Labshake search
Citations for Promega :
2051 - 2100 of 3140 citations for Laemmli Sample Buffer 2X Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were sampled by washing twice in PBS and lysed in Glo Lysis Buffer (Promega, E2661) for 5 min at RT ...
-
bioRxiv - Genomics 2023Quote: ... Cells were sampled by washing twice in PBS and lysed in Glo Lysis Buffer (Promega, E2661) for 5 min at RT ...
-
bioRxiv - Genomics 2023Quote: ... Cells were overlaid with pseudovirus for 48 h before lysis with cell culture lysis buffer (Promega). We determined luciferase luminescence on a FLUOstar Omega plate reader (BMF Labtech ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the cells were lysed by the addition of 30 μL of passive lysis buffer (Promega) followed by one freeze-thaw cycle ...
-
bioRxiv - Genomics 2023Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... transfected cells were washed once with PBS and lysed directly in passive cell lysis buffer (Promega). Cells were incubated on a rotating platform at room temperature for 10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Nuclei were resuspended in 35 µL of reaction buffer (0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
-
bioRxiv - Pathology 2024Quote: ... Cell Titer-Glo® Buffer and Substrate were mixed per the manufacturer’s instructions (Promega, Southampton, UK), and then added 100 µl to each well containing the treated cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... After the translation reaction was quenched by adding 30 μl of 1× Passive Lysis Buffer (Promega), 10 μl of the mixture was placed on a 96-well white assay plate (Coster) ...
-
bioRxiv - Neuroscience 2023Quote: ... At DIV12 cells are lysed in Passive lysis buffer (diluted 1:5 in MilliQ-water, Promega) for 15min and dual luciferase assay performed using home-made reagents according to Baker and Boyce (63 ...
-
bioRxiv - Biophysics 2023Quote: ... Cells were lysed 44-48 hours post-transfection by adding 250 μL Passive Lysis Buffer (Promega) to each well ...
-
bioRxiv - Genomics 2023Quote: ... 500 μl of CTAB lysis buffer and 10 μl of Proteinase K (20 mg/ml; Promega) were added to the sample and incubated at 65ºC for 90 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
-
bioRxiv - Synthetic Biology 2023Quote: ... then adding 30 μl of pre-mixed nGlo buffer with the recommended substrate concentration (Promega, #N1110). Luminescence was read in black opaque 96 well plates (Thermo Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... The luminescence was measured in a 384 well plate using NanoGlo® live cell buffer (Promega) supplemented with 1:50 of either a membrane impermeable substrate (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... after which they were lysed by the addition of 40 μL of passive lysis buffer (Promega) and one freeze-thaw cycle ...
-
bioRxiv - Genomics 2022Quote: ... the NE1 and Wash buffers were supplemented with 1 U/μL of RNasin Ribonuclease Inhibitor (Promega).
-
bioRxiv - Microbiology 2022Quote: ... the transfected cells were harvested and lysed using 200 µl of passive lysis buffer (#E1941, Promega). Luciferase (Fluc ...
-
bioRxiv - Biochemistry 2023Quote: ... the cells were washed with DBPS and lysed in 60 μL Passive lysis buffer (Promega, E1910). For measurement ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Two 8 mm-diameter discs per infiltrated leaf were homogenized in180 μL passive lysis buffer (Promega) containing protease inhibitor (P9599 ...
-
bioRxiv - Cell Biology 2022Quote: ... and 125 μL digestion buffer (50 mM triethylammonium bicarbonate; TEAB) supplemented with sequencing grade Trypsin (Promega) at a concentration of 1 μg trypsin ...
-
bioRxiv - Immunology 2023Quote: ... and the cells were lysed by the addition of 30 µl of passive lysis buffer (Promega) and three freeze-thaw cycles ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were lysed using RNA Lysis Buffer of SV Total RNA Isolation System (Promega, Madison, WI) for RNA quantification.
-
bioRxiv - Immunology 2024Quote: ... The cells were then left for 48 h before addition of lysis buffer + luciferase substrate (Promega) and read on a Glomax plate reader (Promega) ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were washed in serum–free media and lysed in Passive Lysis Buffer (Cat. #E1960; Promega). The luciferase activity obtained in each condition was recorded using the Dual–Luciferase Reporter System (Cat.#E1960 ...
-
bioRxiv - Genetics 2024Quote: ... PCR reactions were carried out in 20 µL-volumes containing 1x GoTaq® reaction buffer (Promega), 0.1 mM dNTPs ...
-
bioRxiv - Cell Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
-
bioRxiv - Neuroscience 2020Quote: ... CellTiter 96® Aqueous One Solution cell proliferation assay kit and ApoTox-Glo™ Triplex Assay kit was obtained from Promega, Australia ...
-
bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
-
bioRxiv - Cancer Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... Cell viability was measured 48h later (or otherwise indicated) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
CREB5 reprograms nuclear interactions to promote resistance to androgen receptor targeting therapiesbioRxiv - Cancer Biology 2021Quote: ... Rehydration of the beads was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... 1st to 5th instar nymphs, adult males and females) and different organs (antennae, brain, midgut, reproductive organs) using RNAgent® Denaturing Solution (Promega). The organs of H ...
-
bioRxiv - Cell Biology 2020Quote: ... and results were normalized to living cell numbers as evaluated with the CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... dried in Speed Vac and rehydrated in 20 μl of 25 mM NH4HCO3 solution containing 12.5 ng/μl trypsin (sequencing grade, Promega, Wisconsin, USA). This mixture was incubated on ice for 10 min and digested at 37 °C overnight ...
-
bioRxiv - Immunology 2020Quote: Viable cell numbers were quantified either by Trypan blue exclusion or by the production of formazan product (OD490) 2 hours after addition of CellTiter 96® Aqueous One Solution assay reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2021Quote: ... Development and stop solutions were added followed by detection of absorbance at 450 on a Luminometer: GloMax-Multi Detection System (Promega, USA).
-
bioRxiv - Cell Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Microbiology 2020Quote: The increase in caspase like enzymatic activity was evaluated by using a staining solution containing (FITC)-VAD-FMK (CaspACE™, Promega) at a final concentration of 10 µM/ml (74) ...
-
bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... hiPSC-aCM or HEK293 cells were incubated with a labeling solution containing both cell-impermeant HTL-Alexa488 (Promega G1001, 1 µM) and Cellmask Deep Red Plasma Membrane stain (Invitrogen C10046 ...
-
bioRxiv - Cell Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...