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Citations for Promega :
2251 - 2300 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... All cells were confirmed to be free of mycoplasma (PCR-based method) and authenticated by Short Tandem Repeat (STR) analysis using the GenePrint® 10 System (Promega, Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stably transduced with a lentivirus-based expression construct generated by cloning a polymerase chain reaction (PCR)-amplified DNA fragment encoding luciferase from a pGL4.10-lucIIplasmid (Promega, Sydney, Australia) into a pLenti CMV Hygro DEST vector (Addgene ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed in a final volume of 25 µL with 12.5 µL of GoTaq Green Mastermix (Promega, Madison WI, USA), 9.5 µL of water ...
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Genetics 2023Quote: ... and cloned the PCR products into JM109 competent cells using pGEM®-T Easy Vector Systems (Promega Corporation, Madison, WI, USA). Transformed cells were amplified and Sanger sequenced at the Genomics Facility ...
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bioRxiv - Immunology 2023Quote: ... 25 ng of c-DNA was used per reaction in each well containing the 2X SYBR green PCR master mix (Promega, USA) along with appropriate primers ...
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bioRxiv - Microbiology 2023Quote: ... Marker genes in isolated DNA were polymerase chain reaction (PCR)-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, Wisconsin) and 515 F (5′-GTGCCAGCMGCCGCGGTAA-3′) ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Genomics 2024Quote: ... 1x SYBR Green I] and calculated from a standard dilution series of a PCR product (quantified with QuantiFluor® dsDNA System, Promega with a DeNovix DS-11 spectrometer ...
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bioRxiv - Immunology 2024Quote: ... A total of 10 ng of cDNA was used for quantitative PCR in a total volume of 10 µl with GoTaq® qPCR Master Mix (Promega) and specific primers ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... The pCMV5D_HA-WDR44 plasmid was purchased from MRC PPU Reagents and Services (DU26855) and a pFN21A-Halo-KIAA0100 plasmid (which served as a PCR template) was purchased from Promega (FHC00016). GFP-Rab8 and GFP-Rab11 were kind gifts from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Neuroscience 2024Quote: ... One µL of lysate was then added to make 20µL PCR reactions with GoTaq G2 Green Master Mix (Promega, Cat# M7823) and 1µM forward and reverse primers (Table 1) ...
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bioRxiv - Cancer Biology 2024Quote: The target sites for each crRNA were PCR amplified from genomic DNA extracted from tumor-bearing lungs using GoTaq Green® Mastermix (Promega) and the following primer pairs:
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bioRxiv - Genetics 2024Quote: ... Resulting amplicons were purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega cat no. PR-A9281) then cloned into TOPO TA pcR2.1 (Invitrogen cat no ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from 1 ml concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [74] ...
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bioRxiv - Cancer Biology 2024Quote: ... and the expression of the CRISPRi targeted lncRNA genes was assessed through Real-Time PCR with the GoTaq® qPCR Master Mix (Promega). Primers sequences are listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2023Quote: ... for either WT or mutant were PCR amplified from zebrafish shoc2 complementary DNA (cDNA) and cloned into the pCS2+ vector (Promega, WI). The capped mRNAs were synthesized with the mMessage mMACHINE SP6 transcription kit (Thermofisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 500–800 nt amplicons were amplified from genomic DNA from individual insertion lines through single fly PCR using GoTaq green master mix (Promega M7122).
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Microbiology 2020Quote: ... and A-tailed following the protocol for cloning blunt-ended PCR products described in pGEM®-T Easy Vector Systems Technical Manual (Promega, UK). An aliquot of A-tailed PCR products was cloned into pGEM-T Easy and then transformed into E ...
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bioRxiv - Cell Biology 2020Quote: ... according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega, Madison, WI) and the following primer pairs (see also ‘Primer and sgRNA sequences’):
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bioRxiv - Genetics 2020Quote: ... The appropriate band (1595 bp) was excised and purified as recommended by the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). The blunt end PCR product was A-tailed with a 20 min incubation step at 72 °C by adding 0.2 mM dATP ...
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bioRxiv - Developmental Biology 2020Quote: ... primers were used to amplify the PCR product (fwd 5’-GCTGTFATAGGGTGGAGGTG-3’, rev 5’GCTATCAACGCCATTGTGAA-3’) using 1X GoTaq Green (Promega, Madison, WI) with a final primer concentration of 0.2uM ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase Chain Reaction (PCR) targeting recA was performed in 50 µl volumes using 10 µl of 5X buffer (Promega, Madison, WI, USA), 7 µl of 25 mM MgCl2 (Promega ...
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bioRxiv - Genomics 2019Quote: ... The A-tailed PCR products were then ligated to pGEM®-T Easy Vector using pGEM®-T Easy Vector Systems (Cat. A1360, Promega). Positive colonies were selected for Sanger sequencing ...
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bioRxiv - Genetics 2019Quote: ... DNA fragments were separated by agarose-gel electrophoresis and purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA). Sequence authenticity was confirmed at the Massey Genome Centre ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Raw PCR products were then used to generate digoxigenin-labeled RNA probes using a T7 RNA in vitro transcription kit (Promega / Life Technologies). RNA was ethanol precipitated and resuspended in water to analyze on a Nanodrop ...
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bioRxiv - Bioengineering 2021Quote: ... Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712, Promega, France) according to the manufacturer’s recommendations with the Nix-833 published primers 25 and primer EM1926-EM1927 (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... or the region between abaR and the abaI (for the abaI fusion) were amplified by PCR and ligated in pGEM®-T Easy using the pGEM®-T Easy Vector System (Promega). The resulting plasmids and the promoterless luxCDABE operon were digested with KpnI and BamHI and ligated in order to introduce the lux operon downstream of the predicted promoter of abaM or abaI ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The PCRs were performed in a final volume of 20 μl of amplification mix containing 1 U of GoTaq polymerase (Promega, Madison, WI), 1× GoTaq buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... RiMCO1 and RiMCO3 full-length cDNAs were obtained by PCR amplification of the genes cloned in pGEM-T easy vector (Promega, Madison, USA), using the corresponding primer pairs (Table S1) ...
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bioRxiv - Cancer Biology 2022Quote: Synthesized cDNA as described above was used to measure levels of unspliced and spliced XBP1mRNA were measured by regular PCR performed with GoTaq Green Master Mix (Promega cat#: M7123) on a T100 Thermo Cycler (Bio-Rad cat# ...
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bioRxiv - Synthetic Biology 2022Quote: ... Amplified DNA products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Germany, Art. No. A9282). In all agarose gels ...
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bioRxiv - Physiology 2019Quote: An aliquot of cDNA obtained by reverse transcription was amplified in a PCR reaction with GoTaq Green Master Mix (Promega, Milan, Italy). For each pair of primers ...
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bioRxiv - Physiology 2019Quote: ... +1 corresponding to start of the first exon) was amplified by PCR and cloned into the pGL4.21-Luc vector (Promega, Madison, WI, USA). Systematic promotor truncations were generated using the Q5 Site-Directed Mutagenesis Kit according to manufacturer’s instructions (New England Biolabs ...
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bioRxiv - Cancer Biology 2019Quote: ... The new-synthesized cDNA products were further used as the templates of real-time quantitative PCR (qPCR) in the GoTaq®qPCR Master Mix (Promega, USA) containing each pair of the indicated primers (with specific nucleotide sequences as listed in Table 1) ...
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bioRxiv - Genomics 2019Quote: ... All PCR products were checked on 2% agarose gels and then cleaned with the Wizard SV Gel and PCR Clean-up System from Promega (Madison, Wisconsin) and sent off for Sanger-sequencing along with the amplification primers to Molecular Cloning Laboratories (San Francisco ...
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bioRxiv - Genomics 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Immunology 2022Quote: ... that cover the promoter region and proximal portion of the first exon of the gene was PCR amplified with GoTaq DNA Polymerase (Promega, Madison, Wisconsin) from bisulfite-converted DNA using the primer listed in Table S3 ...
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bioRxiv - Physiology 2022Quote: ... agarose gel and the appropriate bands were cut out and purified according to the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). A 20 min A-tailing step (72 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified using the Wizard SV Gel and PCR Clean-R19 purification systems following the instructions by the manufacturer (Promega, Madison, USA). The positive PCR amplicons were bidirectionally sequenced and gathered using BioEdit software (Hall ...
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bioRxiv - Neuroscience 2023Quote: ... the 5’-flanking region of mouse TrkA gene (79799-80637 of AC161454.3) was PCR-amplified from the genomic DNA of C57BL6J mice and subcloned into the pGL2-basic vector (Promega, Fitchburg, WI, USA). The following primers were used for PCR amplification ...