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Citations for Promega :
2851 - 2900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Digestion was started by adding to the cartridges 20µg of Trypsin (Promega) in 125 μl of 50 mM TEAB ...
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bioRxiv - Cell Biology 2024Quote: ... Fugene-HD (Promega) was used according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was gel-purified and cloned into the pGL3-control plasmid (Promega) at the XbaI and SalI sites ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA samples were additionally pre-treated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell viability was assessed by Cell Titer-Glo Luminescent Cell Viability (CTG) assay (Promega) and measured by Varioscan Lux plate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... Positive cells were selected using antibiotic Zeocin and Luciferase reporter assay was performed using Dual- Glo luciferase assay kits (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 72 hr MTS assay was performed following the manufacturer’s instructions (Promega).
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bioRxiv - Cell Biology 2024Quote: ... and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) was performed using the M-MLV Reverse Transcriptase (Promega). Total RNAs and genomic DNA from lavage fluids and mouse tissue homogenate were extracted using AllPrep® DNA/RNA Micro Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... first with 1 µg r-LysC Mass Spec Grade (Promega) for 4 h at 30°C and then samples were diluted to 1.8 M urea with 100 mM Tris HCl pH 8.5 and 1 µg Sequencing Grade Modified Trypsin was added for the second digestion overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the gel was transferred to a PVDF membrane and western blotting was performed using an anti-Halo primary antibody (Promega, #G9211) and a goat anti-mouse secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2024Quote: ... the Halo-CCSer2650–850 was mixed with a 10-fold excess of Halo-TMR (Promega) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the Lis1-bound beads were mixed with SNAP-AlexaFluor 647 (Promega) for 10 minutes at room temperature after the lysis buffer wash ...
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bioRxiv - Cell Biology 2024Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Cell Biology 2024Quote: ... and SYK(A353T)-NL) were kindly provided by Promega. NL orientations used in the respective assays are indicated ...
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bioRxiv - Cell Biology 2024Quote: ... and normalised to double-stranded DNA (dsDNA) content to account for differences in islet mass (IEQ) using the QuantiFluor dsDNA system (Promega, USA) as per manufacturing instructions.
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bioRxiv - Cell Biology 2024Quote: ... Both assays were done according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Cell Biology 2024Quote: ... 25 uL of Magne HaloTag Beads (Promega) were washed twice with 1 mL GF150 supplemented with 0.1%NP40 ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid constructs were transfected into HEK293 cells using Viafect reagent (Promega), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... then diluted to 1M urea and digested with trypsin (Promega, 1:50 w/w) overnight at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and luciferase activities were measured by using the dual luciferase reporter assay (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin (mass spectrometry grade; Promega, V5280) was used at a ratio of 1:30 (sample:enzyme ...
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bioRxiv - Cancer Biology 2024Quote: ... 70 ng of a firefly luciferase reporter construct containing XPA sequences and 30 ng of the Renilla luciferase control (pRL-TK; Promega) were mixed with 0.2 µl of Jetprime transfection reagent in 10 µl of Jetprime transfection buffer (Polyplus ...
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bioRxiv - Cancer Biology 2024Quote: IC50 was measured by quantifying the percentage of live cells using a CellTiter-Fluor™ Cell Viability Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the viability of each well was assessed using the CellTiter-Glo reagent (Promega).
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bioRxiv - Cancer Biology 2024Quote: Apoptosis was assessed by Caspase-Glo 3/7 assay according to manufacturer’s instructions (Promega). A total of 10,000 cells/well were grown in 24-well clear plates and treated with 5.5 µg (in vivo concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured using CellTiter Glo 2.0 (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... and a human mixed genomic DNA (PROMEGA) (0 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% v/v of RQ1 RNase/DNase Stop Solution (Promega) and 10% v/v Oligo(dT ...
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bioRxiv - Neuroscience 2024Quote: ... and subsequently transfected using the FuGENE HD Transfection Reagent (Promega, Madison, US) 28–48 h before measurement.
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from stem cells using Wizard Genomic DNA Purification Kit (Promega). APOE genotyping was carried out using the TaqMan SNP Genotyping Assay and TaqMan Genotyping Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Cells received 1:1 maturation medium and CellTiter-Glo® Reagent (Promega, Madison, WI, USA). Plates were shaken at 480 rpm for 2 min followed by 10 min incubation at room temperature in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... containing 4% D-luciferin (Promega). The luciferase substrate permeated the cells for 120 min at 37 ℃ ...
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bioRxiv - Neuroscience 2024Quote: ... MS-grade trypsin and LysC were purchased from Promega, Madison ...
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bioRxiv - Neuroscience 2024Quote: ... and trypsin (Promega sequencing grade #V5111), as described by91 ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat. M170A, 1.25µM RT 5X buffer, Promega Cat. M531A, 0.5mg/mL Oligo (dT) 18 Primer ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR (qRT-PCR) was performed in triplicates with the GoTaq qPCR Master Mix (Promega) on a LightCycler 480 Instrument (Roche Diagnostics) ...
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bioRxiv - Neuroscience 2024Quote: ... further digestion was performed by the addition of trypsin (cat. # V5111, Promega), (enzyme-to-substrate ratio ...
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bioRxiv - Neuroscience 2024Quote: ... Cell viability was assessed 72 hours post-treatment using the CellTiter-Glo Assay (cat. # G7572, Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Luminescence was measured using the Dual Glo Luciferase Kit (Promega) following the manufacturer’s protocol in an ELISA plate reader (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were digested with trypsin/Lys-C Mix (Promega Corporation, USA), and 10 μg of each sample was used for proteomic analysis ...
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bioRxiv - Neuroscience 2024Quote: ... with 1 μg of Lys-C (Fujifilm-Wako) at 37 °C for 4 h followed by digestion with 0.2 μg of trypsin (Promega) and mixed gently at 37 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Proteolytic digestion was performed by consecutive digestion with LysC (0.1 µg; 4 h) and trypsin (0.1 µg; 16 h) at room temperature (Promega, Germany).
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bioRxiv - Neuroscience 2024Quote: ... and ribonuclease (RNasin Recombinant, Promega) at concentrations of 1:1000 (sucrose buffer ...
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bioRxiv - Cancer Biology 2024Quote: Human H1-0 promoter sequence (nucleotides −351 to +161 from TSS) was PCR-amplified from REH cell genomic DNA and inserted into Firefly luciferase vector pGL4.22 (Promega, #E6771) at KpnI and HindIII restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using M-MLV reverse transcriptase (Promega, #M1701) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and the transcriptional activity was quantified using the dual luciferase assay system from Promega.
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bioRxiv - Cancer Biology 2024Quote: ... Plates were incubated for 72 hours and viability was determined by CellTiter-Glo Luminescent viability assay (Promega) using a Tecan SPARK 10M reader ...
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bioRxiv - Biophysics 2024Quote: ... The dried fragments were then digested with trypsin by adding 12.5 ng/μL trypsin (Promega) in 50 mM NH4HCO3 for 16 hours at 37°C ...