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Citations for Promega :
2301 - 2350 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Promoter fragments corresponding to wild type and mutant p53 target genes were amplified by standard PCR using DNA from healthy donors and subsequently cloned into the pGL4.2 basic vector (Promega Corporation, Madison, USA) for promoter-luciferase assays ...
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bioRxiv - Immunology 2023Quote: ... 4 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, #A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The identity of PCR products was verified by Sanger sequencing of amplicons cloned using the pGEM-T cloning system (Promega, Madison, WI) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Biophysics 2021Quote: ... The 2725-nt RNA molecules were synthesized by polymerase chain reaction (PCR) amplification of the plasmids followed by in vitro transcription using the T7 RNA polymerase (Promega, Fitchburg, WI, USA). The RNAs contain a 1206-nt upstream sequence ...
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bioRxiv - Cell Biology 2020Quote: ... Two microliters aliquots of the generated cDNA was amplified in 50 μl of PCR reaction mixture containing 2X GoTaq® green master mix (Promega, Madison, WI), 100 nM of each primer and ddH2O ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Cell Biology 2019Quote: An attR3/attR4 MultiSite Gateway cassette was PCR-amplified from pK7m34GW (Karimi et al., 2005) using GoTaq DNA Polymerase (Promega UK, Southampton, UK) with primers NdeI-R3 and NdeI-R4 (Table S2) ...
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bioRxiv - Molecular Biology 2019Quote: The putative LINC00662 binding regions within the ELK4 gene were amplified by PCR and cloned into downstream of pmirGLO dual-luciferase vector (Promega, Madison, WI, USA) to form the wide-type plasmid (ELK4-3’UTR-Wt ...
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bioRxiv - Neuroscience 2020Quote: ... 40ng of each cDNA preparation (a quantity calculated from the RNA concentration on the assumption that reverse transcription of RNA into cDNA was complete) was used to perform PCR using GoTaq® DNA polymerase (Promega, Madison, USA). Each 25μL PCR reaction contained 0.2mM of deoxyribonucleotide triphosphates (dNTPs) ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified PCR products were used as templates to synthesize dsRNA using the T7 RiboMAX Express RNAi System (Promega Corporation, Madison WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Capped cRNAs of the two Atlantic salmon PepT2 were synthesized by in vitro transcription using T7 RNA polymerase from cDNAs in pSPORT1 linearized with NotI and purified with Wizard SV Gel and PCR clean-up system (Promega Italia, Milan, Italy). The purified cRNAs were quantified by NanoDropTM 2000 Spectrophotomer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting PCR reaction was then cloned into pGEM T-Vector system and selected for using the XGal/IPTG system (Promega, Madison, WI, USA). Sanger sequencing was performed at the University of Pennsylvania Genomic Analysis Core with the SP6 promoter/primer ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified by agarose gel electrophoresis and extracted from the gel using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2019Quote: ... Supplementary Table 4) and DNA sequencing of the products following clean-up (Wizard SV Gel and PCR Clean-Up System, Promega, Southampton, Hampshire, UK) using dye terminator chemistry (Eurofins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Full-length coding sequences were cloned into the transcription vector pTB-207 and RACE-PCR product were cloned into pGEM-T (Promega, Madison, WI, USA). Eurofins Genomics (Luxembourg ...
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bioRxiv - Developmental Biology 2020Quote: ... All RACE-PCR products were purified using GeneJET Gel Purification Kit (Fermentas, Hanover, MD, USA) and ligated into pGEM-T vector (Promega, Madison, WI, USA) and transformed into competent JM-109 Escherichia coli cells (Promega ...
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bioRxiv - Microbiology 2019Quote: ... The primer pairs used in dsRNA synthesis are shown in Supplemental Table 1.1 μg PCR product was used as the template for dsRNA synthesis utilizing the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The dsRNA was isopropanol-precipitated ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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Highly Versatile, Non-Invasive Method for Collecting Buccal DNA from Free-Ranging Non-Human PrimatesbioRxiv - Genetics 2021Quote: ... 750 µL of each supernatant was processed using a commercially available DNA clean-up system (Wizard SV Gel and PCR Clean-Up System; Promega, Madison, WI, USA), and the DNA was finally eluted with 50 µL pure water ...
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bioRxiv - Molecular Biology 2022Quote: For high-resolution DNA electrophoresis resolving the nucleosome repeats genomic DNA was purified from chromatin samples using the Wizard SV Gel and PCR Clean-Up System (Promega, catalog no. A9281) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Biophysics 2022Quote: pHalo-H2B was generated by PCR amplification of the H2B coding region from an H2B-GFP template and cloned into a pFC14A backbone (Promega, Madison, WI, USA) to fuse the HaloTag to the C-terminus of H2B (56).
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was run on a 2% agarose gel using gel electrophoresis and the band was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA). Samples were submitted to Macrogen Europe (Amsterdam ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Developmental Biology 2020Quote: ... Digoxigenin-labelled riboprobes for the four stag genes were synthesized from PCR clones inserted into pGEM®-T Easy vectors (Promega, Madison, Wisconsin, USA) using T7/Sp6 RNA polymerase (Roche Diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... The product of the ITS gene amplification was cleaned and purified using the Wizard□SV Gel and PCR Clean-Up System (Promega, Madison, Wl, United States), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products of the expected size were eluted from the gel and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The PCR products were then cloned into a pCR2.1-TOPO vector using the Topo TA Cloning kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual genotyping at every generation was performed via PCR on genomic DNA extracted from larvae pseudopods (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI, USA) using gene-specific primers (forward ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2023Quote: ... using the described conditions for PCR in 25 µL using GoTaq® G2 DNA Polymerase and Master Mix (Promega Benelux BV, Leiden, the Netherlands), 10 µM of each primer ...
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bioRxiv - Plant Biology 2023Quote: ... The PHN-SpCas9-4×BsaI was then digested with BsaI and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The annealed oligos FaPDSF1/R1 and FaPDSF2/R2 were mixed and ligated to the purified pHN-SpCas9-4×BsaI to form the final pHN-SpCAS9-FaPDSsgRNAs construct.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...