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Citations for Promega :
1901 - 1950 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The digested product was gel-extracted using the Wizard SV Gel and PCR Clean-Up Kit (Promega). The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a CFX96 q–PCR System using SYBR Green qPCR master mix (Promega, USA). GAPDH was used as the internal control ...
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bioRxiv - Microbiology 2019Quote: Plasmid pGEMt was used for the ligation of purified PCR products according to manufacturer’s protocol (Promega A1360, USA). The reaction mixture includes 5µl of buffer 2X ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel purified and sub-cloned into the pGEM®-T Easy Vector System (Promega) as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and transformants were screened in a colony PCR for inserts of the correct size using GoTaq polymerase (Promega) with pCFD6_F and pCFD6_R primers ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments with lengths is above 500 bp to 2 kb were carried out with GoTaq enzyme (Promega) according to standard protocols ...
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bioRxiv - Synthetic Biology 2019Quote: ... The bands were purified using the Wizard® Plus SV Gel and PCR Clean-Up System kit (Promega), using the Quick Protocol specified by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The WGA products were purified using Wizard® SV Gels and PCR Clean-Up System (Promega, WI, USA). The DNA samples were used for library preparation ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and a 982bp fragment of Gad1 (Corresponding to nt 1015-1996 of NM_008077.2) were PCR-cloned into pGEM Easy T vector (Promega).
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bioRxiv - Neuroscience 2020Quote: One µl of bisulfite-converted DNA was subjected to PCR using the G2 Green Mix (Promega, Mannheim – Germany) with bisulfite-specific primers at temperatures and concentrations as indicated in Appendix 3 ...
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bioRxiv - Microbiology 2020Quote: ... PCR#2 product was ligated to pGEM-T easy vector or TOPO vector following the manufacturer’s instructions (Promega). After transformation in bacteria and plasmid amplification ...
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bioRxiv - Microbiology 2020Quote: ... The KAN PCR amplicon with compatible ends was ligated into the plasmid backbone using T4 DNA ligase (Promega). The resultant pEXP5/Kan vector was linearized using XbaI and Butters gp31FLAG was ligated into the plasmid for transformation into chemically competent BL21 cells ...
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bioRxiv - Cell Biology 2021Quote: A probe corresponding to the final 1078 bp of rps6ka3a (RSK2a; NM_212786.1) was generated by cloning a PCR-derived cDNA fragment into in pGEMT-Easy (Promega), linearising with ApaI (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The pooled amplicons were gel purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA) and the library was denatured and diluted following manufacturer’s guidelines (Illumina document no ...
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bioRxiv - Molecular Biology 2022Quote: RepEx-PCR-generated ~100x CAG repeats were inserted into a dual-luciferase reporter plasmid modified from pmirGLO (Promega) between firefly luciferase (FLuc ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... and the amplified DNA fragments were purified using Wizard SV Gel and PCR Clean-Up System (Promega #A9282). DIG-labelled RNA probes were synthesized using T7 or SP6 RNA polymerase and DIG RNA labeling Mix (Roche #11277073910) ...
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bioRxiv - Genetics 2022Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Genomics 2022Quote: ... PCR fragments were either sequenced directly or cloned using the pGEM-T Easy Vector System (Promega, Cat#: A1360) and Sanger sequenced to resolve insertion characteristics ...
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bioRxiv - Genomics 2019Quote: ... PCR for the detection of Y-linkage was performed with GoTaq® Hot Start Polymerase (Promega, cat# M5005) and primers were designed to target exons (for gene Y-linkage tests ...
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bioRxiv - Biochemistry 2019Quote: ... The N-terminal 312 aa Halo tag sequence was PCR amplified from His6HaloTag® T7 Vector pH6HTN (Promega) as a 5’ SpeI ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed for CGRP with GoTaq® qPCR Master Mix (Promega) using the DNA-binding dye ...
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bioRxiv - Genetics 2019Quote: ... and the linearized vector was purified by Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The pure linearized vector and the elpc-2 promoter were fused using an In-Fusion HD Cloning Kit (Takara ...
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bioRxiv - Genomics 2019Quote: ... PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega, Madison, WI, cat# M3005), 10 mM dNTPs ...
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bioRxiv - Plant Biology 2019Quote: ... Amplicons of correct size were gel purified with Wizard® SV Gel and PCR Clean-Up System (Promega). Amplicons were cloned into pDONR/Zeo (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... A PCR reaction was performed with 0.5 U of GoTaq DNA Polymerase in GoTaq Buffer (both from Promega) supplemented with primers and dNTPs to a final volume of 20 µL ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was synthesized by in vitro transcription of the PCR products using the T7 RNA polymerase (Promega kit). Then ...
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bioRxiv - Developmental Biology 2019Quote: ... This yielded a PCR product of 243 base pairs which is digested with restriction enzyme Bcl1 (Promega, R6651) in the presence of the G>A point mutation (i.e ...
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bioRxiv - Genomics 2020Quote: ... Obtained products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA), and subsequently cloned using the pGEM-T Easy Vector System (Promega ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed in 15 µl reactions using 2x GoTaq DNA Polymerase master mix (Promega Cat. No. M3008) with 5 ng of gDNA and 500 nM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was extracted from the excised agarose using a PCR product purification kit (Promega, Madison, Wisconsin, USA), following the manufacturer’s instructions for agarose-embedded DNA ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... The three fragments were cleaned up using the Wizard® SV Gel and PCR Clean-Up System (Promega) and subsequently fused by overlapping PCR ...
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bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
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bioRxiv - Cell Biology 2020Quote: ... and gel extractions were performed with the Wizard SV Gel and PCR clean-up kit (Promega, Madison, USA). Sequencing of plasmids was performed at the Massey Genome Centre ...
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bioRxiv - Pathology 2021Quote: ... One µL of PCR product was employed to produce the transcripts with the T7 RNA polymerase (Promega, USA), setting a 20 µL-reaction for 2 hours at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Positive PCR products were purified with the ReliaPrep™ DNA Clean-Up and Concentration System (Promega, Madison, WI). Genomic libraries were constructed with the Nextera XT DNA Sample Preparation kit (Illumina Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... Three μL of purified A-tailed PCR product was then ligated into pGemT®-T vector (Promega Corporation) following guidelines recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Genomics 2020Quote: ... PCR oligonucleotides contained additional sequences complementary to Bgl II restriction endonuclease digested pGL3-promoter plasmid (Promega, Southampton, UK) for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products from the unique regions of her9 were cloned into the pGEMT-easy vector (Promega, Madison, WI). Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB ...
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bioRxiv - Molecular Biology 2021Quote: 1 uL of pUG cDNA was then input into a 20 uL GoTaq PCR reaction (Promega; Cat # M7123) with the first adapter specific primer (Shukla et al. ...
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bioRxiv - Genetics 2020Quote: ... pGEM vector containing the desired PCR product was transformed into competent JM109 E.coli cells (Promega, Madison, WI, USA). Colonies were evaluated using blue-white screening and PCR with M13 universal primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR (qPCR) from 10ng of gDNA was performed using GoTaq qPCR Master Mix (Promega, A6001) on a TaqMan Viia 7 Real-Time PCR System ...