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Citations for Promega :
2451 - 2500 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then diluted with 25 mM ABC and digested with sequencing-grade modified porcine trypsin (Promega) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2024Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega) using Clariostar plate reader ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Neuroscience 2024Quote: ... 500 ng of RNA samples were reverse transcribed using the M-MLV Reverse Transcriptase (Cat# M1701, Promega, USA) following the reverse transcription kit instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were further diluted to 2 M urea with 100 mM TEAB and trypsin (Trypsin Glod, Promega) was added at a ratio of 1:50 and the samples were incubated at 37°C over night ...
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bioRxiv - Plant Biology 2024Quote: ... At least 3 independent technical experiments were performed from each RNA sample using SYBR Green Master Mix (Promega) with Chromo4 Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... 100µl of cell lysate from each sample were used with the Dual-Luciferase Reporter Assay System (Promega #E1910) to measure relative β-catenin activity.
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA samples were converted to cDNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase enzyme (Promega), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... All samples were then digested overnight at 37°C with using 500 ng sequencing grade modified trypsin (Promega).
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bioRxiv - Bioengineering 2024Quote: ... All RNA samples first underwent a DNAse step (1 µL DNAse/12.2 µLRNA, RQ1 RNAse-Free DNAse, Promega), then reverse transcription using a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... One microgram (µg) of DNase treated RNA sample was used to synthesize cDNA using GoScript Reverse Transcriptase (Promega) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were stored at −80°C according to the protocol of ReliaPrepTM RNA Tissue Miniprep System (Promega). cDNA was synthesized from 400 ng of total RNA using PrimeScript RT Master Mix (Perfect Real Time ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested at varying timepoints by resuspending the cells in 50-100 μL of 1X Passive Lysis Buffer (Promega). Inductions were staggered so that all timepoints could be harvested simultaneously ...
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bioRxiv - Genetics 2021Quote: ... dCTP-Cy3 (GE) was incorporated along with standards dNTPs for visualization purposes using random priming (Labeling 5X Buffer Promega). For the fixation ...
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bioRxiv - Molecular Biology 2021Quote: ... the RNA/oligonucleotide mixture was diluted to 30 µl final with a reaction mix (1X RNase H reaction buffer, 65 µM DTT, 0.5U/µl RNasin (Promega), 50 U RNase H (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: Muscles were pulverized in liquid nitrogen using a chilled mortar and pestle (Plattner’s) and re-suspended in passive lysis buffer (Promega). Luciferase results were obtained by reading 5 µL of sample with 50 µL luciferase assay reagents in a Glomax Luminometer according to manufacturer’s specifications.
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bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were incubated at either 15°C or 37°C for 10 min in Buffer I containing 0.4 U/µL of RNasin (Promega). Following renaturation ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Immunology 2021Quote: ... Beads were washed with urea buffer and trypsinized on-bead overnight at room temperature with 20 μg trypsin (Promega). The next day ...
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bioRxiv - Systems Biology 2020Quote: ... The solution was diluted to below 1M Urea using 200mM bicarbonate buffer and an aliquot of approximately 25μg protein were digested using sequencing grade Trypsin (V511A, Promega) at 37°C over-night (Trypsin to protein ratio of approx ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: Medium from the basolateral layer of the tissue culture inserts was removed 24- and 96-hours post-infection and diluted in LDH Storage Buffer as per the manufacturer’s instructions (LDH-Glo Cytotoxicity Assay, Promega). Samples (5 μl ...
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bioRxiv - Cell Biology 2021Quote: ... collected at 2000 x g followed by resuspension in 0.5 ml of storage buffer (PBS containing 4% BSA and RNasin (Promega) – 0.2 U/ul ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were lysed by a 15-min incubation at room temperature with passive lysis buffer (E1941, Promega, Madison, USA). Cell lysates (30 μL ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were harvested by washing once with PBS wash followed by addition of 22µl of Passive Lysis Buffer (Promega) under shaking for 15min ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were washed three times with TBS buffer containing 0.1% Tween 20 and finally the membranes were developed with BCIP/NBT color development substrate (Promega) to enable visualization of protein bands (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... First the crosslinked RNA was dephosphorylated using 0.1U/μL FastAP (Thermofischer) in Reaction buffer containing 1U/μL RNAsin (Promega) for 45min at 37°C and stopped by washing once with Denaturing buffer and three times with Reaction buffer supplemented with 0.5% NP-40 ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 1 μg/mL freshly prepared coelenterazine h (Promega). Cells were plated in white-walled ...
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bioRxiv - Microbiology 2022Quote: ... The multiplex PCR was performed in a 12.5 μl volume containing 2.5 μL of 5x GoTaq® Reaction Buffer (Promega), 0.25 μL of dNTP (10 mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... before the addition of digestion buffer (50 mm Tris) containing 0.8 μg Lys-C (Wako, 129–02541) and 0.8 μg Trypsin-gold (Promega, V5280). Enzymatic digestions proceeded under agitation (400 rpm ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Microbiology 2022Quote: ... and cells were rinsed in 1X PBS and lysed by the addition of 40 µl passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
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bioRxiv - Developmental Biology 2022Quote: ... Tails were dissected from the injected embryos at 23-24 hpf and lysed with passive lysis buffer (E1941, Promega) for subsequent Dual-Luciferase assay following the manufacturer’s instructions (E1910 ...
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bioRxiv - Microbiology 2022Quote: ... HEV) or at 33°C (HRV) until harvested by washing in PBS and lysis in Passive Lysis Buffer (Promega). Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium) ...
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bioRxiv - Immunology 2021Quote: ... plates were washed 5 times with wash buffer (PBS with 1% v/v bovine serum albumin (Capricorn Scientific) and 0.05% v/v Tween-20 (Promega)) ...
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bioRxiv - Molecular Biology 2020Quote: ... After 48hr cells were washed with PBS and lysed in a sonication bath in 1x Passive Lysis Buffer (Promega) supplemented with Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... The pellets were then subjected to one freeze-thaw cycle before being resuspended in Passive Lysis Buffer (Promega; E1910). One scoop of 0.15 mm zirconium oxide beads (Next Advance ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed using passive lysis buffer and luciferase activities were measured using Luciferase® reporter assay system (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of template DNA was added to 5 μl Green GoTaq Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Evolutionary Biology 2022Quote: ... diluted in a luciferase assay buffer (PBS, 0.1% BSA) and mixed with furimazine (0.25% Nano-Glo® Substrate, Promega). The samples were distributed in duplicates in 96 half-well white polystyrene microtiter plates ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells (1.2 x 106 cells/well, 6-well format) after 24 hpi using passive lysis buffer (Promega) based on the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and the cells were lysed with 30 µl/well of 1× cell lysis buffer (Promega) for 15 min ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions with 50 ng plasmid template were performed in 50 µl volume containing 10x DreamTaq buffer and 2.5 units of DreamTaq polymerase (Promega). Standard PCR conditions were 94 °C for 5 min ...
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bioRxiv - Plant Biology 2021Quote: ... Purified nucleus were recovered in 300 μl of Nuclei Lysis Buffer (50 mM Tris-HCl pH8; 0.1% SDS; 10 mM EDTA; 100 μM PMSF; RNasin PROMEGA) and 5 cycles (30 s ON – 30 s OFF ...
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bioRxiv - Cancer Biology 2020Quote: ... Organoids from 2 wells of a 24 wells plate were collected in 500μl of cell lysis buffer and processed using RealiaPrep RNA Cell Miniprep System (Promega), according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of the total RNA was incubated in oxidization buffer (50 mM NaIO4 and 100 mM NaOAc pH 5.2) supplemented with RNasin (Promega) for 30 min at 25°C to oxidize the nonacylated tRNA 3’ end ...