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Citations for Promega :
2351 - 2400 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: Protoplasts were flash frozen in liquid nitrogen and lysed by adding 30 μl of Passive Lysis Buffer (Promega). NanoLuc activity was then measured using the Nano‐Glo® Luciferase Assay (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were collected in 500μl of cell lysis buffer and processed using RealiaPrep RNA Cell Miniprep System (Promega), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were treated with 3 µM PP242 for 2 h and lysed with Passive Lysis Buffer (Promega, E194A). Luminescence was detected with a Dual- Luciferase Reporter Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega). The samples were incubated for 30min at 22°C prior to magnetic capture of the protein-RNA complex ...
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bioRxiv - Genetics 2020Quote: ... cells were washed with 1× phosphate-buffered saline (PBS) then lysed with 1× passive lysis buffer (E194A, Promega). Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega) ...
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... and were then suspended per gram of wet cell pellet in 1.5 millilitres of buffer A200 with 0.04U/millilitre RNasin (Promega), 1 mM phenylmethylsulfonyl fluoride and 2 mM benzamidine ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed with PBS and lysed with 100μL Passive Lysis Buffer (Dual luciferase Reporter Assay System, Promega). Firefly luciferase (FL ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was carried out in 10 μl of Buffer M containing 0.4 U/µL of RNasin (Promega) in the presence of 0.02 μM cspA mRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... EVs designated as exosomes were separated by centrifugation at 100,000xg for 75 min and were resuspended in 200μl of 1X passive lysis buffer (Promega) for isolation of exosomal proteins and RNA ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: In ovo tumors were collected and lysed in the buffers for the GSH/GSSG-Glo assay kit (Promega) according to the manufacturer’s instructions and normalized for protein concentration (Pierce BCA protein assay kit ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Microbiology 2023Quote: Cell supernatants were collected and stored in LDH storage buffer + Triton 10% as per the manufacturer’s instructions (Promega). Supernatants were inactivated for 2 hours at room temperature prior to usage outside the BSL-3 ...
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bioRxiv - Neuroscience 2022Quote: ... 0.4 μl of each normalized sample was mixed with 1.2 μl of Tn5 Tagmentation mix (0.64 μl TAPS-PEG buffer (PEG 8000, V3011, PROMEGA, and TAPS-NaOH pH8.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent; Promega). The average luminescence of the background cultures was subtracted to the average luminescence of each sample.
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bioRxiv - Developmental Biology 2023Quote: ... modified hypotonic lysis buffer with a 10-fold higher concentration of Cycloheximide and Recombinant RNasin Ribonuclease Inhibitor (Promega) was used instead of RNase OUT ...
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bioRxiv - Cell Biology 2024Quote: 20×106 cells were collected in cold PBS by scraping and lysed in RIP buffer (251mM Tris-HCl pH 7.4, 1501mM KCl, 5mM EDTA, 0.5% Nonidet P-40, 201U1ml−1 RNAsin (Promega), 0,51mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2023Quote: ... The digestion was performed in a 50 mM TEAB buffer with 0.2 μg of Lys-C (Wako Chemicals) and 0.4 μg of trypsin (Promega). Trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Synthetic Biology 2022Quote: ... where 40 µl LARII buffer was added to measure Fluc signal in a GloMax 96 Microplate Luminometer (Promega) with a 2-s delay and a 10-s measurement ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL wash buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were then resuspended to an OD600 of 50.0 in lysis buffer (1x FastBreak cell lysis reagent (Promega), 50 μg/mL lysozyme ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... and IRF3-5D luciferase assays cells were lysed 20 hours post transfection in 1x passive lysis buffer (Promega). Luciferase activity was measured with the dual luciferase assay system (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Microbiology 2024Quote: ... The cells were then lysed 7 h later in 20 µl of Renilla luciferase assay lysis buffer (Promega), and luciferase activity was quantified using a Tristar LB 941 luminometer (Berthold Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was recovered with 100ul of elution buffer and measured with QuantiFluor® ONE dsDNA System (Promega, # E4871). DNA was stored at −80°C until use.
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with 1X PBS and lysed using lysis buffer in the Luciferase assay kit (Promega). For each virus ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed and lysed in 1x passive lysis buffer (Dual-Luciferase Reporter Assay, Promega, cat. no. E1980), and luminescence signals were captured by GloMax 96 microplate luminometer (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Ten nanograms of template DNA was added to 5 µL Green GoTaq Reaction Mix Buffer (Promega, Madison, WI), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 mL of barcode buffer (dPBS with 0.5% BSA and 5 μg/mL Herring DNA (Promega)) and then stored at 4°C prior to loading with the probe pool ...
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bioRxiv - Molecular Biology 2024Quote: The frozen tissues (∼25 mg) were homogenized in 400 µL of 1x reporter lysis buffer (#E1501 kit, Promega), and the lysates underwent 3 freeze thaw cycles (-80°C to 37°C) ...
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bioRxiv - Microbiology 2024Quote: ... Assays in 12-well plates (all others) were lysed with 200 µL of Glo Lysis Buffer (Promega, #E2661) for 5 min (RT) ...
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bioRxiv - Immunology 2024Quote: ... the cells were lysed in 40 μl (for a 96-well plate) of 1X Glo Lysis buffer (Promega) for 10 min at room temperature ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were carried out in 10 µL-volumes containing 1x GoTaq® reaction buffer (Promega, Southampton, UK), 0.1 mM dNTPs ...
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bioRxiv - Cell Biology 2022Quote: Proliferation assays were performed in 96 well plates (2 × 103 NMCCs/well) and assayed at 48 h using the CellTiter96® AQueous One Solution Cell Proliferation Assay kit (MTS; Promega, Madison, WI) (38) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: MTS growth assays were performed on adherent cells by plating 1000-5000 cells per well in a 96-well plate and assaying using CellTiter 96® AQueous One Solution (Promega, Madison, WI) and incubating for 2 hours and reading at 490nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The evaluation of the cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the antiviral activity assay and measured by using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates and then maintained in the presence of vehicle or increasing doses of GSK591 for six days before the addition of 20 μL CellTiter 96 AQueous One Solution per well (Promega Corporation, Madison, WI). Plates were incubated for 2 hours before absorbance at OD490 was measured with a Synergy H4 Hybrid microplate reader (Bio Tek ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability at 72-h incubation was measured by MTS cell proliferation colorimetric assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay system, Promega, G3582, Italy). Cell numbers at 0 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times and the reaction was developed with 100 µL ready-to-use TMB solution (Promega, Madison, WI, USA) at RT for 20 min in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the neutralization assay and evaluated using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2024Quote: ... the viability of HAEs was assessed by a MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega, Madison, WI, USA) by addition of 20 µl of MTS solution with 180 µl of Opti-MEM on the apical pole of HAEs ...