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Citations for Promega :
2201 - 2250 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... then probed with goat α-mouse HRP-conjugated secondary antibody (Promega, diluted 1:10000 in Blocking Buffer) and finally washed 3 times in TBS-Tween ...
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bioRxiv - Biochemistry 2021Quote: ... 600 μL 50 mM triethylammonium bicarbonate (TEAB) buffer and 0.5 μg/μL trypsin (sequencing grade, modified, Promega) was added (overnight ...
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bioRxiv - Biophysics 2020Quote: ... supernatants were harvested for virion purification and monolayers were lysed in cell culture lysis buffer (CCLR, Promega) for immunoblotting ...
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bioRxiv - Microbiology 2020Quote: ... Lysis buffer (20 μL) was used for luciferase activity measurement with the Luciferase Assay System (Promega, E151A) in a BioTek Synergy 2 reader.
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bioRxiv - Biochemistry 2021Quote: ... prior to the addition of 80μl digestion buffer (10% 2,2,2-Trifluoroethanol (TFE)/100 mM NH4HCO3) containing 4μg Trypsin-gold (Promega, V5280) and 4μg Lys-C (Wako ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells from the host shutoff assays above were harvested and lysed in passive lysis buffer (Promega) for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 μg of mitochondrial protein in triplicate was incubated in GTPase Buffer (Promega #V7681; Madison, WI, USA) with 10 μM GTP and 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... After the addition of 1x Nano-Glo Lysis Buffer and 1:1000 Nano-Glo substrate (Promega, USA), relative light units (RLU ...
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bioRxiv - Physiology 2023Quote: ... Thirty μg of total protein extract was isolated from the VSMCs using 1X passive lysis buffer (Promega) and was mixed with radio-labeled oligo for binding ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... using PLB buffer and luminescence activity was measured with the Nano-Glo® Luciferase Assay System (Promega).
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bioRxiv - Neuroscience 2023Quote: ... or 50 nM) in buffer A with 0.4 U/µL RNase inhibitor (RNasin Plus RNase Inhibitor, Promega). Biotinylated (AAAAAA)4 and TERRA are negative and positive controls ...
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Genetics 2023Quote: ... Additional 140 uL of lysis buffer and 40uL of proteinase K (20mg/ml, Promega, Cat. No. MC5005) were added ...
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bioRxiv - Cancer Biology 2024Quote: Protein pellets were dissolved in digestion buffer and digested by 1% ProteaseMAX detergent (Promega, Madison, WI, USA) and Trypsin/Lys-C Mix (Promega ...
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bioRxiv - Systems Biology 2024Quote: ... UA buffer was exchanged with 50 mM ammonium bicarbonate and sequencing-grade trypsin (Promega, Madison, WI, USA) was added at an enzyme to protein ratio of 1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were washed twice with warm PBS and lysed using 100 μL passive lysis buffer (Promega, E194A) per well (24-well format ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were washed with PBS before harvest and harvested with 1X Passive Lysis Buffer (5X, Promega #E1941) diluted in water ...
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bioRxiv - Plant Biology 2023Quote: ... 0.5 μl of the DNA extract was added to 10 μl of 5X GoTaq Reaction Buffer (Promega) and 1 μl of each primer (10 μM ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with methanol TEAB buffer followed by overnight Trypsin (1 μg, Promega) digestion at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... cells in each well were first lysed with 50μL ice-cold 1X passive lysis buffer (Promega E1941), allowed to incubate on an orbital shaker for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... lysed by rocking for 15 min at room temperature in 500 µl of passive lysis buffer (Promega), and clarified by centrifugation for 2 min at 21,000 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... cells are washed with PBS1X and then lysed with 250 μl of 1X Passive Lysis Buffer (Promega). Firefly and renilla luminescences were then monitored with Dual-Glo Luciferase Assay kit (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed with 100μL of lysis buffer provided with the Dual-Glo Luciferase assay kit (Promega). The GloMax® 96 Microplate Luminometer was used to measure the activity of Firefly and Renilla luciferases according to the manufacturer’s protocol (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... Four transiently bombarded leaf events were pooled together and crushed in Luciferase Cell Culture Lysis buffer (Promega). The extract was centrifuged at 12,000 rpm for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed at 4 °C in RIPA buffer containing protease and phosphatase inhibitors cocktail (Promega/Sigma). Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestion buffer (50 mM TEAB pH 8.0) containing sequencing-grade modified trypsin (1/25, w/w; Promega) was added to the S-trap column and incubate for 1h at 47 °C ...
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bioRxiv - Microbiology 2024Quote: ... Thawed cell pellets were resuspended with 0.5 ml of 2% CTAB Buffer (Promega Corporation, Madison, Wisconsin, USA), transferred to a Lysing Matrix E tube (MP Biomedicals ...
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bioRxiv - Developmental Biology 2024Quote: ... transfected cells in 12-well plates were digested by 100 μL Passive Lysis buffer (Promega, Cat. E1941) per well for 15 min ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the reconfirmed hits was determined by using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) on A549 human lung carcinoma cells [40] ...
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bioRxiv - Cancer Biology 2020Quote: ... cell viability was measured using the CellTiter 96® Aqueous One Solution Cell Proliferation Assay according to manufacturer specifications (Promega, Madison, WI).
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bioRxiv - Neuroscience 2020Quote: ... samples were digested with 50 μl of 50 mM ammonium bicarbonate solution containing 5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
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bioRxiv - Genomics 2022Quote: ... All growth was collected using sterile plastic loops and placed directly into Wizard Genomic DNA purification kit Nuclei Lysis solution (Promega, reference A1120). The Wizard kit extraction protocol was then followed ...
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bioRxiv - Physiology 2022Quote: Assays involving a range of peptides from 14mer down to 6mers assessed total viable PC3 or PNT2 cells using a 96-well plate MTS colorimetric assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay, Promega, Southampton, UK), with absorbance measured at 492 nm ...
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bioRxiv - Physiology 2022Quote: ... or only administration (LNCaP) total viable cell numbers were measured colorimetrically (MTS) using a CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, Southampton, UK), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: MTS assays of HCoV-229E experiments were performed using the The CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, #G3582). In brief ...
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bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, 182 Madison, WI) at 4ºC ...
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bioRxiv - Cancer Biology 2021Quote: The effect of P2RX7B expression and inhibition on OS cell proliferation was assessed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, UK), TE85 ...
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bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
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bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...