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Citations for Promega :
2701 - 2750 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Immunology 2022Quote: ... followed by a PBS wash and lysis buffer to measure the luciferase activity in cell lysates using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Pathology 2020Quote: The cellular ATP levels were quantified in 100 μL ATP assay buffer or from 20-30 mg wound skin harvested in 200 μL assay buffer and lysed with the bead shocker following the manufacturer’s instructions (CellTiter-Glo luminescent ATP assay kit, Promega, Madison). The protein content from identical treated cells or tissues were determined by BCA Protein Assay kit for normalization (Young ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Microbiology 2020Quote: ... 4 µl of the lysates were mixed with 20 µl of Renilla Luciferase Assay Buffer and 1/200 of substrate from the Renilla Luciferase assay system (Promega) and measured immediately in a luminometer for 2 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... before re-suspending in 50 μl flow buffer containing anti-Nluc polyclonal primary antibody raised in rabbit (Kindly gifted by Promega) at 1:100 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blots to assess the abundance of the proteins were performed on the same volumes of lysates obtained from the luciferase measurements using the passive lysis buffer supplied with the Dual-Luciferase Reporter Assay System (Promega). Antibodies used for western blots were previously described (Grozdanov et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Trpc4 and Trpc5 mRNAs was performed on 3 µl of cDNA from single cells in a 30 µl reaction volume containing 1X GoTaq Flexi buffer (Promega), 2 mM MgCl2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested in passive lysis buffer and luciferase assay was performed using Dual-Luciferase Assay System (Catalogue # E1960, Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed with passive lysis buffer 48 hours after infection and reporter activity was then measured with a Nano-Glo luciferase assay system (Promega). For IFN-I based experiments increasing amounts of IFN-I were added 16 hours before infection and for TRIM25 overexpression experiments cells were reverse transfected with either TRIM25 or YFP as well as TIM1 24 hours before infection.
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bioRxiv - Evolutionary Biology 2021Quote: ... Linear replicon DNA (500 ng) was added to transcription reactions at a final volume of 100 μl containing the following: Transcription Optimised Buffer (Promega), 10 mM DTT (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were then washed twice as described above and submitted to overnight proteolysis in ammonium bicarbonate buffer containing 1 μg of trypsin (Promega) per sample at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Transcription reactions were carried out for 20 minutes at 37 °C in a reaction volume of 250 μL containing 20 μg of plasmid template in 1x transcription buffer (Promega; 40 mM Tris-HCl pH 7.9 / 6 mM MgCl2 / 10 mM spermidine / 50 mM NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was suspended in buffer P1/RNAse A and lysed by adding 1/20 volume of 10% SDS (Promega). After incubating for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and then developed in the dark with fresh developing buffer supplemented with 225 ug/ml nitro blue tetrazolium (NBT) (Promega) and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays require washing the harvested cells 3 times in PBS prior to lysis in passive lysis buffer (Promega, E1941). NLuc activity was assayed using the Nano Luciferase Assay Kit (Promega ...
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bioRxiv - Immunology 2020Quote: ... Media was removed and cells were washed in PBS before re-suspension in 75 μL 1X Passive Lysis Buffer (PLB; Dual-Luciferase Reporter Kit, Promega). Cells were incubated in PLB for 15 min at RT and further homogenised by pipetting ...
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bioRxiv - Immunology 2020Quote: ... The plate reader was programmed to inject 40 μl of Luciferase Assay Substrate re-suspended in Luciferase Assay Buffer II and 35 μl of 1X Stop&Glo Reagent (Dual-Luciferase Reporter Kit, Promega) per well ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Developmental Biology 2020Quote: ... lysed in 100µl of passive lysis buffer and standard dual luciferase assays were performed on the cell lysates by using Dual-Luciferase Reporter Assay reagents (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed in the plate with 250 μl of 1x Passive Lysis Buffer and luciferase activity was determined using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... 120,000 nuclei were sorted using an SH800 sorter (Sony) into 87.5 μl of collection buffer (1U/µl RNAsin (Promega, N2515), 5% fatty acid-free BSA (Proliant ...
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once with PBS and lysed for at least 1 h with 40 μl 1x passive lysis buffer (Promega) at RT ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... 120 μg of fragmented total RNA was added to the antibody-bound beads in 250 μl IP buffer supplemented with RNasin Plus RNase inhibitor (PRN2615, Promega), and the mixture was incubated at 4 °C for 2 h ...
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed after exactly 24 hours of stimulation in 1X Passive Lysis Buffer (100 μl per well (Promega, #E1941)) ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
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bioRxiv - Microbiology 2023Quote: ... After removal of supernatant cells were resuspended in 100 ul of freshly prepared 1X Renilla Luciferase Assay Lysis buffer (Promega). Samples were analyzed with the Renilla Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Samples were harvested at specified timepoints with 1x passive lysis buffer before measurement of NLuc activity using the Nano-glo luciferase assay system (Promega) with the GloMax-96 luminometer (Promega).
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The media mixture was removed from each well and the cells were lysed with a room tempered 1xGlo buffer (Promega) for 5 min at 25°C while shaking at 550 rpm ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 6 or 24 hours as indicated in the respective figure legends before lysis using the HiBiT lytic assay buffer (Promega) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were harvested 24 hours post transfection using 1X passive lysis buffer and the dual luciferase assay was performed according to instructions from manufacturer (Promega). Luciferase signals were read using a BioTek Synergy H1 plate reader (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Powdered plant material (150 mg) was aliquoted and extracted with 1.25 mL hexadecyltrimethylammonium bromide (CTAB) buffer (MC1411, Promega, Madison, WI) containing proteinase K (ref 25530049 ...
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...