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Citations for Promega :
2551 - 2600 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... A few millimeters from the ventral lobe of the caudal fin were collected and immediately stored in -70°C or stored in 80µl of lysis buffer (Promega). 50mg of fin samples were homogenized in Z742682 BeadBug™ 6 microtube homogenizer (Merck KGaA ...
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bioRxiv - Cancer Biology 2024Quote: ... About 200 mg tissue after ground to powder by liquid nitrogen was transferred to a preheated (65℃) 2.0 ml tube with appropriate amount of CTAB lysis buffer (#MC1411, Promega) and was mixed by vortex ...
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bioRxiv - Microbiology 2024Quote: ... and cells were lysed by adding 100 µl of 1X-passive lysis buffer (Dual-Luciferase reporter assay system, Promega), and freeze-thawing ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media was centrifuged at 1000g for 10 min and supernatant was either suspended in Passive Lysis buffer (Promega) (total hTau ...
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bioRxiv - Genomics 2024Quote: In total 120k nuclei were sorted using an SH800 sorter (Sony) into 87.5μl of collection buffer (1 U/µl RNAsin (Promega, N2515), 5% fatty acid-free BSA (Proliant ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The dissected tissues were directly collected in LB+TGA lysis buffer from Reliaprep RNA Tissue Miniprep System (Promega, USA), and homogenized using a Biomasher Standard homogenizer (Takara Bio Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of Nano-Glo Luciferase Assay Reagent (1:50 mixture of Nano-Glo Luciferase assay substrate:Nano-Glo Luciferase assay buffer (Promega) was added to each well containing the diluted samples ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were trypsinized on beads in 100 μL of digestion buffer (1 M urea, 50 mM Tris-HCl, pH 7.5, and 5 μg/mL trypsin (V5111, Promega)) ...
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bioRxiv - Systems Biology 2024Quote: ... The rinsed beads were resuspended in 115 μL of 50 mM HEPES buffer (pH 8.0) supplemented with trypsin/LysC (Promega) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Immunology 2024Quote: ... the RNA/cDNA double-stranded hybrid was then added to 1.5 μl of Blue Buffer (Enzymatics), 1.1 μl of dUTP mix (10 mM dATP, dCTP, dGTP and 20 mM dUTP) (Promega), 0.2 μl of Rnase H (5 U/μl) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then lysed by Glo lysis buffer and measured by Bright-Glo Luciferase Assay System according to the manufacturer’s protocol (Promega). Enhanced green fluorescent protein (EGFP ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 µL of HiBit buffer (LgBit 1:200, Promega, N112A; Furimazine 1:100 Promega, N113A in nuclease-free water) were added ...
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bioRxiv - Microbiology 2024Quote: ... the cells were infected with SFV-FFLuc (MOI=0.1) and lysed at 48 hpi using 1X Passive Lysis Buffer (PLB; Promega). FFLuc levels were measured using a Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2024Quote: ... Transposition was performed directly on nuclei using 25µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina and Digitonin #G9441 from Promega). After pelleting the nuclei by centrifuging at 500g for 10 min ...
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bioRxiv - Physiology 2024Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... The RNA bound to the magnetic beads was then washed with Wash Buffer (WSB) on a magnetic rack (Promega). RNA was eluted with Elution Buffer (EB ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein: enzyme by weight, Promega) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Digestion buffer (50 mM TEAB pH 8.0) containing sequencing-grade modified trypsin (1/25, w/w; Promega, Madison, Wisconsin) was added to the S-trap column and incubate for 2h at 47 °C ...
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bioRxiv - Biochemistry 2024Quote: ... was added to 0.5 mL 1% bovine serum albumin (BSA) in IP/lysis buffer (Pierce, 87788) and incubated with MagneHis beads (Promega, 32 µL suspension ...
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bioRxiv - Cell Biology 2024Quote: ... This reaction was then ligated into pGEM t-easy vector with T4 ligase and T4 ligation buffer (Promega #A1360) so that individual alleles could be sequenced ...
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bioRxiv - Cancer Biology 2024Quote: ... the peptides in each sample were eluted with 60μL of ice-cold Elution Buffer I (0.012g Urea, 50μL of 1M Tris-HCl pH7.5) and 5μg/mL Trypsin (Promega, Seq Grade Modified) for 30 mins at RT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the aqueous phase was collected in EtOH and RNA lysis buffer from the SV Total RNA Isolation kit (Promega), and the manual instruction was followed ...
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bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
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bioRxiv - Cancer Biology 2020Quote: ... BXPC-3 and CAPAN-1 pancreatic cancer cells treated with UR214-9 was measured using the Cell Titre96R Aqueous One Solution Cell Proliferation Assay (Promega Corp., catalog number: G3580) following the procedure published earlier ...
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bioRxiv - Microbiology 2021Quote: ... and samples were incubated at room temperature for two hours before stopping the reaction with 30 μL of the DNase stop solution (Promega Corp. Madison, WI, USA). The eight DNase-treated samples underwent DNA extraction at this point ...
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bioRxiv - Molecular Biology 2024Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium (MTS) assay (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega, Madison, WI, USA). Ramos cells were seeded in 96-well plates at density of 105 cells/well and incubated for 48 h prior to experimental treatments ...
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bioRxiv - Microbiology 2022Quote: ... DNA from the saliva samples (n=67, one missing) was extracted using the ReliaPrep Blood gDNA Miniprep (Promega, USA). For microbiome profiling ...
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bioRxiv - Molecular Biology 2020Quote: RNA from chicken samples was isolated by Reliaprep™ RNA Tissue Miniprep System according to manufacturer instructions (Promega, USA), followed by cDNA synthesis using GoScript Reverse transcription mix (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were mixed by shaking the plates after which luminescence was recorded with a GloMax Explorer Model GM3510 (Promega) 10 min after adding the reagent.
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... Samples were then digested with 2.5 micrograms of sequencing grade trypsin (Trypsin Gold, Mass spectrometry grade, Promega, Madison, WI) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The 4% PFA was removed from the samples with 3 washes of PBSTriton (0.1% Triton-X-100 (Promega: H5141) in 1x Phosphate Buffered Saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... the samples were lysed and assayed using the SteadyGlo reagent kit and a GloMax luminometer (Promega, Maddison, WI, USA). To measure HCVcc replication ...
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bioRxiv - Microbiology 2020Quote: ... Dried reduced/alkylated samples were then rehydrated with 20 ng/µl trypsin in 100 mM NH4HCO3 (Promega, Madison WI) on ice for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and the sample was digested at 37 °C with sequencing-grade trypsin (Promega; 1:50 protease:protein w/w ratio) or at 25°C with Glu-C (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of guanidium hydrochloride was reduced to 0.5 M by dilution with 100 mM ammonium bicarbonate and the samples were digested with 0.5 μg sequencing grade trypsin (Promega) over-night at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Cell Biology 2021Quote: ... then samples were digested overnight at 37°C on a rotator with modified trypsin (1:20 trypsin:sample ratio) (Promega). Peptides were desalted using SepPak C18 columns (Waters ...
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bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
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bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Proteolytic digestion was performed overnight at 37°C using 0.5 μg Trypsin/Lys-C Mix Mass Spectrometry Grade for each sample (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: Illumina P5 and P7-barcoded adaptors were added by PCR on gDNA samples according to the GoTaq protocol (Promega). PCR amplicons were gel purified and sequenced on a HiSeq4000 (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: gDNA samples for library preparation were extracted from overnight LB cultures using the Wizard Genomic DNA Purification kit (Promega) as described above ...
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bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (Promega) at 1:10,000 and peroxidase was detected using the Pierce chemiluminescence system (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were diluted 10-fold with 50 mM HEPES pH 8 and incubated with sequence-grade modified Trypsin (Promega) at 1/50 trypsin/protein ratio for 16 h at 37 °C ...