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Citations for Promega :
3001 - 3050 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... The optical density of the standards and samples was recorded at 450 nm wavelength using a microplate reader (Promega, GloMax 9301). All samples were run in triplicates.
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bioRxiv - Molecular Biology 2022Quote: ... samples were diluted 8 times with 0.1M ammonium bicarbonate and sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) was added ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of RNA per sample was reverse transcribed to cDNA in a 20 µl reaction using M-MLV reverse transcriptase (Promega, USA) and random primers (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Biochemistry 2023Quote: ... Excess iodoacetamide was quenched by addition of 5 mM DTT and samples were then digested by trypsin digestion (Sequencing Grade Modified Trypsin, Promega Corp) at 37°C for 6 hours in a Thermomixer with shaking (1000 rpm ...
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 μL of samples was added into white-walled 96-well plates and equal volume of ready-to-use Tluc substrate (Promega; E1501) was injected to each well ...
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bioRxiv - Physiology 2024Quote: ... Protein (50 μg per sample) was digested overnight at 37°C with LysC (Wako Chemicals) at 1:50 (w/w) ratio and trypsin (Promega, V5113) at 1:100 (w/w ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2 µl of the lysed samples were added to 25 µl PCR reactions with GoTaq Green mastermix (Promega, Cat. No. M7123). After amplification ...
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bioRxiv - Cell Biology 2024Quote: ... the Firefly and Renilla Luciferase activity was measured in triplicate for each sample using the Dual-Luciferase Reporter Assay System (Promega, Australia). Luciferase activity was expressed as a ratio of Renilla to Firefly activity (Van Etten et al. ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were incubated for one hour at 47°C with 2 µg of sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB ...
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bioRxiv - Molecular Biology 2024Quote: ... as previously described by our lab.21 RNA was eluted and lysate samples were spiked with 5 ng of Luciferase control RNA (Promega L4561) as a housekeeping control for total RNA isolation efficiency in qPCR assessment of HuR-bound target genes.
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bioRxiv - Immunology 2024Quote: DNA has been extracted from patient blood cell samples for HLA Class I typing using the DNA Isolation Kit (Wizard HMW DNA Extraction Kit from Promega, GER). DNA was used for Luminex-based high-definition LABType rSSO typing (One Lambda ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of protein was diluted 5 times with 50 mM ABC and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested 24 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 10 times with 50 mM ABC (no Guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: Tryptic fragments were further digested with chymotrypsin by dissolving the samples in 20 mM ammonium carbohydrate containing 0.5 ng/µL chymotrypsin (Roche, Basel, Switzerland; Promega, WI, USA) and incubated at 37°C for 5 h ...
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bioRxiv - Cell Biology 2024Quote: The insulin secreted from islets ex vivo and plasma insulin from blood samples a were measured using Lumit Insulin Immunoassay kit (Promega, CS3037A05) as per the manufacturer’s instructions and measured using a Flexstation-3.
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bioRxiv - Cancer Biology 2024Quote: DNA has been extracted from patient blood cell samples for HLA Class I typing using the DNA Isolation Kit (Wizard HMW DNA Extraction Kit from Promega, GER). DNA was used for Luminex-based high definition LABType rSSO typing (One Lambda ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were treated with mass spectrometry grade trypsin (4.0 mL, 2.0 mg, Sequencing grade modified trypsin, Porcine, Promega, Cat. No. V511A) and incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... All the samples were collected and stored at -20 °C for assay of glucagon with either Lumit Glucagon kit (Promega; W8020) or glucagon ELISA kit (MSD ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml ionomycin for 6h then lysed in passive lysis buffer commercially provided in the dual luciferase reporter assay kit (Promega, no. E1910). Assay was conducted according to manufacturer’s instructions and luminescence measurements were done using CLARIOstar (BMG ...
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bioRxiv - Developmental Biology 2022Quote: ... β-galactosidase assays were performed with the same cell extract using the β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer (Promega, #E2000). The luciferase activity was normalized against the lacZ activity of each culture ...
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bioRxiv - Microbiology 2020Quote: ... the cells were harvested and lysed in reporter lysis buffer, and luciferase activity (relative luminescence unit, RLU) was measured using luciferase assay reagents and a luminescence counter (Promega, Madison, WI). Percent inhibition of serum antibodies compared to the level of the virus control subtracted from that of the cell control was calculated ...
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bioRxiv - Microbiology 2020Quote: ... 5% CO2 after transfections and after 48 hours were lysed using passive lysis buffer and assayed for Fluc and Rluc activity using a dual luciferase assay kit (Promega, Madison, USA) (13).
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bioRxiv - Cell Biology 2021Quote: ... Bait proteins were eluted with 25µl of elution buffer (2M Urea, 0.67M thiourea in 50mM Ammonium bicarbonate pH 8.0) containing LysC/Trypsin (Promega, Madison, WI, cat# V5071) to a final concentration of 5µg/ml followed by two elution with 50µl of elution buffer without LysC/Trypsin ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μl of Reporter Lysis 5X Buffer was added to each well to complete a single freeze-thaw cycle (Promega, Cat. # E4030). Then ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested two days post-transfection and lysed using the Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
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bioRxiv - Microbiology 2021Quote: Cells were washed with 1X Dulbecco’s PBS and harvested with 100 μL of 1X passive lysis buffer diluted in ddH2O (Promega, Madison WI, USA). Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... a standard 12.5 μl translation reaction was mixed with 50 μl 1x Renilla-Glo substrate in Renilla-Glo assay buffer (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
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bioRxiv - Immunology 2021Quote: ... A reverse transcription cocktail was subsequently prepared in the following manner (per reaction): 5µl of M-MLV 5X reverse transcriptase buffer (Promega, Catalogue no.-M531A), 5µl of 10 mM dNTP mix (Bioline ...
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bioRxiv - Synthetic Biology 2023Quote: ... the transfected cells were lysed with 1x Ex Luciferase Assay Buffer using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). Immediately after ...
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bioRxiv - Synthetic Biology 2023Quote: ... lysed with 1X Passive Lysis Buffer then analysed using the Dual-Luciferase Assay kit on a GloMax multi+ plate reader (Promega, Southampton, UK).
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bioRxiv - Molecular Biology 2022Quote: ... Pelleted cells were disrupted by glass beads agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Extracts were clarified by centrifugation ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Genomics 2024Quote: ... A frozen piece of each liver (∼ 50 mg) was homogenized in 500 μl ice cold 1x Passive lysis buffer (Promega, cat. #E1910) using a rubber-tipped micro pestle fitting the bottom of a 1.7 ml centrifuge tube ...
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bioRxiv - Bioengineering 2024Quote: ... 50 µL of the cell and 1xPBS mixture were transferred into a 96-well plate bottom white plate (Costar Cat#3922) and lysis buffer from Nano-Glo Luciferase Assay Kit (Promega Cat#N1120) was added directly to the plates following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies were taken from the agar plates and added to 5 µL Green GoTaq Reaction Mix Buffer (Promega, Madison, WI, USA), 1.25 units GoTaq DNA Polymerase (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... In a pre-warmed solution of Reconstitution Buffer (20mM HEPES, 250mM KCl, 2mM TCEP, pH 7.5) containing 40 U/mL RNAsin (Cat#N2111, Promega, Madison, WI, USA), each Sm protein sub-complex was combined to a final concentration of 8 µM and incubated for 5 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... clarified by centrifugation (500 x g, 5 min, at 15°C) and mixed 1:1 with 2× passive lysis buffer (Promega cat #E1941). Cell monolayers were washed in PBS and lysed in 1× passive lysis buffer ...