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Citations for Promega :
2001 - 2050 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with 10 μl RQ1 RNase-Free DNase (Promega Cat. # 89836) at 37 °C for 30 min and 7 μl of 10x DNase Stop Buffer to denature the DNase at 65 °C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... and spleen samples that were extracted using the Wizard® Genomic DNA Purification Kit (Promega). Three samples from Victorian koalas that had previously been identified as containing recKoRV via long read sequencing were also included in initial testing (Tarlinton et al ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with DNAse (RQ1 Rnasa-Free Dnase, Promega, Madison, WS, USA) and retrotranscribed with FireScript KIT Reverse Transcriptase (Solis BioDyne ...
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bioRxiv - Physiology 2024Quote: ... samples were digested by adding 10 µL of 20 ng/uL sequencing grade trypsin (Promega) into the lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then incubated overnight at 37°C with 100 ng trypsin (sequence grade, Promega). The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were digested with trypsin (1/100, w/w, Sequencing Grade Modified Trypsin, Porcine; Promega) overnight ...
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bioRxiv - Biophysics 2024Quote: ... Each sample was diluted to 0.35 mg/ml to perform the Luciferase Assay (Promega E1500). Luciferase were thawed in a room temperature water bath ...
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bioRxiv - Biochemistry 2024Quote: ... samples were incubated overnight at 37°C with 500 ng sequencing grade modified trypsin (Promega). The supernatant containing the peptides were collected and dried using the SpeedVac.
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then digested with trypsin at a ratio of 1:50 (Promega #V5111) over night at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was digested overnight with 2.5 µl of 1 µg/µl trypsin (Promega V528A) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were harvested and assayed for luciferase (Luc Assay System with Reporter Lysis Buffer, Promega) in a Beckman Coulter LD400 plate reader.
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were incubated for 24 hours post-transfection before extraction with passive lysis buffer (Promega). Activity levels were measured for Firefly and Renilla luciferase using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction products were split into 10 μL aliquots and S1 nuclease 10x reaction buffer (Promega) was added to 1x concentration ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed in PBS and harvested in 100 μL of 1X passive lysis buffer (Promega). The Dual-Luciferase Assay Reporter Kit (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were washed with PBS and lysed with 100 μL of passive lysis buffer (Promega) and luciferase activity was measured using Bright-Glo Luciferase Assay reagents (Promega ...
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bioRxiv - Molecular Biology 2021Quote: 5–10 oocytes were lysed by vigorous shaking and pipetting in Passive Lysis Buffer (Promega, E1980), using 20 µl per oocyte ...
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bioRxiv - Synthetic Biology 2020Quote: ... that was reconstituted within trypsin re-suspension buffer (50 mM acetic acid; #V542A, Promega, WI, USA), and 39 μl or 49 μl 250 mM Tris buffer ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were resuspended in 35 μl of reaction buffer (0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Cells were transduced by PV for 48 hours before lysis with cell culture lysis buffer (Promega). Luciferase luminescence was read on a FLUOstar Omega plate reader (BMF Labtech ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: Three wells of a 6-well plate were lysed were lysed in Reporter lysis buffer (Promega) for 10 min on ice before centrifugation at 17,000 g ...
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bioRxiv - Biochemistry 2021Quote: ... Media were recovered while cells were rinsed in PBS and lysed in Passive Lysis Buffer (Promega). Media was directly subjected to Western Blotting ...
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bioRxiv - Neuroscience 2020Quote: ... cells were rinsed with PBS and incubated with the reporter lysis buffer (Promega, Madison, WI, USA) for 15min at rt ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: 100000 cells or 200 mg tissue were lysed in 100-500 μl Passive Lysis Buffer (Promega) for 15 min at room temperature on an orbital shaker ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed once with PBS and lysed in 40μl of 1 x CCLR buffer (Promega) for 10 min on a rocking plate at RT ...
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bioRxiv - Microbiology 2020Quote: ... and were then lysed in 100μl of cell culture lysis buffer (part of the Promega kit) diluted to 1x in ddH2O ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were washed with 1X PBS and then lysed with 1X Passive Lysis Buffer (Promega) for 10 mins at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and the cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) followed by three freeze-thaw cycles ...
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bioRxiv - Microbiology 2021Quote: ... and the cells were lysed by the addition of 30 μl of passive lysis buffer (Promega) and one freeze-thaw cycle ...
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bioRxiv - Microbiology 2021Quote: ... The infected cells were collected and lysed with 100 μl of 1× passive lysis buffer (Promega). The samples were sonicated for 30 seconds before centrifugation and 5 μl of the supernatants were collected for luciferase expression reading by the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Microbiology 2021Quote: ... then resuspended in 100 ul of SA-RNP lysis buffer supplemented with 80U RNAsin (Promega, USA) and incubated with 30 ug of renatured RNA with overhead rotation at 4°C for 2.5h ...
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bioRxiv - Microbiology 2020Quote: Cells were washed with PBS and lysed in 200 μL of 1X Passive Lysis Buffer (Promega). After gentle shaking for 15 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed with Passive Lysis Buffer (PLB) from the Dual-Luciferase Reporter Assay (Promega, USA), Luciferase Assay Reagent (LARII ...
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bioRxiv - Molecular Biology 2022Quote: Whole cell extracts from cultured cell lines were prepared using 1x GLO lysis buffer (Promega, E266A). One third volume of 4x Laemli buffer was added to one volume whole cell extract samples ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed in PBS and harvested in 100 µL of 1X passive lysis buffer (Promega). The Dual-Luciferase Assay Reporter Kit (Promega ...
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bioRxiv - Microbiology 2022Quote: ... the spent medium was removed and 60 μl of the cell lysis buffer reagent (Promega Inc.) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... Cells transduced with pLentiCMV-TurboGFP::Luciferase pseudoparticles were lysed after 48h with Luciferase-Lysis Buffer (Promega) and detected using Beetle-Juice Luciferase Assay according to manufacturer’s instructions and a Biotek Synergy 2 platereader ...
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bioRxiv - Biochemistry 2020Quote: ... The attached cells were lysed in 20 μl of 1× passive lysis buffer (1× PLB, Promega) at room temperature (RT ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... after which they were lysed by the addition of 40 μl of passive lysis buffer (Promega) and one freeze-thaw cycles ...
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bioRxiv - Synthetic Biology 2021Quote: ... The remaining cells were harvested and lysed with 30 μl of 1× passive lysis buffer (Promega).
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Full growth medium was replaced 24h after transfection and cells were lysed in lysis buffer (Promega) 48h after transfection ...
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bioRxiv - Microbiology 2020Quote: Supernatants were taken from cell medium and mixed with equal volume of 2 ×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Microbiology 2021Quote: ... PBS buffer having 1,000 volume diluted Nluc-specific furimazine substrate (Nano-Glo Luciferase Assay System, Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293 cells treated with 1 μM thapsigargin were lysed in 1X Passive Lysis Buffer (Promega) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoScientific ...
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bioRxiv - Microbiology 2021Quote: Supernatants were taken from cell medium and mixed with equal volumes of 2×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... then 50 µL of drug buffer with RLuc substrate coelenterazine h (Promega, 5 µM final concentration) was added per well ...
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bioRxiv - Immunology 2020Quote: ... containing 10 μl/well of RNA catch buffer (10mM Tris pH7.4 supplemented with RNasin (Promega, UK)) ...