Labshake search
Citations for Promega :
1951 - 2000 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... The reaction products served as templates for PCR with GoTaq ® G2 Hot Start Master Mix (Promega, USA), 300 nM forward primer ...
-
bioRxiv - Genomics 2022Quote: ... The PCR products that showed an obvious difference in HRM were cloned into pGEM-T easy vector (Promega) and sequenced by the Sanger method at the DNA Sequencing and Genotyping Facility at the University of Chicago Comprehensive Cancer Center.
-
bioRxiv - Microbiology 2022Quote: ... The GFP and mEOS2 tags were amplified by PCR from peGFP and pmEOS2 using Pfu Polymerase (M7741, Promega), dNTP mix (10297018 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Digested plasmids and gBlock DNA fragments were purified by Wizard SV Gel and PCR clean-up system (Promega) and transformed in Stbl3 competent cells ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products of attS were further cloned using the pGEM-T easy vector system (Promega, Madison, WI, USA) and DH5α competent cells to analyze the sequence variation of PCR products.
-
bioRxiv - Immunology 2022Quote: ... MCMV-DNA was quantified by real-time PCR using BIO-RAD CFX with GoTaq qPCR Master Mix (Promega) and primers specific for MCMV glycoprotein B (gB ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were inserted into the pGEM-T easy vector kit as described by the manufacturer (Promega, USA). Insert were then PCR-amplified and the resulting PCR products were sequenced.
-
bioRxiv - Molecular Biology 2023Quote: ... The resulting templates were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega) and transcribed using the Megascript T7 kit (Thermo Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... The fluorescently labeled PCR products were digested by using the 4-bp cutter TaqI (Promega, Southampton, United Kingdom) as described by the manufacturers ...
-
bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected by using SYBR Green I (GO-Taq PCR Master mix, Promega, Walldorf, Germany; A6002). Suitable reference genes were identified by using geNorm [47] ...
-
bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected using SYBR Green I (GO-Taq PCR Master mix, Cat: A6002; Promega, Walldorf, Germany). Sequences of the primers used in this study are listed in Supplementary Table S2 ...
-
bioRxiv - Neuroscience 2023Quote: ... The PCR product was subsequently confirmed using sanger sequencing and transcribed in vitro using T7 RNA polymerase (Promega) and Digoxygenin (DIG ...
-
bioRxiv - Cell Biology 2022Quote: ... The A-tailed PCR products were then cloned into the pGEM®-T Easy Vector Systems (Promega #A1360) followed by transformation ...
-
bioRxiv - Cancer Biology 2023Quote: ... Conserved Elements (CE) were PCR-amplified from GB genomic DNA using GoTaq® G2 DNA Polymerase (Promega, #M7845) (primer sequences listed in Supplementary Table S1 ...
-
MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
-
bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation, Madison, WI, USA), without fragment separation ...
-
bioRxiv - Microbiology 2023Quote: ... A PCR product matching the correct target size was gel purified and ligated into pGEM-T Easy (Promega) to yield plcl::Kn ...
-
bioRxiv - Immunology 2023Quote: ... the Renilla luciferase (Rluc) gene was PCR-amplified from phRL-TK (Promega, Madison, WI, USA, cat. no. E6241), and the eGFP gene was PCR-amplified from a codon-optimized synthetic gene fragment obtained from Bionics Inc ...
-
Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... quantitative real-time PCR was performed in Rotor Gene 6000 (Corbett Research) using GoTagR qPCR Master Mix (Promega) according to manufacturer’s instructions with the following primers:
-
bioRxiv - Plant Biology 2024Quote: Each 20 µl endpoint PCR reaction contained 10 µl of Gotaq Green Master Mix (2X) (Promega, Madison, WI), 1 µl (5 µM ...
-
bioRxiv - Genetics 2023Quote: ... PCR products were cleaned with ExonucleaseI (ExoI)(Fermentas; Pittsburgh, PA) and Shrimp Alkaline Phosphatase (SAP) (Promega; Madison, WI) incubated at 37°C for 30 minutes and 80°C for 15 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... each WT ZNF410 peak was amplified by PCR from genomic DNA and cloned into pGL4.24 vector (Promega, #E8421), digested by KpnI and XhoI ...
-
bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions and PCR amplification was performed using the GoTaq Flexi DNA polymerase kit (Promega). Organoid genotype was confirmed by Sanger sequencing ...
-
bioRxiv - Developmental Biology 2023Quote: ... The amplicon was purified prior to sequencing using a Wizard SV Gel and PCR Clean-Up System (Promega).
-
bioRxiv - Immunology 2024Quote: ... Each mutation locus was amplified in separate PCR reactions using GoTaq Green Master Mix (Promega Cat. No. M7123). PCR reactions were held at 95 °C for two minutes followed by 35 cycles of 45 seconds at 95°C for denaturation ...
-
bioRxiv - Microbiology 2024Quote: Correct integration of the homology template at the desired location was assessed by PCR (GoTaq DNA Polymerase, Promega) on extracted genomic DNA (Promega Wizard DNA Extraction ...
-
bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the final PCR product was gel-purified and subcloned into the pGEM-T Easy Vector (Promega, Guangzhou, China) for sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: ... a region of the COX1 gene was amplified by PCR using GoTaq® G2 Green Master Mix (Promega, Australia) and primer set LepF (5’-TTCAACCAATCATAAAGATATTGG-3’ ...
-
bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sequences of interest were amplified from the cDNA through PCR and ligated into the pGEM-T-Easy vector (Promega). Ligation products were cloned into JM109 cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... All the reactions were performed in a CFX-Connect real-time PCR detection system (Bio–Rad, www.bio-rad.com/) using GO Taq qPCR master mix (Promega, USA). Each experiment was repeated at least three times ...
-
bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed on a Bio-Rad CFX96 Real-Time PCR system with the GoTaq qPCR Master Mix (Promega). Relative gene expression levels were calculated using the ΔΔCt method with CPA as the reference gene.
-
bioRxiv - Microbiology 2019Quote: ... The resulting amplicons were pooled by sample and purified using the Wizard SV-Gel and PCR Cleanup System (Promega). Samples were sequenced by the UW-Madison Biotechnology Center on an Illumina Hi-Seq 2500 in rapid mode ...
-
CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR was performed with 16 ng of cDNA per reaction with GoTaq qPCR Master Mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
-
bioRxiv - Synthetic Biology 2019Quote: Two to three colonies of TOP10 transformed cells were selected to verify through PCR with GoTaq DNA Polymerase (Promega) if the plasmids they contained were the desired constructions ...
-
bioRxiv - Bioengineering 2019Quote: ... Linearized plasmid DNA was purified using the Wizard® SV Gel and PCR Clean-up Kit (Promega, Madison, WI) in preparation for transfection into Jurkat cells.
-
bioRxiv - Biochemistry 2019Quote: ... After confirmation of their amplicon size by 0.8% Tris-Acetate EDTA agarose gel electrophoresis and purification by Wizard SV Gel and PCR Clean-Up System (Promega), the sequences of their ORFs were confirmed using BigDye Terminator V3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
-
bioRxiv - Microbiology 2020Quote: Approximately 105 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert:vector and incubated overnight at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... and purified by gel extraction using the Wizard SV gel and PCR clean-up system (Promega, Fitchburg, WI, USA) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Presence or absence of transcript was detected by a regular PCR using GoTaq® G2 DNA Polymerase (Promega, M7841) from 100ng cDNA and visualized on an agarose gel.
-
bioRxiv - Cell Biology 2021Quote: The viral titers were determined by quantitative real-time PCR using GoTaq® qPCR Master Mix (Promega, Madison, WI) on a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... The multiplex PCR was performed in a 12.5 μl volume containing 2.5 μL of 5x GoTaq® Reaction Buffer (Promega), 0.25 μL of dNTP (10 mM) ...
-
bioRxiv - Microbiology 2022Quote: Each colony PCR consisted of a 25-μl reaction containing: 12.5 μl 2x GoTaq Green Master mix (Promega, UK), 0.5 μl of the reverse and forward primers (10 μM each) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega). All mixtures were made in 1x NEBuffer 3.1 on ice with final concentrations of 80nM Cas9 ...
-
bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...