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Citations for Promega :
2251 - 2300 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were incubated in freshly made stain solution (5mM potassium ferricyanide + 5mM potassium ferrocyanide + 1mg/mL X-gal (Promega cat no. V3941) in wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured for 1 day in 96-well plates and treated with small molecules for 3 consecutive days prior to incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...
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bioRxiv - Cancer Biology 2023Quote: ... The beads were washed using four cycles of resuspension and pulse centrifugation in Dynabeads wash solutions containing 0.05% Tween 20 (H5152, Promega, Madison, WI, USA), followed by three washes in 1X PBS (10010072 ...
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bioRxiv - Biophysics 2024Quote: ... The samples were dried again and rehydrated with 20 µl of trypsin solution with 0.01% ProteaseMAXTM surfactant comprised of 10 ng µl-1 Trypsin (Promega Corp., Madison, WI) in 25 mM NH3HCO3 / 0.01% w/v of ProteaseMAXTM from Promega Corp ...
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bioRxiv - Microbiology 2024Quote: THP-1 macrophage viability was determined by colorimetric method using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega; G35C) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h after the drug treatment using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Spain). PBS-treated cells were used as control ...
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bioRxiv - Systems Biology 2024Quote: ... plates were incubated for 10 days in 12L:12D conditions and treated with 50 µl of 1:50 of furimazine substrate solution (Promega, Southampton UK): 0.01% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell proliferation was analyzed after 48 hours culture using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The A2780 cell proliferation was analyzed 48h after cultured in CM using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell growth and viability were assessed using the MTS CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, Wisconsin, US) after 72 hours of treatments ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were developed with a commercial solution of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) according to the manufacturer (Promega, Madison, WI), in alkaline developing solution ...
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bioRxiv - Microbiology 2020Quote: ... The amount of total ATP in each sample was determined immediately using the BacTiter-Glo kit (Promega).
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Genetics 2021Quote: ... protein samples were digested overnight at 37°C with 125 ng modified porcine trypsin (Trypsin Gold, Promega). Peptides were extracted under acidic conditions and dried out using a speedvac concentrator ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was proteolyzed by adding 10 μl of 1.25 μg/μl sequencing grade modified trypsin (Promega) dissolved in 100 mM TEAB and incubated overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were then resuspended in 50 µL of 50 mM TEAB and digested with trypsin (Promega, #V1115) overnight at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... samples from homozygous HaloTag-TEV mice were incubated overnight with 5 μM HaloTag Oregon Green ligand (Promega) in Phosphate-buffered saline (PBS ...
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bioRxiv - Plant Biology 2020Quote: ... We synthesized cDNA using 2.0 μg of total RNA per sample and M-MLV Reverse Transcriptase (Promega) and poly-T primers (Sigma-Aldrich).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alternatively 20 μl samples were assayed using a Dual-Glo Luciferase Assay System using manufacturers protocol (Promega). Luminescence (RLU ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were diluted 4-fold prior to overnight digestion at 37 °C with trypsin (Trypsin Gold, Promega) at an enzyme to substrate ratio of 1:50 ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant from each sample containing precisely 10 mg of protein was digested with Trypsin Gold (Promega) at 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Microbiology 2020Quote: ... the samples were lysed and assayed using the SteadyGlo reagent kit and a GloMax luminometer (Promega, USA). A no-envelope pseudovirus was used to calculate the signal-to-noise ratio ...
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bioRxiv - Plant Biology 2020Quote: ... Samples of RNA were reverse transcribed using the GoScript™ reverse transcription system and oligo (dT20) (Promega). RT–qPCR was performed in a 20 μl reaction with the Fast Start Universe SYBR Green Master (ROX ...
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bioRxiv - Microbiology 2021Quote: ... The pooled samples were then concentrated using a Wizard SV Gel and PCR Clean-Up System (Promega). Quality control of the pooled and concentrated sample was done using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Biophysics 2020Quote: ... The samples were further digested overnight at 37 °C with the addition of sequencing grade chymotrypsin (Promega) to a final protease:protein (w:w ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Digestion was performed for all samples using the same procedure: 500 ng of Sequencing Grade Trypsin (Promega) were added for digestion carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with trypsin (trypsin to substrate ratio of 1:50, Promega, cat. no. V5111) and 1 mM CaCl2 for 16 h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... to remove genomic DNA from sample and protected against RNA degradation using RNasin® Ribonuclease Inhibitor (Promega). RNA concentration and quality were then measured using a NanoDrop™ 2000 spectrophotometer.
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bioRxiv - Immunology 2020Quote: ... Bacterial groups in cecal samples were measured by real-time PCR using GoTaq qPCR Master Mix (Promega) on Applied Biosystem 7900HT Fast or StepOne Plus™ Real-Time PCR Systems ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA from each sample was treated with RQ1 RNase-Free DNase (Promega Co.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were treated with Endoprotease Lys-C (Wako) and Trypsin (Trypsin Gold Mass Spec Grade; Promega). Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... Total genomic DNA of these two samples was obtained using Wizard Genomic DNA Purification Kit (Promega, Inc.) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... A 5µl aliquot of the sample was added to a 10µl ImProm-II reverse transcription reaction (Promega). The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was isolated from EDTA blood samples using the Maxwell RSC Whole Blood DNA kit (Promega).
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 6 h at 37°C with 3 μg Sequencing Grade Modified Trypsin (Promega). Digestion was terminated via addition of 20 μL trifluoroacetic acid (TFA) ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 h at 37°C (sample to enzyme ratio 1:20) and then with GluC (Promega) (1:40 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega; M-1302). Real time qPCR was preformed using a QuantiNova SYBR Green kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: Amplified cDNA samples were size selected using ProNex Size-Selective Purification System (Promega Corporation, Madison, WI, USA) as per the PacBio recommendation for standard length cDNA transcripts ...
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bioRxiv - Cancer Biology 2020Quote: ... after isolating DNA from mouse tissue samples using a tissue DNA extraction kit (Maxwell® 16, Promega), the purified DNA was incubated and hydrolyzed enzymatically (EpiQuik ...
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bioRxiv - Genetics 2022Quote: ... Samples were diluted 1:4 with 50mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... and samples were digested over night with Lys-C (Wako) followed by 4h digestion with Trypsin (Promega) at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were incubated overnight at 37°C with 0.01 μg/μL of trypsin (Promega, Madison, WI) and dissolved in 50 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2022Quote: ... Residual genomic DNA was removed by treating with 10 units/sample of RNAse-free RQ1 DNAse (Promega) for 1h at 37°C followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2022Quote: ... 500 μL of the sample were used for DNA extraction using the Maxwell Cell tissue kit (Promega) and subjected to Illumina sequencing ...