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Citations for Promega :
2101 - 2150 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously26 ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously ...
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bioRxiv - Microbiology 2022Quote: All isolates were heat-extracted at 95°C for 20 minutes for polymerase chain reaction (PCR) using GoTaq (Promega M7132) and 8F and 1492R primers to amplify the whole 16S rRNA gene (4) ...
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bioRxiv - Molecular Biology 2019Quote: ... The 3.6 kbp human CDC37 promoter region was PCR amplified and subcloned into a pGL3-luciferase vector (Promega, Madison, WI) using KpnI and BamHI ...
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bioRxiv - Physiology 2019Quote: Relative expression levels of Fgf23 were determined by qRT-PCR using 2 μl synthesized cDNA and the GoTaq qPCR Master Mix (Promega) on a Rotor-Gene Q (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... The cells with successful incorporation in the genomic locus of EGFP or TagRFP were screened by PCR using GoTaq Polymerase (Promega).
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bioRxiv - Genetics 2019Quote: ... genomic sequence downstream of the Nse4 gene was PCR amplified (JP414 and JP415) and inserted to the pGEM-Easy vector (Promega); 3 ...
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bioRxiv - Genetics 2020Quote: PCR used to detect the viral cDNA was performed using the GoTaq® Green Master Mix (Promega, Madison, WI, USA) under the following conditions ...
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bioRxiv - Genetics 2020Quote: ... and the mitogenome was extracted from the excised band by using the Wizard SV Gel and PCR Clean-up System (Promega).
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bioRxiv - Molecular Biology 2020Quote: Purified PCR products which amplified specific on-target or off-target sites were inserted into a T-easy vector (Promega) and transformed into DH5-α bacterial cells ...
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bioRxiv - Cancer Biology 2020Quote: ... were PCR amplified and cloned into either pGL3-Promoter firefly luciferase reporter vector or pGL3-Basic luciferase reporter vector (Promega). Constructs were verified by Sanger sequencing ...
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bioRxiv - Genetics 2020Quote: ... An 812 bp region (3L: 15825979..15826790) spanning the candidate mutated region in DCP2 was PCR amplified and ligated in pGEM-T vector (Promega) to generate the pGEM-T-812 clone ...
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bioRxiv - Biochemistry 2020Quote: The Arg5,6-coding region or a fragment of it was amplified by PCR and cloned into pGEM4 (Promega, Madison, WI) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega, Madison, WI) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Biochemistry 2020Quote: ... PCR products were resolved on a 24% TBE gel and visualized using ethidium bromide or cloned into pGEM-T (Promega) and Sanger sequenced ...
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bioRxiv - Plant Biology 2020Quote: ... Gene PCR products of 200-300 bp were labelled with 32P-dCTP following the prime-a-gene labelling kit instructions (Promega) and used as probes (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2019Quote: ... The coding region of Caspase9 was PCR-amplified using pX330 as a template and inserted into the pSP64 Poly(A) vector (Promega). Vector were digested and linearized with EcoRI ...
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Neuroscience 2019Quote: ... The coding region of Caspase9 was PCR amplified using pX330 as a template and inserted into the pSP64 Poly(A) vector (Promega). Vectors were digested and linearized with EcoRI ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µl of each reaction was used as template in subsequent PCR amplification reactions in a final volume of 50 µl with the GoTaq Green Mastermix (Promega) in the presence of 0.2 M of each oligo under following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR product were subsequently disgested by SphI and EcoRI prior cloning into a pGEM®-T Easy Vector (Promega #A1360). Then ...
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bioRxiv - Neuroscience 2019Quote: ... was amplified via high-fidelity PCR and inserted in-frame along with a firefly luciferase coding sequence (amplified from psiCHECK-2, Promega) with a P2A sequence (Holst et al ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was performed in a 25 µl reaction volume with 12.5 µl of GoTaq ® Green Master Mix (Promega, USA), 1 µl of 100 ng/µl gDNA ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplicons were purified using Qiagen PCR Cleanup (Cat no. 28104) and pooled with pTK nanotag reporter vector with T4 DNA ligase (Promega) and BsmBI (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... Approximately 100bp of the surrounding sequence was amplified by PCR from LX2 cells and cloned into the XhoI site of the pGL3+promoter vector (Promega) with corresponding empty vector as control ...
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bioRxiv - Cell Biology 2020Quote: ... −2784 bp from the start codon to +688 bp from the stop codon) was amplified from Arabidopsis genomic DNA by PCR and integrated into pGEM-T Easy vector (Promega). First ...
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bioRxiv - Plant Biology 2021Quote: ... For SlTPD1 a 284 bp DNA fragment from the 5’ coding region was amplified by PCR using cDNA from flowers and cloned into the pGEM-T Easy vector (Promega). For TomA5B and SlSDS genes ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Cell Biology 2019Quote: ... 5’ GA CTC GAG GTT GCA CCT AGT GGA T 3’ The PCR product was first cloned into pGEM-T easy vector (Promega), verified by sequencing and subsequently cloned into pGEX5.1 vector using the EcoRI and XhoI sites ...
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bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
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bioRxiv - Genetics 2021Quote: ... Bands purification were made with Wizard® SV Gel and PCR Clean-Up System (Promega, 2800 Woods Hollow Road·Madison, USA). Next ...
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bioRxiv - Genetics 2021Quote: ... 3ul of gel extracted PCR product was used for TA cloning with the pGEM-T Easy Vector System (Promega, A1360) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The PCR products and restriction enzyme digestions were purified by agarose gel electrophoresis followed by processing with the Wizard SV Gel and PCR clean up system (Promega). Restriction enzymes were purchased from Fermentas ...
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bioRxiv - Microbiology 2020Quote: ... The genomic region flanking the Cas9 target site from each ΔNAT10 cell line was PCR amplified and cloned into the XbaI/SalI sites of pGEM-3zf+ vector (Promega). 10+ bacterial cell clones of pGEM-genomic-region-plasmid from each CRISPR-knockdown cell clone were isolated for Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence of REST/NRSF ZF1-8 were amplified by PCR using cDNA as templates and cloned into the pTNT vector (Promega) between the EcoRI and XbaI sites ...
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bioRxiv - Molecular Biology 2021Quote: ... the sequences containing putative NRSE motifs were amplified by PCR from the human genomic DNA and then subcloned into the pGEM-T Easy vector (Promega). To generate plasmid templates for the mutated probes ...
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bioRxiv - Molecular Biology 2021Quote: ... 3’UTR PCR products were directionally cloned downstream of the Renilla luciferase open reading frame (ORF) of the psiCHECK2 vector (Promega) that also contains a constitutively expressed firefly luciferase gene ...
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bioRxiv - Genetics 2020Quote: ... 1-2 μL of cDNA was used for 25 μL PCR reactions using the GoTaq Hot Start Master Mix (Promega). Cycling parameters consisted of an initial denaturation of 95°C for 2 min. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... Genome editing efficiency was checked by PCR on genomic DNA from the transfected population with the GoTaq G2 polymerase (Promega) using primers surrounding the 210 bp deleted region ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously [25] ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups were screened for the deletion by classical genotyping PCR with the GoTaq R2 Hot Start Green Master Mix (Promega) with fw (5’-CCTCGGAAGCTGCCTAAGAT-3’) ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized by using qScript cDNA Synthesis Kit (QuantaBio, Beverly, MA) and PCR was performed with GoTaq qPCR Master Mix (Promega) with HPRT as the control gene ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR fragments were purified over gel and the DNA was recovered using the Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified on a 1% agarose gel and ligated into a pGEM-T Easy Vector Systems (Promega) plasmid followed by transformation in E ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were visualized using agarose gel electrophoresis and subsequently cloned into a pGEM-T easy cloning vector (Promega, Australia). Blue/white colonies were screened using standard techniques and a test digestion with endonuclease restriction enzyme EcoR1 was carried out to confirm insertion ...
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bioRxiv - Neuroscience 2022Quote: ... Templates for RNA FISH probes were amplified by PCR from genomic DNA (Table S3) and cloned into pGEM-T Easy (Promega) or pCRII-TOPO ...