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Citations for Promega :
2051 - 2100 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... DNA were purified using the Wizard® SV Gel and PCR Clean-Up System protocol (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with ApaI and XhoI and then purified (Wizard® SV Gel, Promega, WI, USA). The eluted fragment was ligated downstream of an inducible acetoacetate decarboxylase promoter (Padc ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from agarose gel was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of DNA was taken as template in a 25 μl PCR reaction using the Go Taq DNA polymerase (Promega) according to the provider’s recommendation ...
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bioRxiv - Developmental Biology 2021Quote: ... fragment of Snai2 coding sequence (from nucleotides 165 to 971) was PCR-amplified from quail cDNA and cloned in pGEM-T Easy vector (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Mtfp1 and Gnpat 3’UTRs and Tnksbp1 and Gnpat CDS were amplified by PCR from MIN6 cDNA and subcloned into pmirGLO (Promega), downstream the Firefly ORF ...
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bioRxiv - Molecular Biology 2021Quote: ... and the desired bands were recovered from the gel using Wizard® SV Gel and PCR Clean-Up System (Promega). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time PCR was performed on an ABI Prism 7900 detection system using Gotaq master mix (Promega, Madison, WI, USA). Expression relative to the housekeeping gene TBP was calculated using the delta(d)Ct method and expressed as 2^(-dCT ...
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bioRxiv - Developmental Biology 2021Quote: ... the proximal promoter region (−299/−1) of MyoG was isolated by PCR and inserted into the pGL3-basic vector (Promega). The DNA fragments of PME1 and PME2 in the MyoG promoter region were also amplified and inserted into the pGL3-γ-actin-TATA vector ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR was performed on an Applied Biosystems StepOne Plus Real-Time PCR system using GoTaq qPCR Master Mix with SYBR Green (Promega) and the primers listed in Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... and random hexamers were used for reverse transcription and PCR amplification was carried out using 25 cycles and GoTaq polymerase (Promega) with linker specific primers ...
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: Viral RNA was extracted from 0.2 mL of cobas PCR Media using Maxwell® 16 instrument (Promega, Madison, WI, USA) for final elution in 30μL ...
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bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA from all KO cell lines was extracted and the region flanking the Cas9 target site from each ΔPUS cell line PCR amplified and cloned into the EcoRI and HindIII sites of pGEM-3zf vector (Promega). The primers used to amplify the region flanking the Cas9 target sites were the following ...
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bioRxiv - Molecular Biology 2021Quote: A conventional PCR targeting the hsp65 gene was conducted using the GoTaq® Green Master Mix (Promega, Madison, Wisconsin, USA) in a final reaction volume of 13μl comprising 6.25μl of 2X GoTaq Hot Start Green Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... All DNA extractions were done with the Wizard® SV Gel and PCR Clean Up system kit (Promega, Madison, USA).
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bioRxiv - Cell Biology 2020Quote: ... Generated WIP1 and WIP1-ΔκB promoter PCR products were cloned into pGL3-basic luciferase reporter vector (Promega, Madison, WI, USA) using XhoI and BglII restriction enzyme sites and designated as pGL3-WIP1 and pGL3-WIP1-ΔκB ...
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bioRxiv - Microbiology 2019Quote: For all qRT-PCR reactions 1µg of total RNA was treated with DNase using RQ1 RNase-free DNase (Promega, USA) and transcribed into cDNA (RevertAid First Strand cDNA Synthesis Kit ...
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bioRxiv - Genetics 2019Quote: ... Beads were washed four times with IP buffer and bound nucleic acids were isolated using the Wizard SV gel and PCR clean-up kit (Promega). Collected DNA fragments were quantified using RT-PCR as described above with the following primers ...
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bioRxiv - Molecular Biology 2019Quote: Expected products of SDDV ATPase fragment amplification (135 bp) obtained from the above PCR assays were cloned into pGEM-T easy vector (Promega) and transformed into E ...
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bioRxiv - Plant Biology 2019Quote: ... 40 μL of supernatant was then transferred from each sample to PCR strip tubes and mixed with 10 μL Renilla luciferase lysis buffer (Promega). Prepared samples were then stored at −20°C until luciferase assays were run ...
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bioRxiv - Biophysics 2019Quote: ... the same DNA was combined with a PCR fragment amplified from cZP1full/pGEM57 to generate a 6His-tagged full-length cZP1 ORF in pSI (Promega). The pHLsec3 construct expressing C-terminally 6His-tagged mZP1-N1M1-A141 was generated by PCR ...
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bioRxiv - Microbiology 2019Quote: ... The linear amplification product was purified using the Promega Wizard SV Gel and PCR Clean-up System (Promega Corporation, A9282), and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Cell Biology 2019Quote: A cDNA fragment corresponding to nt995-1537 of mouse trip6 cDNA (GenBank accession number NM_011639.3) was generated by PCR and subcloned into the pGEM-T Easy Vector (Promega, Madison, WI). Radiolabeled riboprobes were generated by using [35S]UTP (Hartmann Analytik ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The allelic frequencies of the long and short alleles in the northern Queensland population were found by taking 14 samples of 56 to 92 flies and individually PCR genotyping all flies in the sample using GoTaq (Promega). Primer sequences ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were genotyped by extracting DNA from tail clippings with Extracta DNA Prep for PCR – Tissue (Quanta Biosciences) and specified products amplified using either GoTaq Green Mastermix (Promega) or 2x KAPA buffer ...
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bioRxiv - Biochemistry 2020Quote: ... or ∼30 ng (Proevolidine and Proxanthoxycyclin E gDNA) of the A-tailed PCR product were ligated into 50 ng of the pGEM-T Easy vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was probed with a 400 bp 32P-labelled PCR product of the TbCls 3’UTR generated with the prime-a-gene labelling system (Promega). Primers used for PCR were ...
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bioRxiv - Bioengineering 2020Quote: ... The second round of PCR was run with 10μL GoTaq Green Master Mix (Promega Biosciences LLC, San Luis Obispo, CA), 4.2μL of water ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted using HotSHOT (hot sodium hydroxide and tris) and PCR was performed using GoTaq green master mix (Promega) (Truett et al ...
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bioRxiv - Cell Biology 2021Quote: ... ShhN dsDNA coding for residues 1-198 of murine Shh was obtained by PCR using Pfu polymerase (Promega cat # M7741) and the following primers ...
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bioRxiv - Neuroscience 2020Quote: A DNA fragment bracketing the proximal promoter and transcription start site of the Iqsec3 gene (–280 to +36) was PCR-amplified from mouse genomic DNA and subcloned into the pGL3-Basic vector (Promega). Full-length mouse Npas4 cDNA was subcloned in pcDNA3 (Gibco-Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: Total mRNAs used for qRT□PCR experiments were isolated from Chlamydomonas using the Eastep Super total RNA extraction kit (Promega), and cDNA was synthesized with the PrimeScript TMRT Master Mix kit (TaKaRa) ...
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bioRxiv - Plant Biology 2020Quote: ... Amplified products were sent to the Australian Genome Research Facility for sequencing to confirm identity following the use of the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Plant Biology 2020Quote: ... or a 1007-bp fragment of CaUNI (182–1188 from ATG) amplified by PCR and cloned into the pGEM-T Easy vector (Promega). Transcription of probes was carried using SP6 and T7 polymerases ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR reaction was carried out in a 50 μl reaction volume containing 1.25 U GoTaq Flexi DNA Polymerase (Promega, USA), 10 μl of 5X Flexi Reaction Buffer (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Two allelic replacement vectors—pFtsH1G489G carrying two synonymous “shield” mutations and pFtsH1G489C carrying a further G to T mutation at base 1465 (Figure 1A)—were created by cloning a PCR amplified segment of Pfftsh1 into pGEM-T Easy (Promega). Quickchange XL (Clontech ...
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bioRxiv - Bioengineering 2020Quote: ... The second round of PCR was run with 10μL GoTAQ Green Master Mix (Promega Biosciences LLC, San Luis Obispo, CA), 4.2μL of water ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at 37°C and DNA was extracted using the Wizard SV gel and PCR cleanup system (Promega). Input and immunoprecipitated DNA was subjected to quantitative PCR using the oligonucleotides shown in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cohesive ends were generated by restriction digestion of this PCR product using Eco R1 and Not1 and ligated using T4 DNA ligase (Promega) with Eco R1 ...
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bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Microbiology 2022Quote: ... Clonal line selection was then performed with newly acquired mutant strains before molecular verification by diagnostic PCR using GoTaq DNA polymerase (Promega) and multiple sets of primers (Tables S9 and S10) ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR amplified inserts were run on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the 2206 bp immediately upstream of the SCL30a start codon were PCR-amplified (S7 Table) from genomic DNA and subcloned into the pGEM vector (Promega), where the eGFP-GUS segment isolated from the pKGWFS7 vector [69] using the SacII/NcoI restriction sites was fused at the 3’ end of the SCL30a promoter sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... the ∼600 bp homology sequence to the target site was amplified by overlapping PCR and inserted into a Sal I and Not I digested pGL3-Basic plasmid (Promega). Cas9 and donor plasmids were co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Luciferase constructs were obtained by PCR amplifying the 3’UTR regions from cDNA extracts and cloning them into a modified phRL-CMV vector (Promega), as previously described (Favereaux et al. ...