) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein : enzyme by weight, Promega V5280) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were lysed in 200 µL of CHAPS lysis buffer containing 200 units/mL of RNase inhibitor (Promega, N2511) on ice for 30 minutes and then centrifuged at 12000 × g for 20 minutes at 40C to collect the supernatant ...
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in 15 mL Ma lysis buffer (50 mM KxHyPO4 pH 7, 10 mM MgCl2) with 20 units of RQ1 DNase (Promega) and a Pierce protease inhibitor mini tablet (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were lysed in 200 µL of CHAPS lysis buffer containing 200 units/mL of RNase inhibitor (Promega, N2511) on ice for 30 min and then centrifuged at 12000 × g for 20 min at 4°C to collect the supernatant ...
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bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Both firefly and renilla luciferase activities in each of the same samples were measured by the Dual-Luciferase reporter assay system (Promega). ...
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bioRxiv - Neuroscience 2020Quote: ... corresponding samples (PSD-95 and MPP2) were incubated overnight at 37°C with 50 ng trypsin (sequencing grade modified, Promega) in 25 µl of 50 mM ammonium bicarbonate in the presence of heavy water (Campro Scientific GmbH ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: Genomic RNA was extracted from small intestine and large intestine samples using the SV Total RNA Isolation kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... We also included a mock community sample consisting of equal numbers of nine plasmids (pGEM®-T Easy vector; Promega) containing eight 16S rRNA gene sequences from honeybee gut symbionts and one from E ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 hours incubation with Wnt3A CM the cells were harvested in passive lysis buffer and both Firefly and Renilla luciferase activity was measured in the same sample using the Dual-Luciferase Reporter Assay System (Promega). The luminescence was quantified on a GloMax-Multi microplate reader (Promega ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from nasopharyngeal swab samples using Maxwell® 16 Viral Total Nucleic Acid Purification Kit (Promega, Wisconsin-USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 2μg RNA of each sample and oligo (dT)18 primers by M-MLV reverse transcriptase (Promega, 28025013). Three independent biological samples were collected and analyzed ...
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bioRxiv - Plant Biology 2021Quote: ... the still not size-separated single protein band per sample was cut, reduced (50 mM DTT), alkylated (55 mm CAA, chloroacetamid) and digested overnight with trypsin (trypsin-gold, Promega).
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted nine-fold with 50 mM ammonium bicarbonate and digested overnight with trypsin (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 75:1.
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the concentrated sample was removed from the ultra-centrifugal filter and a 1:50 ratio of mass spectrometry grade Trypsin Gold (Promega) added to the sample which was incubated with shaking for > 12 hours at 37 °C in a thermal heat block ...
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bioRxiv - Immunology 2020Quote: ... Genomic DNA from T0 and T9 (IgA+, IgA- and unsorted) samples was extracted using the Wizard Genomic DNA Purification kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 20μl of each sample was read on Fluo Star Optima plate reader with injection of 50 μl of Luciferase Assay Reagent (Promega).
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bioRxiv - Plant Biology 2021Quote: ... Residual genomic DNA was then removed from each sample with 10 U of RNAse-free DNaseI (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... For the RNA sequencing samples the isopropanol phase of TRIzol extraction was transferred to Reliaprep tissue Miniprep kit (Promega, USA) columns to ensure high quality RNA samples were used ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of bacterial culture was pelleted at 15,000 x g for 15 min at 4 °C then samples were prepared according to the directions for the Wizard HMW DNA Extraction kit for Gram-negative Bacteria (Promega). The University of Michigan Microbiome Core quantified the genomic DNA samples ...
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bioRxiv - Microbiology 2020Quote: The total genomic DNA (gDNA) content of each milk sample was extracted by an automated DNA extraction platform (Promega, UK) using previously published protocols6 along with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and activity of both luciferases was measured in 10 μL of the sample with the Dual Luciferase Reporter Assay system (Promega) on a Modulus Single Tube Reader (Turner Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... All samples were digested overnight at 37 °C using a Trypsin/Lys-C Mix (mass spec grade, Promega, Madison, WI) (enzyme/substrate of 1:100 w/w ...
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bioRxiv - Microbiology 2020Quote: ... DNA contaminations in the RNA samples were removed by DNase treatment according to the instructions provided by the manufacturer (RQ1 RNase free DNase, Promega). cDNA was synthesized from 2µg of DNA free RNA using MMLV reverse transcriptase and random primers (Promega) ...
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bioRxiv - Immunology 2021Quote: ... luminescence was measured at 18 hours post-treatment with untreated samples included as a control (GloMax Discovery, Promega, Madison, WI). Data was normalized to the untreated control and presented as the average (N=3 ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total RNA from two groups of samples were extracted using an SV total RNA isolation system with a DNase purification step (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were digested either with trypsin in a trypsin/protein ratio of 1/50 (w/w) (Promega Cat. No. V5111) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Urinary proteins (100 μg of each sample) were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Plant Biology 2020Quote: ... The luciferase assay was performed by thoroughly mixing 10 μl of a sample with 50 μl of the luciferin substrate (Luciferase Assay System, Promega) and measuring the luminescence intensity after 10 and 20 seconds using a luminometer (TD-20/20 Luminometer ...
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bioRxiv - Cancer Biology 2020Quote: ... digestion was continued by incubation of the sample at 37°C for 6 hours with trypsin (Promega, Catalog Number V5113) at an enzyme to substrate ratio of 1:75 ...
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bioRxiv - Molecular Biology 2023Quote: Protein samples were treated with Endoprotease Lys-C (Fujifilm Wako chemicals, Osaka, Japan) and Trypsin (Trypsin Gold Mass Spec Grade, Promega). Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies) ...
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and both the firefly and Renilla luciferase activities were measured from the same sample using the Dual-Luciferase Reporter Assay System (Promega) according to manufacturer instructions on a NOVOstar microplate reader (BMG Labtech) ...
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bioRxiv - Physiology 2023Quote: ... for 45 minutes as per the protocol described elsewhere(Villanueva et al, 2020) These samples were then subjected to trypsin (cat no V5111 sequencing grade, Promega) digestion in an enzyme to substrate ratio of 1:20 (trypsin ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were processed using the SP3 protocol (Hughes et al.2014) and on-bead digested with trypsin (sequencing grade, Promega), which was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...