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Citations for Promega :
2601 - 2650 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and several sample types were evaluated for ADCC and ADCP functionalities using the corresponding Reporter Bioassay kit (Promega Corporation). ADCC and ADCP induction was expressed as induction ratio (IR) ...
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bioRxiv - Immunology 2021Quote: The RNA samples isolated from the lungs of immunized and challenged mice at 5DPI were treated with DNase (Promega), and then converted into cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from clinical samples (300μl) via a Maxwell 48 device using the Maxwell RSC Viral TNA kit (Promega) with a viral inactivation step using Proteinase K ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from filter paper punches using the manufacturer’s protocol for Promega Maxwell DNA IQ Casework Sample kit (Promega) or Qiagen DNA Blood kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... Samples were diluted in 100 mM ammonium bicarbonate and incubated overnight with 0.5 μg/μl sequencing-grade trypsin (Promega) at 37°C ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Genetics 2022Quote: ... Samples were then diluted 10fold with 20 mM Tris and trypsin (Trypsin Gold, Promega, #V5280, 1:200 w/w) and digested overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Complementary DNA was prepared from total RNA extracted from indicated samples using the ImProm-II Reverse Transcription System (Promega). qRT-PCR analysis was performed using a master mix with ROX reference dye (Invitrogen) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then transferred to white 96-well plates and 50 μL of Bright-Glo Luciferase Assay Reagent (Promega) was added and mixed by manual orbital agitation ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein were digested overnight at 37°C in 2 or 1.5 M urea (for BB22OP and RIMD 221063 samples, respectively) and 25 mM ammonium bicarbonate with modified trypsin (Promega), in a 1:50 (M/M ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Assessment of SL drug cytotoxicity in 3D samples was performed using a CellTiter 3D Cell Viability Assay (Promega, #G9681) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were resuspended in ammonium bicarbonate and digested overnight with 0.2 µg of sequencing grade modified trypsin (Promega) at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA samples (from three independent plants in all cases) were subjected to reverse transcription with the AMV enzyme (Promega) and random hexanucleotides as primers ...
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from FFPE tumor patient samples using the ReliaPrep™ FFPE Total RNA Miniprep System (Promega). RNA Integrity was determined using the Fragment Analyzer ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested for 2 hr at 25°C with constant agitation with a Trypsin/LysC mix (V5071, Promega), and overnight at 25°C with proteomics-grade porcine trypsin (T6567 ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation47 with sequencing grade modified porcine trypsin (Cat. No. V5111, Promega). Nano-liquid chromatography tandem mass spectrometry (Nano-LC/MS/MS ...
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bioRxiv - Microbiology 2023Quote: The LDH levels in supernatant samples were measured using the CytoTox 96 Non-23 Radioactive Cytotoxicity Assay (Promega, G1780) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 1μg samples of purified RNA were reverse transcribed into cDNA with M-MLV Reverse Transcriptase (Promega, Madison, WI, USA). 25ng samples of cDNA were then used in SYBR Green real-time PCR reactions on a Step-One real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Thereafter the samples were processed according to the Manufacturer’s Protocol for ReliaPrep RNA Tissue Miniprep System (Promega, Fitchburg, USA). PBMCs were isolated using histopaque density gradient centrifugation (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Cell Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation (Wiśniewski, Zougman, Nagaraj, & Mann, 2009) with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 µm resin (Waters ...
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bioRxiv - Synthetic Biology 2023Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... The platform also permits a high-throughput automated genomic DNA isolation from 48 samples in 40 minutes (Promega, 2020) making this system compatible with larger sample sets.
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bioRxiv - Biochemistry 2023Quote: ... each sample was digested overnight at 37 °C with one or more of the following enzymes separately: trypsin (Promega), Lys-C (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were initially digested for 1 hour at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB (pH 7 ...
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bioRxiv - Genomics 2023Quote: ... Total RNA from ED and TE samples was isolated using the ReliaPrepTM RNA Cell Miniprep System (Promega, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cDNAs were synthesized from 500 ng of total RNA for each sample using M-MLV reverse transcriptase (Promega, M1701) and (dT)25 according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: RNA from lyophilized cerebrum and brainstem samples was extracted using the Maxwell® RSC simplyRNA Tissue Kit/Instrument (Promega) with initial homogenization on a TissueLyser LT (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from FFPE samples (Discovery Cohort) using Maxwell 16 LEV RNA FFPE Kit (Promega, Madrid, Spain), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence (relative luminesce units, RLU) and Absorbance (OD595) of sample triplicates supplemented with NanoGlo Live Cell Assay System (Promega) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Genetics 2024Quote: ... Each cDNA sample was amplified in quadruplicate in a 25-μL mixture containing 0.75 U GoTaq DNA polymerase (Promega), 200 μM dNTPs ...
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bioRxiv - Plant Biology 2024Quote: ... The alkylation was stopped by the addition of 1 µl of 200 mM cysteine and the samples digested overnight with 1 µl of 0.5 µg/μl trypsin (MS Gold, Promega). The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... 50 μl of 50 mM TEABC pH 8.0 was added to samples before trypsin digestion was carried out using modified sequence-grade trypsin (Promega) with 1:50 trypsin:protein ratio at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Samples were prepared according to the directions from the Wizard HMW DNA Extraction kit for Gram-negative Bacteria (Promega).56 The genomic DNA was rehydrated in DNA Rehydration Solution and Illumina sequenced (SeqCoast) ...
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bioRxiv - Immunology 2024Quote: ... Samples were digested overnight with LysC (Wako, 1:100 enzyme: protein ratio) and trypsin (Promega, 1:50 enzyme:protein ratio). Samples were desalted with a 96-well mini 20MG PROTO 300 C18 plate (HNS S18V ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then diluted to 1 M urea and subjected to digestion with trypsin (Promega, 1:50 w/w) overnight at 37⁰C ...
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bioRxiv - Systems Biology 2024Quote: ... The digested samples were combined with 5 μL of 6 x 5 LC-MS/MS Peptide Reference mix (Promega) and stored at-20℃ until analysis.
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bioRxiv - Systems Biology 2024Quote: ... pH 8.5). Samples were prepared using the SP3 protocol (Hughes et al. 2014, 2019) and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and digested on-bead using a filter-aided sample preparation (Wiśniewski et al., 2009) with sequencing grade-modified porcine trypsin (Promega). Tryptic peptides were then separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Cell Biology 2024Quote: ... and digested on-bead using a filter-aided sample preparation (Wiśniewski et al., 2009) with sequencing grade-modified porcine trypsin (Promega). Tryptic peptides were trapped and eluted on 3.5um CSH C18 resin (Waters ...
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bioRxiv - Biochemistry 2021Quote: ... The residual magnetic beads were then incubated for two hours at 4°C and for one hour at 16°C in 83 microlitres of buffer A200 with 20 Units RNasin (Promega), 12mM DTT and 16 micrograms of TEV-Protease ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were lysed in the lysis buffer that was included in the Dual-Luciferase® Reporter Assay System (Promega, E1910). To the cell lysate ...
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bioRxiv - Developmental Biology 2021Quote: ... Limb fragments were immediately transferred in 1.5 ml LowBind tubes with 500 ul of ice-cold lysis buffer (Reliaprep RNA Tissue MiniPrep System, Promega #Z6111), vortexed ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated for 6 h at 37°C before being harvested in 1× Passive Lysis Buffer and subjected to a Dual-Luciferase Reporter Assay System as per manufacturer’s instructions (Promega, E1980) on a GloMax-Multi plate reader (Promega) ...